Nitric oxide inhibits leydig cell steroidogenesis

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Testicular macrophages as well as endothelial cells, which are intimately associated with Leydig cells, constitute a potential source of paracrine nitric oxide (NO) in the testis. In the present study, we investigated the effect of NO donors on MA-10 murine Leydig tumor cell line and rat Leydig cell...

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Autores principales:	Del Punta, K., Charreau, E.H., Pignataro, O.P.
Formato:	Artículo publishedVersion
Publicado:	1996
Materias:	cholesterol cholesterol monooxygenase (side chain cleaving) chorionic gonadotropin cyclic AMP cyclic GMP cytochrome P450 diethylamine diethylenetriamine hemoprotein nitric oxide nitric oxide donor pregnenolone steroid animal cell article autocrine effect controlled study Leydig cell Leydig cell tumor male mouse nonhuman priority journal rat second messenger steroidogenesis testosterone synthesis
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00137227_v137_n12_p5337_DelPunta https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00137227_v137_n12_p5337_DelPunta_oai
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

id	I28-R145-paper_00137227_v137_n12_p5337_DelPunta_oai
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spelling	I28-R145-paper_00137227_v137_n12_p5337_DelPunta_oai2024-08-16 Del Punta, K. Charreau, E.H. Pignataro, O.P. 1996 Testicular macrophages as well as endothelial cells, which are intimately associated with Leydig cells, constitute a potential source of paracrine nitric oxide (NO) in the testis. In the present study, we investigated the effect of NO donors on MA-10 murine Leydig tumor cell line and rat Leydig cell steroidogenesis. We show that NO donors inhibit human CG- induced steroidogenesis in both type of cells. We also studied NO mechanism of action. Contrary to what is observed in many other systems, NO inhibitory effect on Leydig cell steroidogenesis is not mediated by cyclic GMP (cGMP) because NO fails to increase cGMP production, and cGMP analogs do not reproduce NO effect. NO does not modify the production of cAMP, the main second messenger that mediates gonadotropin action. When we studied NO effect over the steroidogenic pathway in MA-10 cells, we found that NO was inhibiting the conversion of cholesterol to pregnenolone. Taken together these results show an inhibitory effect of NO donors on Leydig cell steroidogenesis, and suggest that NO can be directly inhibiting cholesterol side-chain cleavage enzyme (cytochrome P450(scc)) as it does with other heme proteins, including different cytochromes P459. Fil:Del Punta, K. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Charreau, E.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pignataro, O.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_00137227_v137_n12_p5337_DelPunta info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar ENDOCRINOLOGY 1996;137(12):5337-5343 cholesterol cholesterol monooxygenase (side chain cleaving) chorionic gonadotropin cyclic AMP cyclic GMP cytochrome P450 diethylamine diethylenetriamine hemoprotein nitric oxide nitric oxide donor pregnenolone steroid animal cell article autocrine effect controlled study Leydig cell Leydig cell tumor male mouse nonhuman priority journal rat second messenger steroidogenesis testosterone synthesis Nitric oxide inhibits leydig cell steroidogenesis info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00137227_v137_n12_p5337_DelPunta_oai
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic	cholesterol cholesterol monooxygenase (side chain cleaving) chorionic gonadotropin cyclic AMP cyclic GMP cytochrome P450 diethylamine diethylenetriamine hemoprotein nitric oxide nitric oxide donor pregnenolone steroid animal cell article autocrine effect controlled study Leydig cell Leydig cell tumor male mouse nonhuman priority journal rat second messenger steroidogenesis testosterone synthesis
spellingShingle	cholesterol cholesterol monooxygenase (side chain cleaving) chorionic gonadotropin cyclic AMP cyclic GMP cytochrome P450 diethylamine diethylenetriamine hemoprotein nitric oxide nitric oxide donor pregnenolone steroid animal cell article autocrine effect controlled study Leydig cell Leydig cell tumor male mouse nonhuman priority journal rat second messenger steroidogenesis testosterone synthesis Del Punta, K. Charreau, E.H. Pignataro, O.P. Nitric oxide inhibits leydig cell steroidogenesis
topic_facet	cholesterol cholesterol monooxygenase (side chain cleaving) chorionic gonadotropin cyclic AMP cyclic GMP cytochrome P450 diethylamine diethylenetriamine hemoprotein nitric oxide nitric oxide donor pregnenolone steroid animal cell article autocrine effect controlled study Leydig cell Leydig cell tumor male mouse nonhuman priority journal rat second messenger steroidogenesis testosterone synthesis
description	Testicular macrophages as well as endothelial cells, which are intimately associated with Leydig cells, constitute a potential source of paracrine nitric oxide (NO) in the testis. In the present study, we investigated the effect of NO donors on MA-10 murine Leydig tumor cell line and rat Leydig cell steroidogenesis. We show that NO donors inhibit human CG- induced steroidogenesis in both type of cells. We also studied NO mechanism of action. Contrary to what is observed in many other systems, NO inhibitory effect on Leydig cell steroidogenesis is not mediated by cyclic GMP (cGMP) because NO fails to increase cGMP production, and cGMP analogs do not reproduce NO effect. NO does not modify the production of cAMP, the main second messenger that mediates gonadotropin action. When we studied NO effect over the steroidogenic pathway in MA-10 cells, we found that NO was inhibiting the conversion of cholesterol to pregnenolone. Taken together these results show an inhibitory effect of NO donors on Leydig cell steroidogenesis, and suggest that NO can be directly inhibiting cholesterol side-chain cleavage enzyme (cytochrome P450(scc)) as it does with other heme proteins, including different cytochromes P459.
format	Artículo Artículo publishedVersion
author	Del Punta, K. Charreau, E.H. Pignataro, O.P.
author_facet	Del Punta, K. Charreau, E.H. Pignataro, O.P.
author_sort	Del Punta, K.
title	Nitric oxide inhibits leydig cell steroidogenesis
title_short	Nitric oxide inhibits leydig cell steroidogenesis
title_full	Nitric oxide inhibits leydig cell steroidogenesis
title_fullStr	Nitric oxide inhibits leydig cell steroidogenesis
title_full_unstemmed	Nitric oxide inhibits leydig cell steroidogenesis
title_sort	nitric oxide inhibits leydig cell steroidogenesis
publishDate	1996
url	http://hdl.handle.net/20.500.12110/paper_00137227_v137_n12_p5337_DelPunta https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00137227_v137_n12_p5337_DelPunta_oai
work_keys_str_mv	AT delpuntak nitricoxideinhibitsleydigcellsteroidogenesis AT charreaueh nitricoxideinhibitsleydigcellsteroidogenesis AT pignataroop nitricoxideinhibitsleydigcellsteroidogenesis
_version_	1809356956800909312

Nitric oxide inhibits leydig cell steroidogenesis

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