Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp.

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Ethylene inhibits the establishment of symbiosis between rhizobia and legumes. Several rhizobia species express the enzyme ACC deaminase, which degrades the ethylene precursor 1-cyclopropane-1-carboxilate (ACC), leading to reductions in the amount of ethylene evolved by the plant. M. loti has a gene...

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Autores principales:	Conforte, V.P., Echeverria, M., Sánchez, C., Ugalde, R.A., Menéndez, A.B., Lepek, V.C.
Formato:	Artículo publishedVersion
Publicado:	2010
Materias:	ACC deaminase Competitiveness Ethylene Lotus Mesorhizobium loti 1 aminocyclopropanecarboxylic acid 1 aminocyclopropanecarboxylic acid deaminase deaminase ethylene unclassified drug 1-aminocyclopropane-1-carboxylate deaminase lyase article bacterial cell bacterium competence controlled study enzyme activity enzyme degradation forage genetic engineering legume nodulation nonhuman plant chromosome promoter region protein expression Rhizobiaceae strain difference structural gene symbiosis wild type Alphaproteobacteria enzymology genetics microbiology plant root Lotus corniculatus var. japonicus Lotus tenuis Carbon-Carbon Lyases Plant Roots Symbiosis
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00221260_v56_n4_p331_Conforte http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00221260_v56_n4_p331_Conforte_oai
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

