The yeast endocytic protein Epsin 2 functions in a cell-division signaling pathway
The epsins are a family of adaptors involved in recruiting other endocytic proteins, binding of ubiquitylated cargo and induction of membrane curvature. These molecules bear a characteristic epsin N-terminal homology (ENTH) domain and multiple peptide motifs that mediate protein-protein interactions...
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2009
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00219533_v122_n14_p2453_Mukherjee http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00219533_v122_n14_p2453_Mukherjee_oai |
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I28-R145-paper_00219533_v122_n14_p2453_Mukherjee_oai2020-10-19 Mukherjee, D. Coon, B.G. Edwards III, D.F. Hanna, C.B. Longhi, S.A. McCaffery, J.M. Wendland, B. Retegui, L.A. Bi, E. Aguilar, R.C. 2009 The epsins are a family of adaptors involved in recruiting other endocytic proteins, binding of ubiquitylated cargo and induction of membrane curvature. These molecules bear a characteristic epsin N-terminal homology (ENTH) domain and multiple peptide motifs that mediate protein-protein interactions. We have previously demonstrated that the ENTH domain of epsin is involved in Cdc42 signaling regulation. Here, we present evidence that yeast epsin 2 (Ent2) plays a signaling role during cell division. We observed that overexpression of the ENTH domain of Ent2 (ENTH2), but not Ent1, promoted the formation of chains of cells and aberrant septa. This dominant-negative effect resulted from ENTH2-mediated interference with septin assembly pathways. We mapped the ENTH2 determinants responsible for induction of the phenotype and found them to be important for efficient binding to the septin regulatory protein, Bem3. Supporting a physiological role for epsin 2 in cell division, the protein localized to sites of polarized growth and cytokinesis and rescued a defect in cell division induced by Bem3 misregulation. Collectively, our findings provide a potential molecular mechanism linking endocytosis (via epsin 2) with signaling pathways regulating cell division. application/pdf http://hdl.handle.net/20.500.12110/paper_00219533_v122_n14_p2453_Mukherjee info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar J. Cell Sci. 2009;122(14):2453-2463 Cell division Endocytosis Epsin Septin article error priority journal Adaptor Proteins, Vesicular Transport Amino Acid Sequence Animals Cell Division Cell Polarity Chitin Synthase Cytokinesis Endocytosis Gene Expression Regulation, Fungal Genotype GTPase-Activating Proteins Mice Mice, Inbred BALB C Molecular Sequence Data Phenotype Protein Structure, Tertiary Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Signal Transduction Vesicular Transport Proteins The yeast endocytic protein Epsin 2 functions in a cell-division signaling pathway info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00219533_v122_n14_p2453_Mukherjee_oai |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| topic |
Cell division Endocytosis Epsin Septin article error priority journal Adaptor Proteins, Vesicular Transport Amino Acid Sequence Animals Cell Division Cell Polarity Chitin Synthase Cytokinesis Endocytosis Gene Expression Regulation, Fungal Genotype GTPase-Activating Proteins Mice Mice, Inbred BALB C Molecular Sequence Data Phenotype Protein Structure, Tertiary Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Signal Transduction Vesicular Transport Proteins |
| spellingShingle |
Cell division Endocytosis Epsin Septin article error priority journal Adaptor Proteins, Vesicular Transport Amino Acid Sequence Animals Cell Division Cell Polarity Chitin Synthase Cytokinesis Endocytosis Gene Expression Regulation, Fungal Genotype GTPase-Activating Proteins Mice Mice, Inbred BALB C Molecular Sequence Data Phenotype Protein Structure, Tertiary Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Signal Transduction Vesicular Transport Proteins Mukherjee, D. Coon, B.G. Edwards III, D.F. Hanna, C.B. Longhi, S.A. McCaffery, J.M. Wendland, B. Retegui, L.A. Bi, E. Aguilar, R.C. The yeast endocytic protein Epsin 2 functions in a cell-division signaling pathway |
| topic_facet |
Cell division Endocytosis Epsin Septin article error priority journal Adaptor Proteins, Vesicular Transport Amino Acid Sequence Animals Cell Division Cell Polarity Chitin Synthase Cytokinesis Endocytosis Gene Expression Regulation, Fungal Genotype GTPase-Activating Proteins Mice Mice, Inbred BALB C Molecular Sequence Data Phenotype Protein Structure, Tertiary Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Signal Transduction Vesicular Transport Proteins |
| description |
The epsins are a family of adaptors involved in recruiting other endocytic proteins, binding of ubiquitylated cargo and induction of membrane curvature. These molecules bear a characteristic epsin N-terminal homology (ENTH) domain and multiple peptide motifs that mediate protein-protein interactions. We have previously demonstrated that the ENTH domain of epsin is involved in Cdc42 signaling regulation. Here, we present evidence that yeast epsin 2 (Ent2) plays a signaling role during cell division. We observed that overexpression of the ENTH domain of Ent2 (ENTH2), but not Ent1, promoted the formation of chains of cells and aberrant septa. This dominant-negative effect resulted from ENTH2-mediated interference with septin assembly pathways. We mapped the ENTH2 determinants responsible for induction of the phenotype and found them to be important for efficient binding to the septin regulatory protein, Bem3. Supporting a physiological role for epsin 2 in cell division, the protein localized to sites of polarized growth and cytokinesis and rescued a defect in cell division induced by Bem3 misregulation. Collectively, our findings provide a potential molecular mechanism linking endocytosis (via epsin 2) with signaling pathways regulating cell division. |
| format |
Artículo Artículo publishedVersion |
| author |
Mukherjee, D. Coon, B.G. Edwards III, D.F. Hanna, C.B. Longhi, S.A. McCaffery, J.M. Wendland, B. Retegui, L.A. Bi, E. Aguilar, R.C. |
| author_facet |
Mukherjee, D. Coon, B.G. Edwards III, D.F. Hanna, C.B. Longhi, S.A. McCaffery, J.M. Wendland, B. Retegui, L.A. Bi, E. Aguilar, R.C. |
| author_sort |
Mukherjee, D. |
| title |
The yeast endocytic protein Epsin 2 functions in a cell-division signaling pathway |
| title_short |
The yeast endocytic protein Epsin 2 functions in a cell-division signaling pathway |
| title_full |
The yeast endocytic protein Epsin 2 functions in a cell-division signaling pathway |
| title_fullStr |
The yeast endocytic protein Epsin 2 functions in a cell-division signaling pathway |
| title_full_unstemmed |
The yeast endocytic protein Epsin 2 functions in a cell-division signaling pathway |
| title_sort |
yeast endocytic protein epsin 2 functions in a cell-division signaling pathway |
| publishDate |
2009 |
| url |
http://hdl.handle.net/20.500.12110/paper_00219533_v122_n14_p2453_Mukherjee http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00219533_v122_n14_p2453_Mukherjee_oai |
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