Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B

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Heterogeneous nuclear ribonucleoproteins A and B (hnRNPs A/B), cellular RNA-binding proteins that participate in splicing, trafficking, translation and turnover of mRNAs, have been implicated in the life cycles of several cytoplasmic RNA viruses. Here, we demonstrate that silencing of hnRNPs A1 and...

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Autores principales: Maeto, C.A., Knott, M.E., Linero, F.N., Ellenberg, P.C., Scolaro, L.A., Castilla, V.
Formato: Artículo publishedVersion
Publicado: 2011
Materias:
cell protein
ribonucleoprotein
ribonucleoprotein A1
ribonucleoprotein A2
ribonucleoprotein B
small interfering RNA
unclassified drug
virus nucleoprotein
Argentine hemorrhagic fever
article
cellular distribution
controlled study
gene overexpression
immunoprecipitation
Junin virus
nonhuman
nucleocytoplasmic transport
persistent infection
priority journal
protein expression
protein protein interaction
Vero cell
virus cell interaction
virus replication
Active Transport, Cell Nucleus
Animals
Cercopithecus aethiops
Heterogeneous-Nuclear Ribonucleoprotein Group A-B
Host-Pathogen Interactions
Immunoprecipitation
Protein Binding
RNA, Viral
Vero Cells
Viral Proteins
Virus Replication
Arenavirus
RNA viruses
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00221317_v92_n9_p2181_Maeto
http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00221317_v92_n9_p2181_Maeto_oai
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Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires
id I28-R145-paper_00221317_v92_n9_p2181_Maeto_oai
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spelling I28-R145-paper_00221317_v92_n9_p2181_Maeto_oai2020-10-19 Maeto, C.A. Knott, M.E. Linero, F.N. Ellenberg, P.C. Scolaro, L.A. Castilla, V. 2011 Heterogeneous nuclear ribonucleoproteins A and B (hnRNPs A/B), cellular RNA-binding proteins that participate in splicing, trafficking, translation and turnover of mRNAs, have been implicated in the life cycles of several cytoplasmic RNA viruses. Here, we demonstrate that silencing of hnRNPs A1 and A2 significantly reduces the replication of the arenavirus Juni{dotless} ́n virus (JUNV), the aetiological agent of Argentine haemorrhagic fever. While acute JUNV infection did not modify total levels of expression of hnRNPs A/B in comparison with uninfected cells, non-cytopathic persistent infection exhibited low levels of these cell proteins. Furthermore, acutely infected cells showed a cytoplasmic relocalization of overexpressed hnRNP A1, probably related to the involvement of this protein in virus replicative cycle. This cytoplasmic accumulation was also observed in cells expressing viral nucleoprotein (N), and co-immunoprecipitation studies revealed the interaction between hnRNP A1 and N protein. By contrast, a predominantly nuclear distribution of overexpressed hnRNP A1 was found during persistent infection, even in the presence of endogenous or overexpressed N protein, indicating a differential modulation of nucleo-cytoplasmic trafficking in acute and persistent JUNV infections. © 2011 SGM. Fil:Maeto, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Knott, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Linero, F.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ellenberg, P.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Scolaro, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Castilla, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_00221317_v92_n9_p2181_Maeto info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar J. Gen. Virol. 2011;92(9):2181-2190 cell protein ribonucleoprotein ribonucleoprotein A1 ribonucleoprotein A2 ribonucleoprotein B small interfering RNA unclassified drug virus nucleoprotein Argentine hemorrhagic fever article cellular distribution controlled study gene overexpression immunoprecipitation Junin virus nonhuman nucleocytoplasmic transport persistent infection priority journal protein expression protein protein interaction Vero cell virus cell interaction virus replication Active Transport, Cell Nucleus Animals Cercopithecus aethiops Heterogeneous-Nuclear Ribonucleoprotein Group A-B Host-Pathogen Interactions Immunoprecipitation Junin virus Protein Binding RNA, Viral Vero Cells Viral Proteins Virus Replication Arenavirus Junin virus RNA viruses Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00221317_v92_n9_p2181_Maeto_oai
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-145
collection Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic cell protein
ribonucleoprotein
ribonucleoprotein A1
ribonucleoprotein A2
ribonucleoprotein B
small interfering RNA
unclassified drug
virus nucleoprotein
Argentine hemorrhagic fever
article
cellular distribution
controlled study
gene overexpression
immunoprecipitation
Junin virus
nonhuman
nucleocytoplasmic transport
persistent infection
priority journal
protein expression
protein protein interaction
Vero cell
virus cell interaction
virus replication
Active Transport, Cell Nucleus
Animals
Cercopithecus aethiops
Heterogeneous-Nuclear Ribonucleoprotein Group A-B
Host-Pathogen Interactions
Immunoprecipitation
Junin virus
Protein Binding
RNA, Viral
Vero Cells
Viral Proteins
Virus Replication
Arenavirus
Junin virus
RNA viruses
spellingShingle cell protein
ribonucleoprotein
ribonucleoprotein A1
ribonucleoprotein A2
ribonucleoprotein B
small interfering RNA
unclassified drug
virus nucleoprotein
Argentine hemorrhagic fever
article
cellular distribution
controlled study
gene overexpression
immunoprecipitation
Junin virus
nonhuman
nucleocytoplasmic transport
persistent infection
priority journal
protein expression
protein protein interaction
Vero cell
virus cell interaction
virus replication
Active Transport, Cell Nucleus
Animals
Cercopithecus aethiops
Heterogeneous-Nuclear Ribonucleoprotein Group A-B
Host-Pathogen Interactions
Immunoprecipitation
Junin virus
Protein Binding
RNA, Viral
Vero Cells
Viral Proteins
Virus Replication
Arenavirus
Junin virus
RNA viruses
Maeto, C.A.
