New recombinant Escherichia coli strain tailored for the production of poly(3-hydroxybutyrate) from agroindustrial by-products

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A recombinant E. coli strain (K24K) was constructed and evaluated For poly(3-hydroxybutyrate) (PHB) production from whey and corn steep liquor as main carbon and nitrogen sources. This strain bears the pha biosynthetic genes from Azotobacter sp. strain FA8 expressed from a T5 promoter under the cont...

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Autores principales:	Nikel, P.I., De Almeida, A., Melillo, E.C., Galvagno, M.A., Pettinari, M.J.
Formato:	Artículo publishedVersion
Publicado:	2006
Materias:	Bioreactors Carbon Cells Genes Glass transition Molecular weight Nitrogen Polymers Volumetric analysis Azotobacter Cupriavidus necator Lactose operator Liquor Poly(3-hydroxybutyrate) (PHB) Escherichia coli carbon lactose lactose repressor nitrogen poly(3 hydroxybutyric acid) polymer unclassified drug bacterium ester gene expression genetic analysis plant extract agriculture alcoholic beverage article bacterial gene bacterial strain bioreactor corn Escherichia coli k24k evaluation fed batch culture glass transition temperature liquid molecular weight nonhuman pha gene productivity repressor gene volumetry Wautersia eutropha whey Agriculture Animals DNA Primers Hydroxybutyrates Industrial Waste Kinetics Milk Plasmids Polyesters Recombination, Genetic Zea mays
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00992240_v72_n6_p3949_Nikel http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00992240_v72_n6_p3949_Nikel_oai
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

