Polyamines and regulation of ornithine biosynthesis in Escherichia coli
The growth rate of several polyamine-deficient mutants of Escherichia coli was very low in minimal medium and increased markedly upon the addition of putrescine, spermidine, arginine, citrulline, or argininosuccinic acid. The endogenous content of polyamines was not significantly altered by the supp...
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1989
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00219193_v171_n4_p1998_Cataldi https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00219193_v171_n4_p1998_Cataldi_oai |
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I28-R145-paper_00219193_v171_n4_p1998_Cataldi_oai2024-08-16 Cataldi, A.A. Algranati, I.D. 1989 The growth rate of several polyamine-deficient mutants of Escherichia coli was very low in minimal medium and increased markedly upon the addition of putrescine, spermidine, arginine, citrulline, or argininosuccinic acid. The endogenous content of polyamines was not significantly altered by the supplementation of polyamine-starved cultures with arginine or its precursors. In contrast, these compounds as well as putrescine or spermidine caused a 40-fold reduction in intracellular ornithine levels when added to polyamine-depleted bacteria. In vivo experiments with radioactive glutamic acid as a precursor and in vitro assay of the related enzymes showed that the decrease in ornithine levels was due to the inhibition of its biosynthesis rather than to an increase in its conversion to citrulline or Δ1-pyrroline-5-carboxylic acid and proline. High endogenous concentrations of ornithine were toxic for the E. coli strains tested. The described results indicate that the stimulatory effect of putrescine and spermidine on the growth of certain polyamine-starved bacteria may be partially due to the control of ornithine biosynthesis by polyamines. Fil:Cataldi, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Algranati, I.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_00219193_v171_n4_p1998_Cataldi info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar J. BACTERIOL. 1989;171(4):1998-2002 ornithine polyamine putrescine spermidine bacterium mutant biosynthesis Escherichia coli growth rate nonhuman priority journal Bacteria (microorganisms) Escherichia coli Polyamines and regulation of ornithine biosynthesis in Escherichia coli info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00219193_v171_n4_p1998_Cataldi_oai |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| topic |
ornithine polyamine putrescine spermidine bacterium mutant biosynthesis Escherichia coli growth rate nonhuman priority journal Bacteria (microorganisms) Escherichia coli |
| spellingShingle |
ornithine polyamine putrescine spermidine bacterium mutant biosynthesis Escherichia coli growth rate nonhuman priority journal Bacteria (microorganisms) Escherichia coli Cataldi, A.A. Algranati, I.D. Polyamines and regulation of ornithine biosynthesis in Escherichia coli |
| topic_facet |
ornithine polyamine putrescine spermidine bacterium mutant biosynthesis Escherichia coli growth rate nonhuman priority journal Bacteria (microorganisms) Escherichia coli |
| description |
The growth rate of several polyamine-deficient mutants of Escherichia coli was very low in minimal medium and increased markedly upon the addition of putrescine, spermidine, arginine, citrulline, or argininosuccinic acid. The endogenous content of polyamines was not significantly altered by the supplementation of polyamine-starved cultures with arginine or its precursors. In contrast, these compounds as well as putrescine or spermidine caused a 40-fold reduction in intracellular ornithine levels when added to polyamine-depleted bacteria. In vivo experiments with radioactive glutamic acid as a precursor and in vitro assay of the related enzymes showed that the decrease in ornithine levels was due to the inhibition of its biosynthesis rather than to an increase in its conversion to citrulline or Δ1-pyrroline-5-carboxylic acid and proline. High endogenous concentrations of ornithine were toxic for the E. coli strains tested. The described results indicate that the stimulatory effect of putrescine and spermidine on the growth of certain polyamine-starved bacteria may be partially due to the control of ornithine biosynthesis by polyamines. |
| format |
Artículo Artículo publishedVersion |
| author |
Cataldi, A.A. Algranati, I.D. |
| author_facet |
Cataldi, A.A. Algranati, I.D. |
| author_sort |
Cataldi, A.A. |
| title |
Polyamines and regulation of ornithine biosynthesis in Escherichia coli |
| title_short |
Polyamines and regulation of ornithine biosynthesis in Escherichia coli |
| title_full |
Polyamines and regulation of ornithine biosynthesis in Escherichia coli |
| title_fullStr |
Polyamines and regulation of ornithine biosynthesis in Escherichia coli |
| title_full_unstemmed |
Polyamines and regulation of ornithine biosynthesis in Escherichia coli |
| title_sort |
polyamines and regulation of ornithine biosynthesis in escherichia coli |
| publishDate |
1989 |
| url |
http://hdl.handle.net/20.500.12110/paper_00219193_v171_n4_p1998_Cataldi https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00219193_v171_n4_p1998_Cataldi_oai |
| work_keys_str_mv |
AT cataldiaa polyaminesandregulationofornithinebiosynthesisinescherichiacoli AT algranatiid polyaminesandregulationofornithinebiosynthesisinescherichiacoli |
| _version_ |
1809357062169165824 |