Acetoacetyl-CoA thiolase regulates the mevalonate pathway during abiotic stress adaptation
Acetoacetyl-CoA thiolase (EC 2.3.1.9), also called thiolase II, condenses two molecules of acetyl-CoA to give acetoacetyl-CoA. This is the first enzymatic step in the biosynthesis of isoprenoids via mevalonate (MVA). In this work, thiolase II from alfalfa (MsAACT1) was identified and cloned. The enz...
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| Formato: | Artículo publishedVersion |
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2011
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00220957_v62_n15_p5699_Soto http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00220957_v62_n15_p5699_Soto_oai |
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I28-R145-paper_00220957_v62_n15_p5699_Soto_oai2020-10-19 Soto, G. Stritzler, M. Lisi, C. Alleva, K. Pagano, M.E. Ardila, F. Mozzicafreddo, M. Cuccioloni, M. Angeletti, M. Ayub, N.D. 2011 Acetoacetyl-CoA thiolase (EC 2.3.1.9), also called thiolase II, condenses two molecules of acetyl-CoA to give acetoacetyl-CoA. This is the first enzymatic step in the biosynthesis of isoprenoids via mevalonate (MVA). In this work, thiolase II from alfalfa (MsAACT1) was identified and cloned. The enzymatic activity was experimentally demonstrated in planta and in heterologous systems. The condensation reaction by MsAACT1 was proved to be inhibited by CoA suggesting a negative feedback regulation of isoprenoid production. Real-time RT-PCR analysis indicated that MsAACT1 expression is highly increased in roots and leaves under cold and salinity stress. Treatment with mevastatin, a specific inhibitor of the MVA pathway, resulted in a decrease in squalene production, antioxidant activity, and the survival of stressed plants. As expected, the presence of mevastatin did not change chlorophyll and carotenoid levels, isoprenoids synthesized via the plastidial MVA-independent pathway. The addition of vitamin C suppressed the sensitive phenotype of plants challenged with mevastatin, suggesting a critical function of the MVA pathway in abiotic stress-inducible antioxidant defence. MsAACT1 over-expressing transgenic plants showed salinity tolerance comparable with empty vector transformed plants and enhanced production of squalene without altering the 3-hydroxy-3-methylglutaryl- CoA reductase (HMGR) activity in salt-stress conditions. Thus, acetoacetyl-CoA thiolase is a regulatory enzyme in isoprenoid biosynthesis involved in abiotic stress adaptation. © 2011 The Author. Fil:Soto, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Stritzler, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ardila, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ayub, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_00220957_v62_n15_p5699_Soto info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar J. Exp. Bot. 2011;62(15):5699-5711 Abiotic stress acetoacetyl-CoA thiolase isoprenoid MVA pathway thiolase II acetyl coenzyme A acetyltransferase mevalonic acid pravastatin squalene vegetable protein alfalfa article drug effect genetics metabolism plant leaf plant root real time polymerase chain reaction signal transduction transgenic plant Acetyl-CoA C-Acetyltransferase Medicago sativa Mevalonic Acid Plant Leaves Plant Proteins Plant Roots Plants, Genetically Modified Pravastatin Real-Time Polymerase Chain Reaction Signal Transduction Squalene Acetoacetyl-CoA thiolase regulates the mevalonate pathway during abiotic stress adaptation info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00220957_v62_n15_p5699_Soto_oai |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| topic |
Abiotic stress acetoacetyl-CoA thiolase isoprenoid MVA pathway thiolase II acetyl coenzyme A acetyltransferase mevalonic acid pravastatin squalene vegetable protein alfalfa article drug effect genetics metabolism plant leaf plant root real time polymerase chain reaction signal transduction transgenic plant Acetyl-CoA C-Acetyltransferase Medicago sativa Mevalonic Acid Plant Leaves Plant Proteins Plant Roots Plants, Genetically Modified Pravastatin Real-Time Polymerase Chain Reaction Signal Transduction Squalene |
