Elimination of D-lactate synthesis increases poly(3-hydroxybutyrate) and ethanol synthesis from glycerol and affects cofactor distribution in recombinant Escherichia coli

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The effect of eliminating D-lactate synthesis in poly(3-hydroxybutyrate) (PHB)-accumulating recombinant Escherichia coli (K24K) was analyzed using glycerol as a substrate. K24KL, an ldhA derivative, produced more biomass and had altered carbon partitioning among the metabolic products, probably due...

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Autores principales:	Nikel, P.I., Giordano, A.M., De Almeida, A., Godoy, M.S., Pettinari, M.J.
Formato:	Artículo publishedVersion
Publicado:	2010
Materias:	Carbon partitioning Carbon precursors Cofactors D-lactate Fed-batch cultures Metabolic products Poly(3-hydroxybutyrate) Recombinant Escherichia coli Reducing power Batch cell culture Escherichia coli Ethanol Substrates Glycerol alcohol carbon glycerol hydroxybutyric acid lactate dehydrogenase lactic acid nicotinamide adenine dinucleotide nicotinamide adenine dinucleotide phosphate poly(3 hydroxybutyric acid) poly-beta-hydroxybutyrate polyester biomass ester ethanol fecal coliform partitioning recombination article bioreactor chemistry genetics growth, development and aging metabolism time Biomass Bioreactors Carbon Hydroxybutyrates Lactate Dehydrogenases Lactic Acid Metabolic Networks and Pathways NAD NADP Polyesters Time Factors
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00992240_v76_n22_p7400_Nikel https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00992240_v76_n22_p7400_Nikel_oai
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

