Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8
Genes responsible for the synthesis of poly(3-hydroxybutyrate) (PHB) in Azotobacter sp. FA8 were cloned and analyzed. A PHB polymerase gene (phbC) was found downstream from genes coding for β-ketothiolase (phbA) and acetoacetyl-coenzyme A reductase (phbB). A PHB synthase mutant was obtained by gene...
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2001
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00992240_v67_n3-12_p5331_Pettinari http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00992240_v67_n3-12_p5331_Pettinari_oai |
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I28-R145-paper_00992240_v67_n3-12_p5331_Pettinari_oai2020-10-19 Pettinari, M.J. Vázquez, G.J. Silberschmidt, D. Rehm, B. Steibüchel, A. Méndez, B.S. 2001 Genes responsible for the synthesis of poly(3-hydroxybutyrate) (PHB) in Azotobacter sp. FA8 were cloned and analyzed. A PHB polymerase gene (phbC) was found downstream from genes coding for β-ketothiolase (phbA) and acetoacetyl-coenzyme A reductase (phbB). A PHB synthase mutant was obtained by gene inactivation and used for genetic studies. The phbC gene from this strain was introduced into Raktonia eutropha PHB-4 (phbC-negative mutant), and the recombinant accumulated PHB when either glucose or oclanoate was used as a source of carbon, indicating that this PHB synthase cannot incorporate medium-chuin-length hydroxyalkanoates into PHB. Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vázquez, G.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Silberschmidt, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_00992240_v67_n3-12_p5331_Pettinari info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Appl. Environ. Microbiol. 2001;67(3-12):5331-5334 acetoacetyl coenzyme a reductase acetoacetyl-CoA reductase acetyl coenzyme A acyltransferase acyltransferase alcohol dehydrogenase hydroxybutyric acid poly beta hydroxybutyrate polymerase poly(3 hydroxybutyric acid) poly-beta-hydroxybutyrate poly-beta-hydroxybutyrate polymerase polyester article Azotobacter bacterial gene DNA sequence gene deletion genetics growth, development and aging metabolism molecular cloning molecular genetics Acetyl-CoA C-Acyltransferase Acyltransferases Alcohol Oxidoreductases Azotobacter Cloning, Molecular Gene Deletion Genes, Bacterial Hydroxybutyrates Molecular Sequence Data Polyesters Sequence Analysis, DNA Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00992240_v67_n3-12_p5331_Pettinari_oai |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| topic |
acetoacetyl coenzyme a reductase acetoacetyl-CoA reductase acetyl coenzyme A acyltransferase acyltransferase alcohol dehydrogenase hydroxybutyric acid poly beta hydroxybutyrate polymerase poly(3 hydroxybutyric acid) poly-beta-hydroxybutyrate poly-beta-hydroxybutyrate polymerase polyester article Azotobacter bacterial gene DNA sequence gene deletion genetics growth, development and aging metabolism molecular cloning molecular genetics Acetyl-CoA C-Acyltransferase Acyltransferases Alcohol Oxidoreductases Azotobacter Cloning, Molecular Gene Deletion Genes, Bacterial Hydroxybutyrates Molecular Sequence Data Polyesters Sequence Analysis, DNA |
| spellingShingle |
acetoacetyl coenzyme a reductase acetoacetyl-CoA reductase acetyl coenzyme A acyltransferase acyltransferase alcohol dehydrogenase hydroxybutyric acid poly beta hydroxybutyrate polymerase poly(3 hydroxybutyric acid) poly-beta-hydroxybutyrate poly-beta-hydroxybutyrate polymerase polyester article Azotobacter bacterial gene DNA sequence gene deletion genetics growth, development and aging metabolism molecular cloning molecular genetics Acetyl-CoA C-Acyltransferase Acyltransferases Alcohol Oxidoreductases Azotobacter Cloning, Molecular Gene Deletion Genes, Bacterial Hydroxybutyrates Molecular Sequence Data Polyesters Sequence Analysis, DNA Pettinari, M.J. Vázquez, G.J. Silberschmidt, D. Rehm, B. Steibüchel, A. Méndez, B.S. Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8 |
| topic_facet |
acetoacetyl coenzyme a reductase acetoacetyl-CoA reductase acetyl coenzyme A acyltransferase acyltransferase alcohol dehydrogenase hydroxybutyric acid poly beta hydroxybutyrate polymerase poly(3 hydroxybutyric acid) poly-beta-hydroxybutyrate poly-beta-hydroxybutyrate polymerase polyester article Azotobacter bacterial gene DNA sequence gene deletion genetics growth, development and aging metabolism molecular cloning molecular genetics Acetyl-CoA C-Acyltransferase Acyltransferases Alcohol Oxidoreductases Azotobacter Cloning, Molecular Gene Deletion Genes, Bacterial Hydroxybutyrates Molecular Sequence Data Polyesters Sequence Analysis, DNA |
| description |
Genes responsible for the synthesis of poly(3-hydroxybutyrate) (PHB) in Azotobacter sp. FA8 were cloned and analyzed. A PHB polymerase gene (phbC) was found downstream from genes coding for β-ketothiolase (phbA) and acetoacetyl-coenzyme A reductase (phbB). A PHB synthase mutant was obtained by gene inactivation and used for genetic studies. The phbC gene from this strain was introduced into Raktonia eutropha PHB-4 (phbC-negative mutant), and the recombinant accumulated PHB when either glucose or oclanoate was used as a source of carbon, indicating that this PHB synthase cannot incorporate medium-chuin-length hydroxyalkanoates into PHB. |
| format |
Artículo Artículo publishedVersion |
| author |
Pettinari, M.J. Vázquez, G.J. Silberschmidt, D. Rehm, B. Steibüchel, A. Méndez, B.S. |
| author_facet |
Pettinari, M.J. Vázquez, G.J. Silberschmidt, D. Rehm, B. Steibüchel, A. Méndez, B.S. |
| author_sort |
Pettinari, M.J. |
| title |
Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8 |
| title_short |
Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8 |
| title_full |
Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8 |
| title_fullStr |
Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8 |
| title_full_unstemmed |
Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8 |
| title_sort |
poly(3-hydroxybutyrate) synthesis genes in azotobacter sp. strain fa8 |
| publishDate |
2001 |
| url |
http://hdl.handle.net/20.500.12110/paper_00992240_v67_n3-12_p5331_Pettinari http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00992240_v67_n3-12_p5331_Pettinari_oai |
| work_keys_str_mv |
AT pettinarimj poly3hydroxybutyratesynthesisgenesinazotobacterspstrainfa8 AT vazquezgj poly3hydroxybutyratesynthesisgenesinazotobacterspstrainfa8 AT silberschmidtd poly3hydroxybutyratesynthesisgenesinazotobacterspstrainfa8 AT rehmb poly3hydroxybutyratesynthesisgenesinazotobacterspstrainfa8 AT steibuchela poly3hydroxybutyratesynthesisgenesinazotobacterspstrainfa8 AT mendezbs poly3hydroxybutyratesynthesisgenesinazotobacterspstrainfa8 |
| _version_ |
1766026654332747776 |