Leptin modulates the expression of its receptors in the hypothalamic-pituitary - Ovarian axis in a differential way

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To investigate the expression of leptin receptors (Ob-R) in the rat hypothalamus-pituitary-ovarian axis, immature rats were treated with eCG/ hCG and Ob-R expression was evaluated by western blot analysis. The Ob-R expression increased 24 h after eCG administration in all the tissues assayed. In the...

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Autores principales: Di Yorio, M.P., Bilbao, M.G., Pustovrh, M.C., Prestifilippo, J.P., Faletti, A.G.
Formato: Artículo publishedVersion
Publicado: 2008
Materias:
gonadorelin
leptin
leptin receptor
progesterone
animal experiment
animal tissue
article
female
hormone synthesis
hypothalamus hypophysis ovarian axis
hypothalamus hypophysis system
immunohistochemistry
nonhuman
ovulation
priority journal
process control
protein content
protein expression
radioimmunoassay
rat
tissue level
Western blotting
Animals
Blotting, Western
Chorionic Gonadotropin
Female
Gene Expression
Gonadotropin-Releasing Hormone
Hypothalamus
Immunohistochemistry
Leptin
Ovary
Pituitary Gland
Rats
Rats, Sprague-Dawley
Receptors, Leptin
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00220795_v198_n2_p355_DiYorio
https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00220795_v198_n2_p355_DiYorio_oai
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Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires
id I28-R145-paper_00220795_v198_n2_p355_DiYorio_oai
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spelling I28-R145-paper_00220795_v198_n2_p355_DiYorio_oai2024-08-16 Di Yorio, M.P. Bilbao, M.G. Pustovrh, M.C. Prestifilippo, J.P. Faletti, A.G. 2008 To investigate the expression of leptin receptors (Ob-R) in the rat hypothalamus-pituitary-ovarian axis, immature rats were treated with eCG/ hCG and Ob-R expression was evaluated by western blot analysis. The Ob-R expression increased 24 h after eCG administration in all the tissues assayed. In the hypothalamus, these levels immediately decreased to those obtained without treatment. In the pituitary, the Ob-R expression continued to be elevated 48 h after eCG administration, whereas the hCG injection did not modify these levels. Similar results were obtained with the ovarian long isoform. To assess the effect of leptin on its receptors, Ob-R was assessed in hypothalamus, pituitary and ovarian explants cultured in the presence or absence of leptin (0.3-500 ng/ml). In the hypothalamus, we found a biphasic effect: the Ob-R expression was either reduced or increased at low or high concentrations of leptin respectively. LH-releasing hormone secretion increased at 1 ng/ml. In the pituitary, Ob-R increased at 10 or 30 ng/ml of leptin for the long and short isoforms respectively. Leptin also induced an increase in LH release at 30 ng/ml. In the ovarian culture, the presence of leptin produced an increase in Ob-R expression at different ranges of concentrations and a dose-dependent biphasic effect on the progesterone, production. In conclusion, all these results clearly suggest that leptin is able to modulate the expression of its own receptors in the reproductive axis in a differential way. Moreover, the positive or negative effect that leptin exerts on the ovulatory process may be dependent on this regulation. © 2008 Society for Endocrinology. Fil:Di Yorio, M.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bilbao, M.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pustovrh, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Faletti, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_00220795_v198_n2_p355_DiYorio info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar J. Endocrinol. 2008;198(2):355-366 gonadorelin leptin leptin receptor progesterone animal experiment animal tissue article female hormone synthesis hypothalamus hypophysis ovarian axis hypothalamus hypophysis system immunohistochemistry nonhuman ovulation priority journal process control protein content protein expression radioimmunoassay rat tissue level Western blotting Animals Blotting, Western Chorionic Gonadotropin Female Gene Expression Gonadotropin-Releasing Hormone Hypothalamus Immunohistochemistry Leptin Ovary Pituitary Gland Rats Rats, Sprague-Dawley Receptors, Leptin Leptin modulates the expression of its receptors in the hypothalamic-pituitary - Ovarian axis in a differential way info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00220795_v198_n2_p355_DiYorio_oai
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-145
collection Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic gonadorelin
leptin
leptin receptor
progesterone
animal experiment
animal tissue
article
female
hormone synthesis
hypothalamus hypophysis ovarian axis
hypothalamus hypophysis system
immunohistochemistry
nonhuman
ovulation
priority journal
process control
protein content
protein expression
radioimmunoassay
rat
tissue level
Western blotting
Animals
Blotting, Western
Chorionic Gonadotropin
Female
Gene Expression
Gonadotropin-Releasing Hormone
Hypothalamus
Immunohistochemistry
Leptin
Ovary
Pituitary Gland
Rats
Rats, Sprague-Dawley
Receptors, Leptin
spellingShingle gonadorelin
leptin
leptin receptor
progesterone
animal experiment
animal tissue
article
female
hormone synthesis
hypothalamus hypophysis ovarian axis
hypothalamus hypophysis system
immunohistochemistry
nonhuman
ovulation
priority journal
process control
protein content
protein expression
radioimmunoassay
rat
tissue level
Western blotting
Animals
Blotting, Western
Chorionic Gonadotropin
Female
Gene Expression
Gonadotropin-Releasing Hormone
Hypothalamus
Immunohistochemistry
Leptin
Ovary
Pituitary Gland
Rats
Rats, Sprague-Dawley
Receptors, Leptin
Di Yorio, M.P.