id	I28-R145-paper_00221260_v56_n4_p331_Conforte_oai
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spelling	I28-R145-paper_00221260_v56_n4_p331_Conforte_oai2020-10-19 Conforte, V.P. Echeverria, M. Sánchez, C. Ugalde, R.A. Menéndez, A.B. Lepek, V.C. 2010 Ethylene inhibits the establishment of symbiosis between rhizobia and legumes. Several rhizobia species express the enzyme ACC deaminase, which degrades the ethylene precursor 1-cyclopropane-1-carboxilate (ACC), leading to reductions in the amount of ethylene evolved by the plant. M. loti has a gene encoding ACC deaminase, but this gene is under the activity of the NifA-RpoN- dependent promoter; thus, it is only expressed inside the nodule. The M. loti structural gene ACC deaminase (acdS) was integrated into the M. loti chromosome under a constitutive promoter activity. The resulting strain induced the formation of a higher number of nodules and was more competitive than the wild-type strain on Lotus japonicus and L. tenuis. These results suggest that the introduction of the ACC deaminase activity within M. loti in a constitutive way could be a novel strategy to increase nodulation competitiveness of the bacteria, which could be useful for the forage inoculants industry. Fil:Conforte, V.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Echeverria, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ugalde, R.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Menéndez, A.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lepek, V.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_00221260_v56_n4_p331_Conforte info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar J. Gen. Appl. Microbiol. 2010;56(4):331-338 ACC deaminase Competitiveness Ethylene Lotus Mesorhizobium loti 1 aminocyclopropanecarboxylic acid 1 aminocyclopropanecarboxylic acid deaminase deaminase ethylene unclassified drug 1-aminocyclopropane-1-carboxylate deaminase lyase article bacterial cell bacterium competence controlled study enzyme activity enzyme degradation forage genetic engineering legume Lotus Mesorhizobium loti nodulation nonhuman plant chromosome promoter region protein expression Rhizobiaceae strain difference structural gene symbiosis wild type Alphaproteobacteria enzymology genetics microbiology plant root symbiosis Lotus corniculatus var. japonicus Lotus tenuis Mesorhizobium loti Alphaproteobacteria Carbon-Carbon Lyases Lotus Plant Roots Symbiosis Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp. info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00221260_v56_n4_p331_Conforte_oai
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic	ACC deaminase Competitiveness Ethylene Lotus Mesorhizobium loti 1 aminocyclopropanecarboxylic acid 1 aminocyclopropanecarboxylic acid deaminase deaminase ethylene unclassified drug 1-aminocyclopropane-1-carboxylate deaminase lyase article bacterial cell bacterium competence controlled study enzyme activity enzyme degradation forage genetic engineering legume Lotus Mesorhizobium loti nodulation nonhuman plant chromosome promoter region protein expression Rhizobiaceae strain difference structural gene symbiosis wild type Alphaproteobacteria enzymology genetics microbiology plant root symbiosis Lotus corniculatus var. japonicus Lotus tenuis Mesorhizobium loti Alphaproteobacteria Carbon-Carbon Lyases Lotus Plant Roots Symbiosis
spellingShingle	ACC deaminase Competitiveness Ethylene Lotus Mesorhizobium loti 1 aminocyclopropanecarboxylic acid 1 aminocyclopropanecarboxylic acid deaminase deaminase ethylene unclassified drug 1-aminocyclopropane-1-carboxylate deaminase lyase article bacterial cell bacterium competence controlled study enzyme activity enzyme degradation forage genetic engineering legume Lotus Mesorhizobium loti nodulation nonhuman plant chromosome promoter region protein expression Rhizobiaceae strain difference structural gene symbiosis wild type Alphaproteobacteria enzymology genetics microbiology plant root symbiosis Lotus corniculatus var. japonicus Lotus tenuis Mesorhizobium loti Alphaproteobacteria Carbon-Carbon Lyases Lotus Plant Roots Symbiosis Conforte, V.P. Echeverria, M. Sánchez, C. Ugalde, R.A. Menéndez, A.B. Lepek, V.C. Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp.
topic_facet	ACC deaminase Competitiveness Ethylene Lotus Mesorhizobium loti 1 aminocyclopropanecarboxylic acid 1 aminocyclopropanecarboxylic acid deaminase deaminase ethylene unclassified drug 1-aminocyclopropane-1-carboxylate deaminase lyase article bacterial cell bacterium competence controlled study enzyme activity enzyme degradation forage genetic engineering legume Lotus Mesorhizobium loti nodulation nonhuman plant chromosome promoter region protein expression Rhizobiaceae strain difference structural gene symbiosis wild type Alphaproteobacteria enzymology genetics microbiology plant root symbiosis Lotus corniculatus var. japonicus Lotus tenuis Mesorhizobium loti Alphaproteobacteria Carbon-Carbon Lyases Lotus Plant Roots Symbiosis
description	Ethylene inhibits the establishment of symbiosis between rhizobia and legumes. Several rhizobia species express the enzyme ACC deaminase, which degrades the ethylene precursor 1-cyclopropane-1-carboxilate (ACC), leading to reductions in the amount of ethylene evolved by the plant. M. loti has a gene encoding ACC deaminase, but this gene is under the activity of the NifA-RpoN- dependent promoter; thus, it is only expressed inside the nodule. The M. loti structural gene ACC deaminase (acdS) was integrated into the M. loti chromosome under a constitutive promoter activity. The resulting strain induced the formation of a higher number of nodules and was more competitive than the wild-type strain on Lotus japonicus and L. tenuis. These results suggest that the introduction of the ACC deaminase activity within M. loti in a constitutive way could be a novel strategy to increase nodulation competitiveness of the bacteria, which could be useful for the forage inoculants industry.
format	Artículo Artículo publishedVersion
author	Conforte, V.P. Echeverria, M. Sánchez, C. Ugalde, R.A. Menéndez, A.B. Lepek, V.C.
author_facet	Conforte, V.P. Echeverria, M. Sánchez, C. Ugalde, R.A. Menéndez, A.B. Lepek, V.C.
author_sort	Conforte, V.P.
title	Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp.
title_short	Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp.
title_full	Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp.
title_fullStr	Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp.
title_full_unstemmed	Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp.
title_sort	engineered acc deaminase-expressing free-living cells of mesorhizobium loti show increased nodulation efficiency and competitiveness on lotus spp.
publishDate	2010
url	http://hdl.handle.net/20.500.12110/paper_00221260_v56_n4_p331_Conforte http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00221260_v56_n4_p331_Conforte_oai
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_version_	1766026585268289536

Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp.

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