Transepithelial potential differences and Na+ flux in isolated perfused gills of the crab Chasmagnathus granulatus (Grapsidae) acclimated to hyper- and hypo-salinity
We studied the transepithelial potential difference (TEPD) and 22Na flux across isolated perfused gills (anterior pair 5 and posterior pairs 6-8) of the crab Chasmagnathus granulatus acclimated to either hypo- or hyper-osmotic conditions. The gills of crabs acclimated to low salinity, perfused and b...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | Artículo publishedVersion |
| Publicado: |
2002
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00220949_v205_n1_p71_Luquet https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00220949_v205_n1_p71_Luquet_oai |
| Aporte de: |
| id |
I28-R145-paper_00220949_v205_n1_p71_Luquet_oai |
|---|---|
| record_format |
dspace |
| spelling |
I28-R145-paper_00220949_v205_n1_p71_Luquet_oai2024-08-16 Luquet, C.M. Postel, U. Halperin, J. Urcola, M.R. Marques, R. Siebers, D. 2002 We studied the transepithelial potential difference (TEPD) and 22Na flux across isolated perfused gills (anterior pair 5 and posterior pairs 6-8) of the crab Chasmagnathus granulatus acclimated to either hypo- or hyper-osmotic conditions. The gills of crabs acclimated to low salinity, perfused and bathed with 10%‰ saline solutions, produced the following TEPDs (hemolymph side with respect to bath side): 0.4±0.7, -10.2±1.6, -10.8±1.3 and -6.7±1.3mV for gills 5, 6, 7 and 8, respectively. Gills 6, 7 and 8 did not differ significantly. Reducing the saline concentration of bath and perfusate from 30%‰ to 20%‰ or 10%‰ increased significantly the TEPDs of these gills. TEPDs of gill 6 (representative of posterior gills) were reduced by 69±5% and 60±5% after perfusion with ouabain or BaCl2 (5mmoll-1 each), respectively. The same gill showed a net ouabain-sensitive Na+ influx of 1150±290 μequiv g-1h-1. Gill 6 of crabs acclimated to high salinity produced TEPDs of -1.5±0.1 and -1.3±0.09mV after perfusion with 30%‰ or 40%‰ salines, respectively. Perfusion with ouabain or BaCl2 reduced TEPDs by 76±7% and 86±4%, respectively. A net ouabain-sensitive Na+ efflux of 2282±337 μequiv g-1h-1 was recorded in gill 6 perfused with 38%‰ saline. Fil:Luquet, C.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Halperin, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Marques, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_00220949_v205_n1_p71_Luquet info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar J. Exp. Biol. 2002;205(1):71-77 Chasmagnathus granulatus Crab Hyper-regulation Hypo-regulation Ion flux Isolated perfused gill Na+K+-ATPase Ouabain Transepithelial potential difference adenosine triphosphatase (potassium sodium) hypotonic solution sodium sodium chloride adaptation animal Argentina article Brachyura cell membrane potential epithelium gill metabolism physiology Adaptation, Physiological Animals Argentina Brachyura Epithelium Gills Hypotonic Solutions Membrane Potentials Na(+)-K(+)-Exchanging ATPase Saline Solution, Hypertonic Sodium Sodium Chloride Animalia Brachyura Chasmagnathus granulata Decapoda (Crustacea) Grapsidae Strophanthus gratus Transepithelial potential differences and Na+ flux in isolated perfused gills of the crab Chasmagnathus granulatus (Grapsidae) acclimated to hyper- and hypo-salinity info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00220949_v205_n1_p71_Luquet_oai |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| topic |
Chasmagnathus granulatus Crab Hyper-regulation Hypo-regulation Ion flux Isolated perfused gill Na+K+-ATPase Ouabain Transepithelial potential difference adenosine triphosphatase (potassium sodium) hypotonic solution sodium sodium chloride adaptation animal Argentina article Brachyura cell membrane potential epithelium gill metabolism physiology Adaptation, Physiological Animals Argentina Brachyura Epithelium Gills Hypotonic Solutions Membrane Potentials Na(+)-K(+)-Exchanging ATPase Saline Solution, Hypertonic Sodium Sodium Chloride Animalia Brachyura Chasmagnathus granulata Decapoda (Crustacea) Grapsidae Strophanthus gratus |
| spellingShingle |
Chasmagnathus granulatus Crab Hyper-regulation Hypo-regulation Ion flux Isolated perfused gill Na+K+-ATPase Ouabain Transepithelial potential difference adenosine triphosphatase (potassium sodium) hypotonic solution sodium sodium chloride adaptation animal Argentina article Brachyura cell membrane potential epithelium gill metabolism physiology Adaptation, Physiological Animals Argentina Brachyura Epithelium Gills Hypotonic Solutions Membrane Potentials Na(+)-K(+)-Exchanging ATPase Saline Solution, Hypertonic Sodium Sodium Chloride Animalia Brachyura Chasmagnathus granulata Decapoda (Crustacea) Grapsidae Strophanthus gratus Luquet, C.M. Postel, U. Halperin, J. Urcola, M.R. Marques, R. Siebers, D. Transepithelial potential differences and Na+ flux in isolated perfused gills of the crab Chasmagnathus granulatus (Grapsidae) acclimated to hyper- and hypo-salinity |