Bilbao, M.G.
Pustovrh, M.C.
Prestifilippo, J.P.
Faletti, A.G.
Leptin modulates the expression of its receptors in the hypothalamic-pituitary - Ovarian axis in a differential way
topic_facet gonadorelin
leptin
leptin receptor
progesterone
animal experiment
animal tissue
article
female
hormone synthesis
hypothalamus hypophysis ovarian axis
hypothalamus hypophysis system
immunohistochemistry
nonhuman
ovulation
priority journal
process control
protein content
protein expression
radioimmunoassay
rat
tissue level
Western blotting
Animals
Blotting, Western
Chorionic Gonadotropin
Female
Gene Expression
Gonadotropin-Releasing Hormone
Hypothalamus
Immunohistochemistry
Leptin
Ovary
Pituitary Gland
Rats
Rats, Sprague-Dawley
Receptors, Leptin
description To investigate the expression of leptin receptors (Ob-R) in the rat hypothalamus-pituitary-ovarian axis, immature rats were treated with eCG/ hCG and Ob-R expression was evaluated by western blot analysis. The Ob-R expression increased 24 h after eCG administration in all the tissues assayed. In the hypothalamus, these levels immediately decreased to those obtained without treatment. In the pituitary, the Ob-R expression continued to be elevated 48 h after eCG administration, whereas the hCG injection did not modify these levels. Similar results were obtained with the ovarian long isoform. To assess the effect of leptin on its receptors, Ob-R was assessed in hypothalamus, pituitary and ovarian explants cultured in the presence or absence of leptin (0.3-500 ng/ml). In the hypothalamus, we found a biphasic effect: the Ob-R expression was either reduced or increased at low or high concentrations of leptin respectively. LH-releasing hormone secretion increased at 1 ng/ml. In the pituitary, Ob-R increased at 10 or 30 ng/ml of leptin for the long and short isoforms respectively. Leptin also induced an increase in LH release at 30 ng/ml. In the ovarian culture, the presence of leptin produced an increase in Ob-R expression at different ranges of concentrations and a dose-dependent biphasic effect on the progesterone, production. In conclusion, all these results clearly suggest that leptin is able to modulate the expression of its own receptors in the reproductive axis in a differential way. Moreover, the positive or negative effect that leptin exerts on the ovulatory process may be dependent on this regulation. © 2008 Society for Endocrinology.
format Artículo
Artículo
publishedVersion
author Di Yorio, M.P.
Bilbao, M.G.
Pustovrh, M.C.
Prestifilippo, J.P.
Faletti, A.G.
author_facet Di Yorio, M.P.
Bilbao, M.G.
Pustovrh, M.C.
Prestifilippo, J.P.
Faletti, A.G.
author_sort Di Yorio, M.P.
title Leptin modulates the expression of its receptors in the hypothalamic-pituitary - Ovarian axis in a differential way
title_short Leptin modulates the expression of its receptors in the hypothalamic-pituitary - Ovarian axis in a differential way
title_full Leptin modulates the expression of its receptors in the hypothalamic-pituitary - Ovarian axis in a differential way
title_fullStr Leptin modulates the expression of its receptors in the hypothalamic-pituitary - Ovarian axis in a differential way
title_full_unstemmed Leptin modulates the expression of its receptors in the hypothalamic-pituitary - Ovarian axis in a differential way
title_sort leptin modulates the expression of its receptors in the hypothalamic-pituitary - ovarian axis in a differential way
publishDate 2008
url http://hdl.handle.net/20.500.12110/paper_00220795_v198_n2_p355_DiYorio
https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00220795_v198_n2_p355_DiYorio_oai
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AT pustovrhmc leptinmodulatestheexpressionofitsreceptorsinthehypothalamicpituitaryovarianaxisinadifferentialway
AT prestifilippojp leptinmodulatestheexpressionofitsreceptorsinthehypothalamicpituitaryovarianaxisinadifferentialway
AT falettiag leptinmodulatestheexpressionofitsreceptorsinthehypothalamicpituitaryovarianaxisinadifferentialway
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