Relationships in Patagonian species of Berberis (Berberidaceae) based on the characterization of rDNA internal transcribed spacer sequences

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Sequence analysis of the internal transcribed spacer (ITS) of the 18S(ITS1)-5.8S-26S(ITS2) rDNA region was performed in order to analyse the phylogenetic relationships between 13 Patagonian species of the genus Berberis (Berberidaceae). The divergence values between the pairwise sequence in the stud...

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Detalles Bibliográficos
Autores principales: Bottini, M.C.J., De Bustos, A., Sanso, A.M., Jouve, N., Poggio, L.
Formato: Artículo publishedVersion
Publicado: 2007
Materias:
Argentina
Barberry
Calafate
Diploid and polyploid hybrid speciation
Michay
Molecular markers
DNA fingerprinting
genetic differentiation
hybridization
molecular analysis
phylogenetics
polymorphism
polyploidy
shrub
speciation (biology)
South America
Berberidaceae
Berberis
Berberis buxifolia
Berberis heterophylla
Berberis microphylla
Berberis parodii
Landrum
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00244074_v153_n3_p321_Bottini
https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00244074_v153_n3_p321_Bottini_oai
Aporte de:
Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Sequence analysis of the internal transcribed spacer (ITS) of the 18S(ITS1)-5.8S-26S(ITS2) rDNA region was performed in order to analyse the phylogenetic relationships between 13 Patagonian species of the genus Berberis (Berberidaceae). The divergence values between the pairwise sequence in the studied Patagonian species were in the range 2.9-22.9%. The lengths of the ITS1 and ITS2 sequences were in the range 227-231 bp and 220-224 bp, respectively, and the 5.8S sequence was 159 bp throughout all species. B. microphylla sensu Landrum does not appear to be monophyletic based on current sampling. Indeed, we suggest that B. microphylla should be distinguished from B. buxifolia, B. parodii, and B. heterophylla. ITS sequences, together with data obtained from morphological, biochemical, amplified fragment length polymorphism, and cytological characterizations, support the existence of diploid and polyploid hybrid speciation in the genus. © 2007 The Linnean Society of London.

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