| spellingShingle |
Abiotic stress acetoacetyl-CoA thiolase isoprenoid MVA pathway thiolase II acetyl coenzyme A acetyltransferase mevalonic acid pravastatin squalene vegetable protein alfalfa article drug effect genetics metabolism plant leaf plant root real time polymerase chain reaction signal transduction transgenic plant Acetyl-CoA C-Acetyltransferase Medicago sativa Mevalonic Acid Plant Leaves Plant Proteins Plant Roots Plants, Genetically Modified Pravastatin Real-Time Polymerase Chain Reaction Signal Transduction Squalene Soto, G. Stritzler, M. Lisi, C. Alleva, K. Pagano, M.E. Ardila, F. Mozzicafreddo, M. Cuccioloni, M. Angeletti, M. Ayub, N.D. Acetoacetyl-CoA thiolase regulates the mevalonate pathway during abiotic stress adaptation |
| topic_facet |
Abiotic stress acetoacetyl-CoA thiolase isoprenoid MVA pathway thiolase II acetyl coenzyme A acetyltransferase mevalonic acid pravastatin squalene vegetable protein alfalfa article drug effect genetics metabolism plant leaf plant root real time polymerase chain reaction signal transduction transgenic plant Acetyl-CoA C-Acetyltransferase Medicago sativa Mevalonic Acid Plant Leaves Plant Proteins Plant Roots Plants, Genetically Modified Pravastatin Real-Time Polymerase Chain Reaction Signal Transduction Squalene |
| description |
Acetoacetyl-CoA thiolase (EC 2.3.1.9), also called thiolase II, condenses two molecules of acetyl-CoA to give acetoacetyl-CoA. This is the first enzymatic step in the biosynthesis of isoprenoids via mevalonate (MVA). In this work, thiolase II from alfalfa (MsAACT1) was identified and cloned. The enzymatic activity was experimentally demonstrated in planta and in heterologous systems. The condensation reaction by MsAACT1 was proved to be inhibited by CoA suggesting a negative feedback regulation of isoprenoid production. Real-time RT-PCR analysis indicated that MsAACT1 expression is highly increased in roots and leaves under cold and salinity stress. Treatment with mevastatin, a specific inhibitor of the MVA pathway, resulted in a decrease in squalene production, antioxidant activity, and the survival of stressed plants. As expected, the presence of mevastatin did not change chlorophyll and carotenoid levels, isoprenoids synthesized via the plastidial MVA-independent pathway. The addition of vitamin C suppressed the sensitive phenotype of plants challenged with mevastatin, suggesting a critical function of the MVA pathway in abiotic stress-inducible antioxidant defence. MsAACT1 over-expressing transgenic plants showed salinity tolerance comparable with empty vector transformed plants and enhanced production of squalene without altering the 3-hydroxy-3-methylglutaryl- CoA reductase (HMGR) activity in salt-stress conditions. Thus, acetoacetyl-CoA thiolase is a regulatory enzyme in isoprenoid biosynthesis involved in abiotic stress adaptation. © 2011 The Author. |
| format |
Artículo Artículo publishedVersion |
| author |
Soto, G. Stritzler, M. Lisi, C. Alleva, K. Pagano, M.E. Ardila, F. Mozzicafreddo, M. Cuccioloni, M. Angeletti, M. Ayub, N.D. |
| author_facet |
Soto, G. Stritzler, M. Lisi, C. Alleva, K. Pagano, M.E. Ardila, F. Mozzicafreddo, M. Cuccioloni, M. Angeletti, M. Ayub, N.D. |
| author_sort |
Soto, G. |
| title |
Acetoacetyl-CoA thiolase regulates the mevalonate pathway during abiotic stress adaptation |
| title_short |
Acetoacetyl-CoA thiolase regulates the mevalonate pathway during abiotic stress adaptation |
| title_full |
Acetoacetyl-CoA thiolase regulates the mevalonate pathway during abiotic stress adaptation |
| title_fullStr |
Acetoacetyl-CoA thiolase regulates the mevalonate pathway during abiotic stress adaptation |
| title_full_unstemmed |
Acetoacetyl-CoA thiolase regulates the mevalonate pathway during abiotic stress adaptation |
| title_sort |
acetoacetyl-coa thiolase regulates the mevalonate pathway during abiotic stress adaptation |
| publishDate |
2011 |
| url |
http://hdl.handle.net/20.500.12110/paper_00220957_v62_n15_p5699_Soto http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00220957_v62_n15_p5699_Soto_oai |
| work_keys_str_mv |
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| _version_ |
1766026583598956544 |