id	I28-R145-paper_00992240_v76_n22_p7400_Nikel_oai
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spelling	I28-R145-paper_00992240_v76_n22_p7400_Nikel_oai2024-08-16 Nikel, P.I. Giordano, A.M. De Almeida, A. Godoy, M.S. Pettinari, M.J. 2010 The effect of eliminating D-lactate synthesis in poly(3-hydroxybutyrate) (PHB)-accumulating recombinant Escherichia coli (K24K) was analyzed using glycerol as a substrate. K24KL, an ldhA derivative, produced more biomass and had altered carbon partitioning among the metabolic products, probably due to the increased availability of carbon precursors and reducing power. This resulted in a significant increase of PHB and ethanol synthesis and a decrease in acetate production. Cofactor measurements revealed that cultures of K24K and K24KL had a high intracellular NADPH content and that the NADPH/NADP+ ratio was higher than the NADH/NAD+ ratio. The ldhA mutation affected cofactor distribution, resulting in a more reduced intracellular state, mainly due to a further increase in NADPH/NADP+. In 60-h fed-batch cultures, K24KL reached 41.9 g · liter-1 biomass and accumulated PHB up to 63% ± 1% (wt/wt), with a PHB yield on glycerol of 0.41 ± 0.03 g · g-1, the highest reported using this substrate. © 2010, American Society for Microbiology. Fil:De Almeida, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_00992240_v76_n22_p7400_Nikel info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Appl. Environ. Microbiol. 2010;76(22):7400-7406 Carbon partitioning Carbon precursors Cofactors D-lactate Fed-batch cultures Metabolic products Poly(3-hydroxybutyrate) Recombinant Escherichia coli Reducing power Batch cell culture Escherichia coli Ethanol Substrates Glycerol alcohol carbon glycerol hydroxybutyric acid lactate dehydrogenase lactic acid nicotinamide adenine dinucleotide nicotinamide adenine dinucleotide phosphate poly(3 hydroxybutyric acid) poly-beta-hydroxybutyrate polyester alcohol biomass ester ethanol fecal coliform partitioning recombination article biomass bioreactor chemistry Escherichia coli genetics growth, development and aging metabolism time Biomass Bioreactors Carbon Escherichia coli Ethanol Glycerol Hydroxybutyrates Lactate Dehydrogenases Lactic Acid Metabolic Networks and Pathways NAD NADP Polyesters Time Factors Escherichia coli Elimination of D-lactate synthesis increases poly(3-hydroxybutyrate) and ethanol synthesis from glycerol and affects cofactor distribution in recombinant Escherichia coli info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00992240_v76_n22_p7400_Nikel_oai
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic	Carbon partitioning Carbon precursors Cofactors D-lactate Fed-batch cultures Metabolic products Poly(3-hydroxybutyrate) Recombinant Escherichia coli Reducing power Batch cell culture Escherichia coli Ethanol Substrates Glycerol alcohol carbon glycerol hydroxybutyric acid lactate dehydrogenase lactic acid nicotinamide adenine dinucleotide nicotinamide adenine dinucleotide phosphate poly(3 hydroxybutyric acid) poly-beta-hydroxybutyrate polyester alcohol biomass ester ethanol fecal coliform partitioning recombination article biomass bioreactor chemistry Escherichia coli genetics growth, development and aging metabolism time Biomass Bioreactors Carbon Escherichia coli Ethanol Glycerol Hydroxybutyrates Lactate Dehydrogenases Lactic Acid Metabolic Networks and Pathways NAD NADP Polyesters Time Factors Escherichia coli
spellingShingle	Carbon partitioning Carbon precursors Cofactors D-lactate Fed-batch cultures Metabolic products Poly(3-hydroxybutyrate) Recombinant Escherichia coli Reducing power Batch cell culture Escherichia coli Ethanol Substrates Glycerol alcohol carbon glycerol hydroxybutyric acid lactate dehydrogenase lactic acid nicotinamide adenine dinucleotide nicotinamide adenine dinucleotide phosphate poly(3 hydroxybutyric acid) poly-beta-hydroxybutyrate polyester alcohol biomass ester ethanol fecal coliform partitioning recombination article biomass bioreactor chemistry Escherichia coli genetics growth, development and aging metabolism time Biomass Bioreactors Carbon Escherichia coli Ethanol Glycerol Hydroxybutyrates Lactate Dehydrogenases Lactic Acid Metabolic Networks and Pathways NAD NADP Polyesters Time Factors Escherichia coli Nikel, P.I. Giordano, A.M. De Almeida, A. Godoy, M.S. Pettinari, M.J. Elimination of D-lactate synthesis increases poly(3-hydroxybutyrate) and ethanol synthesis from glycerol and affects cofactor distribution in recombinant Escherichia coli
topic_facet	Carbon partitioning Carbon precursors Cofactors D-lactate Fed-batch cultures Metabolic products Poly(3-hydroxybutyrate) Recombinant Escherichia coli Reducing power Batch cell culture Escherichia coli Ethanol Substrates Glycerol alcohol carbon glycerol hydroxybutyric acid lactate dehydrogenase lactic acid nicotinamide adenine dinucleotide nicotinamide adenine dinucleotide phosphate poly(3 hydroxybutyric acid) poly-beta-hydroxybutyrate polyester alcohol biomass ester ethanol fecal coliform partitioning recombination article biomass bioreactor chemistry Escherichia coli genetics growth, development and aging metabolism time Biomass Bioreactors Carbon Escherichia coli Ethanol Glycerol Hydroxybutyrates Lactate Dehydrogenases Lactic Acid Metabolic Networks and Pathways NAD NADP Polyesters Time Factors Escherichia coli
description	The effect of eliminating D-lactate synthesis in poly(3-hydroxybutyrate) (PHB)-accumulating recombinant Escherichia coli (K24K) was analyzed using glycerol as a substrate. K24KL, an ldhA derivative, produced more biomass and had altered carbon partitioning among the metabolic products, probably due to the increased availability of carbon precursors and reducing power. This resulted in a significant increase of PHB and ethanol synthesis and a decrease in acetate production. Cofactor measurements revealed that cultures of K24K and K24KL had a high intracellular NADPH content and that the NADPH/NADP+ ratio was higher than the NADH/NAD+ ratio. The ldhA mutation affected cofactor distribution, resulting in a more reduced intracellular state, mainly due to a further increase in NADPH/NADP+. In 60-h fed-batch cultures, K24KL reached 41.9 g · liter-1 biomass and accumulated PHB up to 63% ± 1% (wt/wt), with a PHB yield on glycerol of 0.41 ± 0.03 g · g-1, the highest reported using this substrate. © 2010, American Society for Microbiology.
format	Artículo Artículo publishedVersion
author	Nikel, P.I. Giordano, A.M. De Almeida, A. Godoy, M.S. Pettinari, M.J.
author_facet	Nikel, P.I. Giordano, A.M. De Almeida, A. Godoy, M.S. Pettinari, M.J.
author_sort	Nikel, P.I.
title	Elimination of D-lactate synthesis increases poly(3-hydroxybutyrate) and ethanol synthesis from glycerol and affects cofactor distribution in recombinant Escherichia coli
title_short	Elimination of D-lactate synthesis increases poly(3-hydroxybutyrate) and ethanol synthesis from glycerol and affects cofactor distribution in recombinant Escherichia coli
title_full	Elimination of D-lactate synthesis increases poly(3-hydroxybutyrate) and ethanol synthesis from glycerol and affects cofactor distribution in recombinant Escherichia coli
title_fullStr	Elimination of D-lactate synthesis increases poly(3-hydroxybutyrate) and ethanol synthesis from glycerol and affects cofactor distribution in recombinant Escherichia coli
title_full_unstemmed	Elimination of D-lactate synthesis increases poly(3-hydroxybutyrate) and ethanol synthesis from glycerol and affects cofactor distribution in recombinant Escherichia coli
title_sort	elimination of d-lactate synthesis increases poly(3-hydroxybutyrate) and ethanol synthesis from glycerol and affects cofactor distribution in recombinant escherichia coli
publishDate	2010
url	http://hdl.handle.net/20.500.12110/paper_00992240_v76_n22_p7400_Nikel https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00992240_v76_n22_p7400_Nikel_oai
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Elimination of D-lactate synthesis increases poly(3-hydroxybutyrate) and ethanol synthesis from glycerol and affects cofactor distribution in recombinant Escherichia coli

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