Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina
We studied hantavirus seroprevalence and virus variability in rodent populations in Diego Gaynor, northwest of Buenos Aires province, Argentina. Rodent samplings were conducted in railroads and cropfield borders in March and July 1999, September and December 2000, and March 2001. Antibody detection...
Guardado en:
| Autores principales: | , , , , , , , , , , , |
|---|---|
| Formato: | Artículo publishedVersion |
| Publicado: |
2003
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00740276_v98_n6_p727_Suarez https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00740276_v98_n6_p727_Suarez_oai |
| Aporte de: |
| id |
I28-R145-paper_00740276_v98_n6_p727_Suarez_oai |
|---|---|
| record_format |
dspace |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| topic |
Argentina Hantavirus pulmonary syndrome Seroprevalence Sigmodontine rodents nucleoprotein recombinant protein virus antigen virus protein antibody detection Argentina article enzyme linked immunosorbent assay gene sequence genotype Hantavirus Hantavirus pulmonary syndrome histopathology molecular biology nonhuman railway rodent seroprevalence species comparison virus genome virus identification Akodon Akodon azarae Andes virus Calomys Calomys laucha Calomys musculinus Convict Creek 107 virus Hantaan virus Lee Hantavirus Oligoryzomys Oligoryzomys flavescens Pergamino virus Rodentia |
| spellingShingle |
Argentina Hantavirus pulmonary syndrome Seroprevalence Sigmodontine rodents nucleoprotein recombinant protein virus antigen virus protein antibody detection Argentina article enzyme linked immunosorbent assay gene sequence genotype Hantavirus Hantavirus pulmonary syndrome histopathology molecular biology nonhuman railway rodent seroprevalence species comparison virus genome virus identification Akodon Akodon azarae Andes virus Calomys Calomys laucha Calomys musculinus Convict Creek 107 virus Hantaan virus Lee Hantavirus Oligoryzomys Oligoryzomys flavescens Pergamino virus Rodentia Suárez, O.V. Cueto, G.R. Cavia, R. Villafañe, I.E.G. Bilenca, D.N. Edelstein, A. Martínez, P. Miguel, S. Bellomo, C. Hodara, K. Padula, P.J. Busch, M. Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina |
| topic_facet |
Argentina Hantavirus pulmonary syndrome Seroprevalence Sigmodontine rodents nucleoprotein recombinant protein virus antigen virus protein antibody detection Argentina article enzyme linked immunosorbent assay gene sequence genotype Hantavirus Hantavirus pulmonary syndrome histopathology molecular biology nonhuman railway rodent seroprevalence species comparison virus genome virus identification Akodon Akodon azarae Andes virus Calomys Calomys laucha Calomys musculinus Convict Creek 107 virus Hantaan virus Lee Hantavirus Oligoryzomys Oligoryzomys flavescens Pergamino virus Rodentia |
| description |
We studied hantavirus seroprevalence and virus variability in rodent populations in Diego Gaynor, northwest of Buenos Aires province, Argentina. Rodent samplings were conducted in railroads and cropfield borders in March and July 1999, September and December 2000, and March 2001. Antibody detection was performed by an enzyme link immunosorbent assay (ELISA), using the recombinant nucleoprotein of Andes (AND) virus as antigen. Tissue samples were taken from positive antibody individuals in order to confirm the presence of hantavirus genomic material and to identify virus genotypes. Akodon azarae was the most abundant species, followed by Oligoryzomys flavescens, while Calomys laucha and C. musculinus were rarely caught. We found a rate of seroprevalence of 9.3% for a total sample of 291 A. azarae and 13.5% for 37 O. flavescens. After molecular analyses of hantavirus, we confirmed the presence of hantavirus genomic material in 16 individuals with ELISA (+) results and two individuals with ELISA (-). Four amplimers for each species were sequenced and compared to the corresponding sequences of representative hantaviruses. We identified the AND Cent Lee from three O. flavescens, and the Pergamino virus from four A. azarae and from one O. flavescens. A. azarae males had higher seroprevalence than females, and heavier individuals showed higher seroprevalence than lighter ones. We did not find seroprevalence differences according to sex in O. flavescens, although this result may have been produced by the low sample size. The lowest seroprevalence was found in a period of high rodent density, when juveniles prevailed in the population. We found higher seroprevalences than those detected in previous studies for other localities of central Argentina where cases of hantavirus pulmonary syndrome (HPS) have been reported. The presence of AND Cent Lee virus in rodent populations of the study area, which is responsible of HPS cases in central Argentina, suggests that human populations are at risk of HPS disease, although there were not reported cases of this disease until today. |
| format |
Artículo Artículo publishedVersion |
| author |
Suárez, O.V. Cueto, G.R. Cavia, R. Villafañe, I.E.G. Bilenca, D.N. Edelstein, A. Martínez, P. Miguel, S. Bellomo, C. Hodara, K. Padula, P.J. Busch, M. |
| author_facet |
Suárez, O.V. Cueto, G.R. Cavia, R. Villafañe, I.E.G. Bilenca, D.N. Edelstein, A. Martínez, P. Miguel, S. Bellomo, C. Hodara, K. Padula, P.J. Busch, M. |
| author_sort |
Suárez, O.V. |