| topic_facet |
Chasmagnathus granulatus Crab Hyper-regulation Hypo-regulation Ion flux Isolated perfused gill Na+K+-ATPase Ouabain Transepithelial potential difference adenosine triphosphatase (potassium sodium) hypotonic solution sodium sodium chloride adaptation animal Argentina article Brachyura cell membrane potential epithelium gill metabolism physiology Adaptation, Physiological Animals Argentina Brachyura Epithelium Gills Hypotonic Solutions Membrane Potentials Na(+)-K(+)-Exchanging ATPase Saline Solution, Hypertonic Sodium Sodium Chloride Animalia Brachyura Chasmagnathus granulata Decapoda (Crustacea) Grapsidae Strophanthus gratus |
| description |
We studied the transepithelial potential difference (TEPD) and 22Na flux across isolated perfused gills (anterior pair 5 and posterior pairs 6-8) of the crab Chasmagnathus granulatus acclimated to either hypo- or hyper-osmotic conditions. The gills of crabs acclimated to low salinity, perfused and bathed with 10%‰ saline solutions, produced the following TEPDs (hemolymph side with respect to bath side): 0.4±0.7, -10.2±1.6, -10.8±1.3 and -6.7±1.3mV for gills 5, 6, 7 and 8, respectively. Gills 6, 7 and 8 did not differ significantly. Reducing the saline concentration of bath and perfusate from 30%‰ to 20%‰ or 10%‰ increased significantly the TEPDs of these gills. TEPDs of gill 6 (representative of posterior gills) were reduced by 69±5% and 60±5% after perfusion with ouabain or BaCl2 (5mmoll-1 each), respectively. The same gill showed a net ouabain-sensitive Na+ influx of 1150±290 μequiv g-1h-1. Gill 6 of crabs acclimated to high salinity produced TEPDs of -1.5±0.1 and -1.3±0.09mV after perfusion with 30%‰ or 40%‰ salines, respectively. Perfusion with ouabain or BaCl2 reduced TEPDs by 76±7% and 86±4%, respectively. A net ouabain-sensitive Na+ efflux of 2282±337 μequiv g-1h-1 was recorded in gill 6 perfused with 38%‰ saline. |
| format |
Artículo Artículo publishedVersion |
| author |
Luquet, C.M. Postel, U. Halperin, J. Urcola, M.R. Marques, R. Siebers, D. |
| author_facet |
Luquet, C.M. Postel, U. Halperin, J. Urcola, M.R. Marques, R. Siebers, D. |
| author_sort |
Luquet, C.M. |
| title |
Transepithelial potential differences and Na+ flux in isolated perfused gills of the crab Chasmagnathus granulatus (Grapsidae) acclimated to hyper- and hypo-salinity |
| title_short |
Transepithelial potential differences and Na+ flux in isolated perfused gills of the crab Chasmagnathus granulatus (Grapsidae) acclimated to hyper- and hypo-salinity |
| title_full |
Transepithelial potential differences and Na+ flux in isolated perfused gills of the crab Chasmagnathus granulatus (Grapsidae) acclimated to hyper- and hypo-salinity |
| title_fullStr |
Transepithelial potential differences and Na+ flux in isolated perfused gills of the crab Chasmagnathus granulatus (Grapsidae) acclimated to hyper- and hypo-salinity |
| title_full_unstemmed |
Transepithelial potential differences and Na+ flux in isolated perfused gills of the crab Chasmagnathus granulatus (Grapsidae) acclimated to hyper- and hypo-salinity |
| title_sort |
transepithelial potential differences and na+ flux in isolated perfused gills of the crab chasmagnathus granulatus (grapsidae) acclimated to hyper- and hypo-salinity |
| publishDate |
2002 |
| url |
http://hdl.handle.net/20.500.12110/paper_00220949_v205_n1_p71_Luquet https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00220949_v205_n1_p71_Luquet_oai |
| work_keys_str_mv |
AT luquetcm transepithelialpotentialdifferencesandnafluxinisolatedperfusedgillsofthecrabchasmagnathusgranulatusgrapsidaeacclimatedtohyperandhyposalinity AT postelu transepithelialpotentialdifferencesandnafluxinisolatedperfusedgillsofthecrabchasmagnathusgranulatusgrapsidaeacclimatedtohyperandhyposalinity AT halperinj transepithelialpotentialdifferencesandnafluxinisolatedperfusedgillsofthecrabchasmagnathusgranulatusgrapsidaeacclimatedtohyperandhyposalinity AT urcolamr transepithelialpotentialdifferencesandnafluxinisolatedperfusedgillsofthecrabchasmagnathusgranulatusgrapsidaeacclimatedtohyperandhyposalinity AT marquesr transepithelialpotentialdifferencesandnafluxinisolatedperfusedgillsofthecrabchasmagnathusgranulatusgrapsidaeacclimatedtohyperandhyposalinity AT siebersd transepithelialpotentialdifferencesandnafluxinisolatedperfusedgillsofthecrabchasmagnathusgranulatusgrapsidaeacclimatedtohyperandhyposalinity |
| _version_ |
1809356786778505216 |