Relationships in Patagonian species of Berberis (Berberidaceae) based on the characterization of rDNA internal transcribed spacer sequences

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Sequence analysis of the internal transcribed spacer (ITS) of the 18S(ITS1)-5.8S-26S(ITS2) rDNA region was performed in order to analyse the phylogenetic relationships between 13 Patagonian species of the genus Berberis (Berberidaceae). The divergence values between the pairwise sequence in the stud...

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Autores principales: Bottini, M.C.J., De Bustos, A., Sanso, A.M., Jouve, N., Poggio, L.
Formato: Artículo publishedVersion
Publicado: 2007
Materias:
Argentina
Barberry
Calafate
Diploid and polyploid hybrid speciation
Michay
Molecular markers
DNA fingerprinting
genetic differentiation
hybridization
molecular analysis
phylogenetics
polymorphism
polyploidy
shrub
speciation (biology)
South America
Berberidaceae
Berberis
Berberis buxifolia
Berberis heterophylla
Berberis microphylla
Berberis parodii
Landrum
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00244074_v153_n3_p321_Bottini
https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00244074_v153_n3_p321_Bottini_oai
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Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires
id I28-R145-paper_00244074_v153_n3_p321_Bottini_oai
record_format dspace
spelling I28-R145-paper_00244074_v153_n3_p321_Bottini_oai2024-08-16 Bottini, M.C.J. De Bustos, A. Sanso, A.M. Jouve, N. Poggio, L. 2007 Sequence analysis of the internal transcribed spacer (ITS) of the 18S(ITS1)-5.8S-26S(ITS2) rDNA region was performed in order to analyse the phylogenetic relationships between 13 Patagonian species of the genus Berberis (Berberidaceae). The divergence values between the pairwise sequence in the studied Patagonian species were in the range 2.9-22.9%. The lengths of the ITS1 and ITS2 sequences were in the range 227-231 bp and 220-224 bp, respectively, and the 5.8S sequence was 159 bp throughout all species. B. microphylla sensu Landrum does not appear to be monophyletic based on current sampling. Indeed, we suggest that B. microphylla should be distinguished from B. buxifolia, B. parodii, and B. heterophylla. ITS sequences, together with data obtained from morphological, biochemical, amplified fragment length polymorphism, and cytological characterizations, support the existence of diploid and polyploid hybrid speciation in the genus. © 2007 The Linnean Society of London. Fil:Bottini, M.C.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Sanso, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Poggio, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_00244074_v153_n3_p321_Bottini info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Bot. J. Linn. Soc. 2007;153(3):321-328 Argentina Barberry Calafate Diploid and polyploid hybrid speciation Michay Molecular markers DNA fingerprinting genetic differentiation hybridization molecular analysis phylogenetics polymorphism polyploidy shrub speciation (biology) Argentina South America Berberidaceae Berberis Berberis buxifolia Berberis heterophylla Berberis microphylla Berberis parodii Landrum Relationships in Patagonian species of Berberis (Berberidaceae) based on the characterization of rDNA internal transcribed spacer sequences info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00244074_v153_n3_p321_Bottini_oai
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-145
collection Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic Argentina
Barberry
Calafate
Diploid and polyploid hybrid speciation
Michay
Molecular markers
DNA fingerprinting
genetic differentiation
hybridization
molecular analysis
phylogenetics
polymorphism
polyploidy
shrub
speciation (biology)
Argentina
South America
Berberidaceae
Berberis
Berberis buxifolia
Berberis heterophylla
Berberis microphylla
Berberis parodii
Landrum
spellingShingle Argentina
Barberry
Calafate
Diploid and polyploid hybrid speciation
Michay
Molecular markers
DNA fingerprinting
genetic differentiation
hybridization
molecular analysis
phylogenetics
polymorphism
polyploidy
shrub
speciation (biology)
Argentina
South America
Berberidaceae
Berberis
Berberis buxifolia
Berberis heterophylla
Berberis microphylla
Berberis parodii
Landrum
Bottini, M.C.J.
De Bustos, A.
Sanso, A.M.
Jouve, N.
Poggio, L.
Relationships in Patagonian species of Berberis (Berberidaceae) based on the characterization of rDNA internal transcribed spacer sequences
topic_facet Argentina
Barberry
Calafate
Diploid and polyploid hybrid speciation
Michay
Molecular markers
DNA fingerprinting
genetic differentiation
hybridization
molecular analysis
phylogenetics
polymorphism
polyploidy
shrub
speciation (biology)
Argentina
South America
Berberidaceae
Berberis
Berberis buxifolia
Berberis heterophylla
Berberis microphylla
Berberis parodii
Landrum
description Sequence analysis of the internal transcribed spacer (ITS) of the 18S(ITS1)-5.8S-26S(ITS2) rDNA region was performed in order to analyse the phylogenetic relationships between 13 Patagonian species of the genus Berberis (Berberidaceae). The divergence values between the pairwise sequence in the studied Patagonian species were in the range 2.9-22.9%. The lengths of the ITS1 and ITS2 sequences were in the range 227-231 bp and 220-224 bp, respectively, and the 5.8S sequence was 159 bp throughout all species. B. microphylla sensu Landrum does not appear to be monophyletic based on current sampling. Indeed, we suggest that B. microphylla should be distinguished from B. buxifolia, B. parodii, and B. heterophylla. ITS sequences, together with data obtained from morphological, biochemical, amplified fragment length polymorphism, and cytological characterizations, support the existence of diploid and polyploid hybrid speciation in the genus. © 2007 The Linnean Society of London.
format Artículo
Artículo
publishedVersion
author Bottini, M.C.J.
De Bustos, A.
Sanso, A.M.
Jouve, N.
Poggio, L.
author_facet Bottini, M.C.J.
De Bustos, A.
Sanso, A.M.
Jouve, N.
Poggio, L.
author_sort Bottini, M.C.J.
title Relationships in Patagonian species of Berberis (Berberidaceae) based on the characterization of rDNA internal transcribed spacer sequences
title_short Relationships in Patagonian species of Berberis (Berberidaceae) based on the characterization of rDNA internal transcribed spacer sequences
title_full Relationships in Patagonian species of Berberis (Berberidaceae) based on the characterization of rDNA internal transcribed spacer sequences
title_fullStr Relationships in Patagonian species of Berberis (Berberidaceae) based on the characterization of rDNA internal transcribed spacer sequences
title_full_unstemmed Relationships in Patagonian species of Berberis (Berberidaceae) based on the characterization of rDNA internal transcribed spacer sequences
title_sort relationships in patagonian species of berberis (berberidaceae) based on the characterization of rdna internal transcribed spacer sequences
publishDate 2007
url http://hdl.handle.net/20.500.12110/paper_00244074_v153_n3_p321_Bottini
https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00244074_v153_n3_p321_Bottini_oai
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