Quantitative single particle tracking of NGF-receptor complexes: Transport is bidirectional but biased by longer retrograde run lengths
The retrograde transport of nerve growth factor (NGF) in neurite-like processes of living differentiated PC12 cells was studied using streptavidin-quantum dots (QDs) coupled to monobiotin-NGF. These reagents were active in differentiation, binding, internalization, and transport. Ten-35% of the QD-N...
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2007
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00145793_v581_n16_p2905_Echarte https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00145793_v581_n16_p2905_Echarte_oai |
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I28-R145-paper_00145793_v581_n16_p2905_Echarte_oai2024-08-16 Echarte, M.M. Bruno, L. Arndt-Jovin, D.J. Jovin, T.M. Pietrasanta, L.I. 2007 The retrograde transport of nerve growth factor (NGF) in neurite-like processes of living differentiated PC12 cells was studied using streptavidin-quantum dots (QDs) coupled to monobiotin-NGF. These reagents were active in differentiation, binding, internalization, and transport. Ten-35% of the QD-NGF-receptor complexes were mobile. Quantitative single particle tracking revealed a bidirectional step-like motion, requiring intact microtubules, with a net retrograde velocity of 0.054 ± 0.020 μm/s. Individual runs had a mean velocity of ∼0.15 μm/s at room temperature, and the run times were exponentially distributed. The photostability and brightness of QDs permit extended real-time analysis of individual QDbNGF- receptor complexes trafficking within neurites. © 2007 Federation of European Biochemical Societies. Fil:Bruno, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_00145793_v581_n16_p2905_Echarte info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar FEBS Lett. 2007;581(16):2905-2913 Live cell imaging Neurotrophins NGF Quantum dots Retrograde axonal transport nerve growth factor receptor quantum dot streptavidin animal cell article cell differentiation cell labeling controlled study imaging internalization microtubule mouse neurite nonhuman priority journal protein binding protein transport quantitative assay rat room temperature velocity Animals Chromogenic Compounds Endocytosis Microtubules Multiprotein Complexes Neurites PC12 Cells Protein Binding Protein Transport Quantum Dots Rats Receptor, Nerve Growth Factor Sensitivity and Specificity Staining and Labeling Substrate Specificity Quantitative single particle tracking of NGF-receptor complexes: Transport is bidirectional but biased by longer retrograde run lengths info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00145793_v581_n16_p2905_Echarte_oai |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| topic |
Live cell imaging Neurotrophins NGF Quantum dots Retrograde axonal transport nerve growth factor receptor quantum dot streptavidin animal cell article cell differentiation cell labeling controlled study imaging internalization microtubule mouse neurite nonhuman priority journal protein binding protein transport quantitative assay rat room temperature velocity Animals Chromogenic Compounds Endocytosis Microtubules Multiprotein Complexes Neurites PC12 Cells Protein Binding Protein Transport Quantum Dots Rats Receptor, Nerve Growth Factor Sensitivity and Specificity Staining and Labeling Substrate Specificity |
| spellingShingle |
Live cell imaging Neurotrophins NGF Quantum dots Retrograde axonal transport nerve growth factor receptor quantum dot streptavidin animal cell article cell differentiation cell labeling controlled study imaging internalization microtubule mouse neurite nonhuman priority journal protein binding protein transport quantitative assay rat room temperature velocity Animals Chromogenic Compounds Endocytosis Microtubules Multiprotein Complexes Neurites PC12 Cells Protein Binding Protein Transport Quantum Dots Rats Receptor, Nerve Growth Factor Sensitivity and Specificity Staining and Labeling Substrate Specificity Echarte, M.M. Bruno, L. Arndt-Jovin, D.J. Jovin, T.M. Pietrasanta, L.I. Quantitative single particle tracking of NGF-receptor complexes: Transport is bidirectional but biased by longer retrograde run lengths |
| topic_facet |
Live cell imaging Neurotrophins NGF Quantum dots Retrograde axonal transport nerve growth factor receptor quantum dot streptavidin animal cell article cell differentiation cell labeling controlled study imaging internalization microtubule mouse neurite nonhuman priority journal protein binding protein transport quantitative assay rat room temperature velocity Animals Chromogenic Compounds Endocytosis Microtubules Multiprotein Complexes Neurites PC12 Cells Protein Binding Protein Transport Quantum Dots Rats Receptor, Nerve Growth Factor Sensitivity and Specificity Staining and Labeling Substrate Specificity |
| description |
The retrograde transport of nerve growth factor (NGF) in neurite-like processes of living differentiated PC12 cells was studied using streptavidin-quantum dots (QDs) coupled to monobiotin-NGF. These reagents were active in differentiation, binding, internalization, and transport. Ten-35% of the QD-NGF-receptor complexes were mobile. Quantitative single particle tracking revealed a bidirectional step-like motion, requiring intact microtubules, with a net retrograde velocity of 0.054 ± 0.020 μm/s. Individual runs had a mean velocity of ∼0.15 μm/s at room temperature, and the run times were exponentially distributed. The photostability and brightness of QDs permit extended real-time analysis of individual QDbNGF- receptor complexes trafficking within neurites. © 2007 Federation of European Biochemical Societies. |
| format |
Artículo Artículo publishedVersion |
| author |
Echarte, M.M. Bruno, L. Arndt-Jovin, D.J. Jovin, T.M. Pietrasanta, L.I. |
| author_facet |
Echarte, M.M. Bruno, L. Arndt-Jovin, D.J. Jovin, T.M. Pietrasanta, L.I. |
| author_sort |
Echarte, M.M. |
| title |
Quantitative single particle tracking of NGF-receptor complexes: Transport is bidirectional but biased by longer retrograde run lengths |
| title_short |
Quantitative single particle tracking of NGF-receptor complexes: Transport is bidirectional but biased by longer retrograde run lengths |
| title_full |
Quantitative single particle tracking of NGF-receptor complexes: Transport is bidirectional but biased by longer retrograde run lengths |
| title_fullStr |
Quantitative single particle tracking of NGF-receptor complexes: Transport is bidirectional but biased by longer retrograde run lengths |
| title_full_unstemmed |
Quantitative single particle tracking of NGF-receptor complexes: Transport is bidirectional but biased by longer retrograde run lengths |
| title_sort |
quantitative single particle tracking of ngf-receptor complexes: transport is bidirectional but biased by longer retrograde run lengths |
| publishDate |
2007 |
| url |
http://hdl.handle.net/20.500.12110/paper_00145793_v581_n16_p2905_Echarte https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00145793_v581_n16_p2905_Echarte_oai |
| work_keys_str_mv |
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| _version_ |
1809357055919652864 |