Knott, M.E.
Linero, F.N.
Ellenberg, P.C.
Scolaro, L.A.
Castilla, V.
Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B
topic_facet cell protein
ribonucleoprotein
ribonucleoprotein A1
ribonucleoprotein A2
ribonucleoprotein B
small interfering RNA
unclassified drug
virus nucleoprotein
Argentine hemorrhagic fever
article
cellular distribution
controlled study
gene overexpression
immunoprecipitation
Junin virus
nonhuman
nucleocytoplasmic transport
persistent infection
priority journal
protein expression
protein protein interaction
Vero cell
virus cell interaction
virus replication
Active Transport, Cell Nucleus
Animals
Cercopithecus aethiops
Heterogeneous-Nuclear Ribonucleoprotein Group A-B
Host-Pathogen Interactions
Immunoprecipitation
Junin virus
Protein Binding
RNA, Viral
Vero Cells
Viral Proteins
Virus Replication
Arenavirus
Junin virus
RNA viruses
description Heterogeneous nuclear ribonucleoproteins A and B (hnRNPs A/B), cellular RNA-binding proteins that participate in splicing, trafficking, translation and turnover of mRNAs, have been implicated in the life cycles of several cytoplasmic RNA viruses. Here, we demonstrate that silencing of hnRNPs A1 and A2 significantly reduces the replication of the arenavirus Juni{dotless} ́n virus (JUNV), the aetiological agent of Argentine haemorrhagic fever. While acute JUNV infection did not modify total levels of expression of hnRNPs A/B in comparison with uninfected cells, non-cytopathic persistent infection exhibited low levels of these cell proteins. Furthermore, acutely infected cells showed a cytoplasmic relocalization of overexpressed hnRNP A1, probably related to the involvement of this protein in virus replicative cycle. This cytoplasmic accumulation was also observed in cells expressing viral nucleoprotein (N), and co-immunoprecipitation studies revealed the interaction between hnRNP A1 and N protein. By contrast, a predominantly nuclear distribution of overexpressed hnRNP A1 was found during persistent infection, even in the presence of endogenous or overexpressed N protein, indicating a differential modulation of nucleo-cytoplasmic trafficking in acute and persistent JUNV infections. © 2011 SGM.
format Artículo
Artículo
publishedVersion
author Maeto, C.A.
Knott, M.E.
Linero, F.N.
Ellenberg, P.C.
Scolaro, L.A.
Castilla, V.
author_facet Maeto, C.A.
Knott, M.E.
Linero, F.N.
Ellenberg, P.C.
Scolaro, L.A.
Castilla, V.
author_sort Maeto, C.A.
title Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B
title_short Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B
title_full Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B
title_fullStr Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B
title_full_unstemmed Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B
title_sort differential effect of acute and persistent junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type a and b
publishDate 2011
url http://hdl.handle.net/20.500.12110/paper_00221317_v92_n9_p2181_Maeto
http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00221317_v92_n9_p2181_Maeto_oai
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AT knottme differentialeffectofacuteandpersistentjuninvirusinfectionsonthenucleocytoplasmictraffickingandexpressionofheterogeneousnuclearribonucleoproteinstypeaandb
AT linerofn differentialeffectofacuteandpersistentjuninvirusinfectionsonthenucleocytoplasmictraffickingandexpressionofheterogeneousnuclearribonucleoproteinstypeaandb
AT ellenbergpc differentialeffectofacuteandpersistentjuninvirusinfectionsonthenucleocytoplasmictraffickingandexpressionofheterogeneousnuclearribonucleoproteinstypeaandb
AT scolarola differentialeffectofacuteandpersistentjuninvirusinfectionsonthenucleocytoplasmictraffickingandexpressionofheterogeneousnuclearribonucleoproteinstypeaandb
AT castillav differentialeffectofacuteandpersistentjuninvirusinfectionsonthenucleocytoplasmictraffickingandexpressionofheterogeneousnuclearribonucleoproteinstypeaandb
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