Dual effects of organic solvents on chloroplast phosphoribulokinase and NADP-glyceraldehyde-3-P dehydrogenase

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Organic solvents miscible in water (cosolvents) exerted a dual effect on the activation stage of two thioredoxin-linked enzymes of the reductive pentose phosphate cycle, phosphoribulokinase and NADP-glyceraldehyde-3-P dehydrogenase, both from spinach chloroplast; the enzyme specific activity was sti...

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Autores principales: Wolosiuk, R.A., Corley, E., Crawford, N.A., Buchanan, B.B.
Formato: Artículo publishedVersion
Publicado: 1985
Materias:
Chloroplast enzyme
Enzyme activation tPhotosynthesis
NADP-glyceraldehyde-3-phosphate dehydrogenase
Organic solvent
Phosphoribulokinase
organic solvent
phosphoribulokinase
chloroplast
enzyme activation
higher plant
nicotinamide adenine dinucleotide phosphate glyceraldehyde 3 phosphate dehydrogenase
nonhuman
photosynthesis
priority journal
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00145793_v189_n2_p212_Wolosiuk
http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00145793_v189_n2_p212_Wolosiuk_oai
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Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires
id I28-R145-paper_00145793_v189_n2_p212_Wolosiuk_oai
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spelling I28-R145-paper_00145793_v189_n2_p212_Wolosiuk_oai2020-10-19 Wolosiuk, R.A. Corley, E. Crawford, N.A. Buchanan, B.B. 1985 Organic solvents miscible in water (cosolvents) exerted a dual effect on the activation stage of two thioredoxin-linked enzymes of the reductive pentose phosphate cycle, phosphoribulokinase and NADP-glyceraldehyde-3-P dehydrogenase, both from spinach chloroplast; the enzyme specific activity was stimulated and inhibited by low and high concentrations of alcohols, respectively. On the contrary, cosolvents inhibited the catalytic process. In the stimulation of phosphoribulokinase activation, organic solvents reduced the requirement for thioredoxin-f and changed the thiol specificity, so that monothiols became functional. The cosolvent-mediated enhancement of NADP-glyceraldehyde-3-P dehydrogenase was obtained in the absence of modulators. With both enzymes, the concentration of the organic solvents required for activation was inversely proportional to its hydrophobicity (1-butanol < 1-propanol < 2-propanol < ethanol). The present results demonstrate the participation of a new component, the enzyme microenvironment, in the regulation of thioredoxin-linked chloroplast enzymes. © 1985. Fil:Wolosiuk, R.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_00145793_v189_n2_p212_Wolosiuk info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar FEBS Lett. 1985;189(2):212-216 Chloroplast enzyme Enzyme activation tPhotosynthesis NADP-glyceraldehyde-3-phosphate dehydrogenase Organic solvent Phosphoribulokinase organic solvent phosphoribulokinase chloroplast enzyme activation higher plant nicotinamide adenine dinucleotide phosphate glyceraldehyde 3 phosphate dehydrogenase nonhuman photosynthesis priority journal Dual effects of organic solvents on chloroplast phosphoribulokinase and NADP-glyceraldehyde-3-P dehydrogenase info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00145793_v189_n2_p212_Wolosiuk_oai
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-145
collection Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic Chloroplast enzyme
Enzyme activation tPhotosynthesis
NADP-glyceraldehyde-3-phosphate dehydrogenase
Organic solvent
Phosphoribulokinase
organic solvent
phosphoribulokinase
chloroplast
enzyme activation
higher plant
nicotinamide adenine dinucleotide phosphate glyceraldehyde 3 phosphate dehydrogenase
nonhuman
photosynthesis
priority journal
spellingShingle Chloroplast enzyme
Enzyme activation tPhotosynthesis
NADP-glyceraldehyde-3-phosphate dehydrogenase
Organic solvent
Phosphoribulokinase
organic solvent
phosphoribulokinase
chloroplast
enzyme activation
higher plant
nicotinamide adenine dinucleotide phosphate glyceraldehyde 3 phosphate dehydrogenase
nonhuman
photosynthesis
priority journal
Wolosiuk, R.A.
Corley, E.
Crawford, N.A.
Buchanan, B.B.
Dual effects of organic solvents on chloroplast phosphoribulokinase and NADP-glyceraldehyde-3-P dehydrogenase
topic_facet Chloroplast enzyme
Enzyme activation tPhotosynthesis
NADP-glyceraldehyde-3-phosphate dehydrogenase
Organic solvent
Phosphoribulokinase
organic solvent
phosphoribulokinase
chloroplast
enzyme activation
higher plant
nicotinamide adenine dinucleotide phosphate glyceraldehyde 3 phosphate dehydrogenase
nonhuman
photosynthesis
priority journal
description Organic solvents miscible in water (cosolvents) exerted a dual effect on the activation stage of two thioredoxin-linked enzymes of the reductive pentose phosphate cycle, phosphoribulokinase and NADP-glyceraldehyde-3-P dehydrogenase, both from spinach chloroplast; the enzyme specific activity was stimulated and inhibited by low and high concentrations of alcohols, respectively. On the contrary, cosolvents inhibited the catalytic process. In the stimulation of phosphoribulokinase activation, organic solvents reduced the requirement for thioredoxin-f and changed the thiol specificity, so that monothiols became functional. The cosolvent-mediated enhancement of NADP-glyceraldehyde-3-P dehydrogenase was obtained in the absence of modulators. With both enzymes, the concentration of the organic solvents required for activation was inversely proportional to its hydrophobicity (1-butanol < 1-propanol < 2-propanol < ethanol). The present results demonstrate the participation of a new component, the enzyme microenvironment, in the regulation of thioredoxin-linked chloroplast enzymes. © 1985.
format Artículo
Artículo
publishedVersion
author Wolosiuk, R.A.
Corley, E.
Crawford, N.A.
Buchanan, B.B.
author_facet Wolosiuk, R.A.
Corley, E.
Crawford, N.A.
Buchanan, B.B.
author_sort Wolosiuk, R.A.
title Dual effects of organic solvents on chloroplast phosphoribulokinase and NADP-glyceraldehyde-3-P dehydrogenase
title_short Dual effects of organic solvents on chloroplast phosphoribulokinase and NADP-glyceraldehyde-3-P dehydrogenase
title_full Dual effects of organic solvents on chloroplast phosphoribulokinase and NADP-glyceraldehyde-3-P dehydrogenase
title_fullStr Dual effects of organic solvents on chloroplast phosphoribulokinase and NADP-glyceraldehyde-3-P dehydrogenase
title_full_unstemmed Dual effects of organic solvents on chloroplast phosphoribulokinase and NADP-glyceraldehyde-3-P dehydrogenase
title_sort dual effects of organic solvents on chloroplast phosphoribulokinase and nadp-glyceraldehyde-3-p dehydrogenase
publishDate 1985
url http://hdl.handle.net/20.500.12110/paper_00145793_v189_n2_p212_Wolosiuk
http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00145793_v189_n2_p212_Wolosiuk_oai
work_keys_str_mv AT wolosiukra dualeffectsoforganicsolventsonchloroplastphosphoribulokinaseandnadpglyceraldehyde3pdehydrogenase
AT corleye dualeffectsoforganicsolventsonchloroplastphosphoribulokinaseandnadpglyceraldehyde3pdehydrogenase
AT crawfordna dualeffectsoforganicsolventsonchloroplastphosphoribulokinaseandnadpglyceraldehyde3pdehydrogenase
AT buchananbb dualeffectsoforganicsolventsonchloroplastphosphoribulokinaseandnadpglyceraldehyde3pdehydrogenase
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