id	I28-R145-paper_00992240_v72_n6_p3949_Nikel_oai
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spelling	I28-R145-paper_00992240_v72_n6_p3949_Nikel_oai2020-10-19 Nikel, P.I. De Almeida, A. Melillo, E.C. Galvagno, M.A. Pettinari, M.J. 2006 A recombinant E. coli strain (K24K) was constructed and evaluated For poly(3-hydroxybutyrate) (PHB) production from whey and corn steep liquor as main carbon and nitrogen sources. This strain bears the pha biosynthetic genes from Azotobacter sp. strain FA8 expressed from a T5 promoter under the control of the lactose operator. K24K does not produce the lactose repressor, ensuring constitutive expression of genes involved in lactose transport and utilization. PHB was efficiently produced by the recombinant strain grown aerobically in fed-batch cultures in a laboratory scale bioreactor on a semisynthetic medium supplemented with the agroindustrial by-products. After 24 h, cells accumulated PHB to 72.9% of their cell dry weight, reaching a volumetric productivity of 2.13 g PHB per liter per hour. Physical analysis of PHB recovered from the recombinants showed that its molecular weight was similar to that of PHB produced by Azotobacter sp. strain FA8 and higher than that of the polymer from Cupriavidus necator and that its glass transition temperature was approximately 20°C higher than those of PHBs from the natural producer strains. Copyright © 2006, American Society for Microbiology. All Rights Reserved. Fil:De Almeida, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Galvagno, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_00992240_v72_n6_p3949_Nikel info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Appl. Environ. Microbiol. 2006;72(6):3949-3954 Bioreactors Carbon Cells Genes Glass transition Molecular weight Nitrogen Polymers Volumetric analysis Azotobacter Cupriavidus necator Lactose operator Liquor Poly(3-hydroxybutyrate) (PHB) Escherichia coli carbon lactose lactose repressor nitrogen poly(3 hydroxybutyric acid) polymer unclassified drug bacterium ester gene expression genetic analysis plant extract agriculture alcoholic beverage article Azotobacter bacterial gene bacterial strain bioreactor corn Escherichia coli Escherichia coli k24k evaluation fed batch culture gene expression glass transition temperature liquid molecular weight nonhuman pha gene productivity repressor gene volumetry Wautersia eutropha whey Agriculture Animals Azotobacter DNA Primers Escherichia coli Hydroxybutyrates Industrial Waste Kinetics Milk Plasmids Polyesters Recombination, Genetic Zea mays Azotobacter Cupriavidus necator Escherichia coli Zea mays New recombinant Escherichia coli strain tailored for the production of poly(3-hydroxybutyrate) from agroindustrial by-products info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00992240_v72_n6_p3949_Nikel_oai
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic	Bioreactors Carbon Cells Genes Glass transition Molecular weight Nitrogen Polymers Volumetric analysis Azotobacter Cupriavidus necator Lactose operator Liquor Poly(3-hydroxybutyrate) (PHB) Escherichia coli carbon lactose lactose repressor nitrogen poly(3 hydroxybutyric acid) polymer unclassified drug bacterium ester gene expression genetic analysis plant extract agriculture alcoholic beverage article Azotobacter bacterial gene bacterial strain bioreactor corn Escherichia coli Escherichia coli k24k evaluation fed batch culture gene expression glass transition temperature liquid molecular weight nonhuman pha gene productivity repressor gene volumetry Wautersia eutropha whey Agriculture Animals Azotobacter DNA Primers Escherichia coli Hydroxybutyrates Industrial Waste Kinetics Milk Plasmids Polyesters Recombination, Genetic Zea mays Azotobacter Cupriavidus necator Escherichia coli Zea mays
spellingShingle	Bioreactors Carbon Cells Genes Glass transition Molecular weight Nitrogen Polymers Volumetric analysis Azotobacter Cupriavidus necator Lactose operator Liquor Poly(3-hydroxybutyrate) (PHB) Escherichia coli carbon lactose lactose repressor nitrogen poly(3 hydroxybutyric acid) polymer unclassified drug bacterium ester gene expression genetic analysis plant extract agriculture alcoholic beverage article Azotobacter bacterial gene bacterial strain bioreactor corn Escherichia coli Escherichia coli k24k evaluation fed batch culture gene expression glass transition temperature liquid molecular weight nonhuman pha gene productivity repressor gene volumetry Wautersia eutropha whey Agriculture Animals Azotobacter DNA Primers Escherichia coli Hydroxybutyrates Industrial Waste Kinetics Milk Plasmids Polyesters Recombination, Genetic Zea mays Azotobacter Cupriavidus necator Escherichia coli Zea mays Nikel, P.I. De Almeida, A. Melillo, E.C. Galvagno, M.A. Pettinari, M.J. New recombinant Escherichia coli strain tailored for the production of poly(3-hydroxybutyrate) from agroindustrial by-products
topic_facet	Bioreactors Carbon Cells Genes Glass transition Molecular weight Nitrogen Polymers Volumetric analysis Azotobacter Cupriavidus necator Lactose operator Liquor Poly(3-hydroxybutyrate) (PHB) Escherichia coli carbon lactose lactose repressor nitrogen poly(3 hydroxybutyric acid) polymer unclassified drug bacterium ester gene expression genetic analysis plant extract agriculture alcoholic beverage article Azotobacter bacterial gene bacterial strain bioreactor corn Escherichia coli Escherichia coli k24k evaluation fed batch culture gene expression glass transition temperature liquid molecular weight nonhuman pha gene productivity repressor gene volumetry Wautersia eutropha whey Agriculture Animals Azotobacter DNA Primers Escherichia coli Hydroxybutyrates Industrial Waste Kinetics Milk Plasmids Polyesters Recombination, Genetic Zea mays Azotobacter Cupriavidus necator Escherichia coli Zea mays
description	A recombinant E. coli strain (K24K) was constructed and evaluated For poly(3-hydroxybutyrate) (PHB) production from whey and corn steep liquor as main carbon and nitrogen sources. This strain bears the pha biosynthetic genes from Azotobacter sp. strain FA8 expressed from a T5 promoter under the control of the lactose operator. K24K does not produce the lactose repressor, ensuring constitutive expression of genes involved in lactose transport and utilization. PHB was efficiently produced by the recombinant strain grown aerobically in fed-batch cultures in a laboratory scale bioreactor on a semisynthetic medium supplemented with the agroindustrial by-products. After 24 h, cells accumulated PHB to 72.9% of their cell dry weight, reaching a volumetric productivity of 2.13 g PHB per liter per hour. Physical analysis of PHB recovered from the recombinants showed that its molecular weight was similar to that of PHB produced by Azotobacter sp. strain FA8 and higher than that of the polymer from Cupriavidus necator and that its glass transition temperature was approximately 20°C higher than those of PHBs from the natural producer strains. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
format	Artículo Artículo publishedVersion
author	Nikel, P.I. De Almeida, A. Melillo, E.C. Galvagno, M.A. Pettinari, M.J.
author_facet	Nikel, P.I. De Almeida, A. Melillo, E.C. Galvagno, M.A. Pettinari, M.J.
author_sort	Nikel, P.I.
title	New recombinant Escherichia coli strain tailored for the production of poly(3-hydroxybutyrate) from agroindustrial by-products
title_short	New recombinant Escherichia coli strain tailored for the production of poly(3-hydroxybutyrate) from agroindustrial by-products
title_full	New recombinant Escherichia coli strain tailored for the production of poly(3-hydroxybutyrate) from agroindustrial by-products
title_fullStr	New recombinant Escherichia coli strain tailored for the production of poly(3-hydroxybutyrate) from agroindustrial by-products
title_full_unstemmed	New recombinant Escherichia coli strain tailored for the production of poly(3-hydroxybutyrate) from agroindustrial by-products
title_sort	new recombinant escherichia coli strain tailored for the production of poly(3-hydroxybutyrate) from agroindustrial by-products
publishDate	2006
url	http://hdl.handle.net/20.500.12110/paper_00992240_v72_n6_p3949_Nikel http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00992240_v72_n6_p3949_Nikel_oai
work_keys_str_mv	AT nikelpi newrecombinantescherichiacolistraintailoredfortheproductionofpoly3hydroxybutyratefromagroindustrialbyproducts AT dealmeidaa newrecombinantescherichiacolistraintailoredfortheproductionofpoly3hydroxybutyratefromagroindustrialbyproducts AT melilloec newrecombinantescherichiacolistraintailoredfortheproductionofpoly3hydroxybutyratefromagroindustrialbyproducts AT galvagnoma newrecombinantescherichiacolistraintailoredfortheproductionofpoly3hydroxybutyratefromagroindustrialbyproducts AT pettinarimj newrecombinantescherichiacolistraintailoredfortheproductionofpoly3hydroxybutyratefromagroindustrialbyproducts
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New recombinant Escherichia coli strain tailored for the production of poly(3-hydroxybutyrate) from agroindustrial by-products

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