| title |
Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina |
| title_short |
Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina |
| title_full |
Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina |
| title_fullStr |
Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina |
| title_full_unstemmed |
Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina |
| title_sort |
prevalence of infection with hantavirus in rodent populations of central argentina |
| publishDate |
2003 |
| url |
http://hdl.handle.net/20.500.12110/paper_00740276_v98_n6_p727_Suarez https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00740276_v98_n6_p727_Suarez_oai |
| work_keys_str_mv |
AT suarezov prevalenceofinfectionwithhantavirusinrodentpopulationsofcentralargentina AT cuetogr prevalenceofinfectionwithhantavirusinrodentpopulationsofcentralargentina AT caviar prevalenceofinfectionwithhantavirusinrodentpopulationsofcentralargentina AT villafaneieg prevalenceofinfectionwithhantavirusinrodentpopulationsofcentralargentina AT bilencadn prevalenceofinfectionwithhantavirusinrodentpopulationsofcentralargentina AT edelsteina prevalenceofinfectionwithhantavirusinrodentpopulationsofcentralargentina AT martinezp prevalenceofinfectionwithhantavirusinrodentpopulationsofcentralargentina AT miguels prevalenceofinfectionwithhantavirusinrodentpopulationsofcentralargentina AT bellomoc prevalenceofinfectionwithhantavirusinrodentpopulationsofcentralargentina AT hodarak prevalenceofinfectionwithhantavirusinrodentpopulationsofcentralargentina AT padulapj prevalenceofinfectionwithhantavirusinrodentpopulationsofcentralargentina AT buschm prevalenceofinfectionwithhantavirusinrodentpopulationsofcentralargentina |
| _version_ |
1809357084130541568 |
| spelling |
I28-R145-paper_00740276_v98_n6_p727_Suarez_oai2024-08-16 Suárez, O.V. Cueto, G.R. Cavia, R. Villafañe, I.E.G. Bilenca, D.N. Edelstein, A. Martínez, P. Miguel, S. Bellomo, C. Hodara, K. Padula, P.J. Busch, M. 2003 We studied hantavirus seroprevalence and virus variability in rodent populations in Diego Gaynor, northwest of Buenos Aires province, Argentina. Rodent samplings were conducted in railroads and cropfield borders in March and July 1999, September and December 2000, and March 2001. Antibody detection was performed by an enzyme link immunosorbent assay (ELISA), using the recombinant nucleoprotein of Andes (AND) virus as antigen. Tissue samples were taken from positive antibody individuals in order to confirm the presence of hantavirus genomic material and to identify virus genotypes. Akodon azarae was the most abundant species, followed by Oligoryzomys flavescens, while Calomys laucha and C. musculinus were rarely caught. We found a rate of seroprevalence of 9.3% for a total sample of 291 A. azarae and 13.5% for 37 O. flavescens. After molecular analyses of hantavirus, we confirmed the presence of hantavirus genomic material in 16 individuals with ELISA (+) results and two individuals with ELISA (-). Four amplimers for each species were sequenced and compared to the corresponding sequences of representative hantaviruses. We identified the AND Cent Lee from three O. flavescens, and the Pergamino virus from four A. azarae and from one O. flavescens. A. azarae males had higher seroprevalence than females, and heavier individuals showed higher seroprevalence than lighter ones. We did not find seroprevalence differences according to sex in O. flavescens, although this result may have been produced by the low sample size. The lowest seroprevalence was found in a period of high rodent density, when juveniles prevailed in the population. We found higher seroprevalences than those detected in previous studies for other localities of central Argentina where cases of hantavirus pulmonary syndrome (HPS) have been reported. The presence of AND Cent Lee virus in rodent populations of the study area, which is responsible of HPS cases in central Argentina, suggests that human populations are at risk of HPS disease, although there were not reported cases of this disease until today. Fil:Suárez, O.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cueto, G.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cavia, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bilenca, D.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Edelstein, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hodara, K. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Padula, P.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Busch, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_00740276_v98_n6_p727_Suarez info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Mem. Inst. Oswaldo Cruz 2003;98(6):727-732 Argentina Hantavirus pulmonary syndrome Seroprevalence Sigmodontine rodents nucleoprotein recombinant protein virus antigen virus protein antibody detection Argentina article enzyme linked immunosorbent assay gene sequence genotype Hantavirus Hantavirus pulmonary syndrome histopathology molecular biology nonhuman railway rodent seroprevalence species comparison virus genome virus identification Akodon Akodon azarae Andes virus Calomys Calomys laucha Calomys musculinus Convict Creek 107 virus Hantaan virus Lee Hantavirus Oligoryzomys Oligoryzomys flavescens Pergamino virus Rodentia Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00740276_v98_n6_p727_Suarez_oai |