Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G

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The combined effect of water activity (a(w)) and pH on growth and toxin production by Clostridium botulinum type G strain 89 was investigated. The minimum a(w) at which growth and toxin formation occurred was 0.965, for media in which the pH was adjusted with either sodium chloride or sucrose. The m...

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Autores principales:	Briozzo, J., Amato de Lagarde, E., Chirife, J., Parada, J.L.
Formato:	Artículo publishedVersion
Publicado:	1986
Materias:	botulinum toxin water activity bacterial growth clostridium botulinum type g nonhuman ph priority journal Botulinum Toxins Clostridium botulinum Culture Media Heat Hydrogen-Ion Concentration Support, Non-U.S. Gov't Trypsin Water
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00992240_v51_n4_p844_Briozzo https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00992240_v51_n4_p844_Briozzo_oai
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

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spelling	I28-R145-paper_00992240_v51_n4_p844_Briozzo_oai2024-08-16 Briozzo, J. Amato de Lagarde, E. Chirife, J. Parada, J.L. 1986 The combined effect of water activity (a(w)) and pH on growth and toxin production by Clostridium botulinum type G strain 89 was investigated. The minimum a(w) at which growth and toxin formation occurred was 0.965, for media in which the pH was adjusted with either sodium chloride or sucrose. The minimum pH (at the optimum a(w)) for growth and toxin production of C. botulinum type G was found to be 5.6. Optimum conditions for toxin activation were a trypsin concentration of 0.1%, a pH of the medium of 6.5, and an incubation for 45 min at 37° C. These data did not show evidence of heat-labile spores, since a heat shock of 75°C for 10 min did not significantly decrease the spore count of strain 89G in media at pH 7.0 or 5.6. It was frequently observed that cells grown at reduced a(w) or pH experienced severe morphological changes. Fil:Briozzo, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_00992240_v51_n4_p844_Briozzo info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar APPL. ENVIRON. MICROBIOL. 1986;51(4):844-848 botulinum toxin water activity bacterial growth clostridium botulinum type g nonhuman ph priority journal Botulinum Toxins Clostridium botulinum Culture Media Heat Hydrogen-Ion Concentration Support, Non-U.S. Gov't Trypsin Water Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00992240_v51_n4_p844_Briozzo_oai
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic	botulinum toxin water activity bacterial growth clostridium botulinum type g nonhuman ph priority journal Botulinum Toxins Clostridium botulinum Culture Media Heat Hydrogen-Ion Concentration Support, Non-U.S. Gov't Trypsin Water
spellingShingle	botulinum toxin water activity bacterial growth clostridium botulinum type g nonhuman ph priority journal Botulinum Toxins Clostridium botulinum Culture Media Heat Hydrogen-Ion Concentration Support, Non-U.S. Gov't Trypsin Water Briozzo, J. Amato de Lagarde, E. Chirife, J. Parada, J.L. Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G
topic_facet	botulinum toxin water activity bacterial growth clostridium botulinum type g nonhuman ph priority journal Botulinum Toxins Clostridium botulinum Culture Media Heat Hydrogen-Ion Concentration Support, Non-U.S. Gov't Trypsin Water
description	The combined effect of water activity (a(w)) and pH on growth and toxin production by Clostridium botulinum type G strain 89 was investigated. The minimum a(w) at which growth and toxin formation occurred was 0.965, for media in which the pH was adjusted with either sodium chloride or sucrose. The minimum pH (at the optimum a(w)) for growth and toxin production of C. botulinum type G was found to be 5.6. Optimum conditions for toxin activation were a trypsin concentration of 0.1%, a pH of the medium of 6.5, and an incubation for 45 min at 37° C. These data did not show evidence of heat-labile spores, since a heat shock of 75°C for 10 min did not significantly decrease the spore count of strain 89G in media at pH 7.0 or 5.6. It was frequently observed that cells grown at reduced a(w) or pH experienced severe morphological changes.
format	Artículo Artículo publishedVersion
author	Briozzo, J. Amato de Lagarde, E. Chirife, J. Parada, J.L.
author_facet	Briozzo, J. Amato de Lagarde, E. Chirife, J. Parada, J.L.
author_sort	Briozzo, J.
title	Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G
title_short	Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G
title_full	Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G
title_fullStr	Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G
title_full_unstemmed	Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G
title_sort	effect of water activity and ph on growth and toxin production by clostridium botulinum type g
publishDate	1986
url	http://hdl.handle.net/20.500.12110/paper_00992240_v51_n4_p844_Briozzo https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00992240_v51_n4_p844_Briozzo_oai
work_keys_str_mv	AT briozzoj effectofwateractivityandphongrowthandtoxinproductionbyclostridiumbotulinumtypeg AT amatodelagardee effectofwateractivityandphongrowthandtoxinproductionbyclostridiumbotulinumtypeg AT chirifej effectofwateractivityandphongrowthandtoxinproductionbyclostridiumbotulinumtypeg AT paradajl effectofwateractivityandphongrowthandtoxinproductionbyclostridiumbotulinumtypeg
_version_	1809356751616606208

Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G

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