Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line

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δ-aminolevulinic acid (ALA) is the precursor in the biosynthesis of porphyrins. The knowledge of both the regulation of ALA entrance and efflux from the cells and the control of porphyrin biosynthesis is essential to improve ALA-mediated photodynamic therapy. In this work, we studied the regulation...

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Autores principales:	Correa García, S., Casas, A., Perotti, C., Batlle, A., Bermúdez Moretti, M.
Formato:	Artículo publishedVersion
Publicado:	2003
Materias:	δ-aminolevulinic acid ALA efflux ALA uptake Photodynamic therapy 4 aminobutyric acid aminolevulinic acid heme derivative porphobilinogen porphyrin succinylacetone animal cell article breast adenocarcinoma cancer cell culture cell transport controlled study diffusion heme synthesis mouse nonhuman photodynamic therapy priority journal protein function protein protein interaction protein transport temperature dependence Adenocarcinoma Aminolevulinic Acid Animals gamma-Aminobutyric Acid Mammary Neoplasms, Animal Mice Photochemotherapy Photosensitizing Agents Porphyrins Tumor Cells, Cultured
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00070920_v89_n1_p173_CorreaGarcia https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00070920_v89_n1_p173_CorreaGarcia_oai
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

id	I28-R145-paper_00070920_v89_n1_p173_CorreaGarcia_oai
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spelling	I28-R145-paper_00070920_v89_n1_p173_CorreaGarcia_oai2024-08-16 Correa García, S. Casas, A. Perotti, C. Batlle, A. Bermúdez Moretti, M. 2003 δ-aminolevulinic acid (ALA) is the precursor in the biosynthesis of porphyrins. The knowledge of both the regulation of ALA entrance and efflux from the cells and the control of porphyrin biosynthesis is essential to improve ALA-mediated photodynamic therapy. In this work, we studied the regulation of ALA uptake and efflux by endogenously accumulated ALA and/or porphyrins in murine mammary adenocarcinoma cells. Under our set of conditions, the haem synthesis inhibitor succinyl acetone completely prevented porphobilinogen and porphyrin synthesis from ALA, and led to an increase in the intracellular ALA pool. However, neither intracellular ALA nor porphyrin pools regulate ALA uptake or efflux during the first 15 min of the process. Based on temperature dependence data, ALA but not γ-aminobutyric acid (GABA) efflux is mediated by a diffusion mechanism. Moreover, the addition of extracellular GABA not only did not influence the rate of ALA efflux but on the contrary it affected ALA uptake, showing the contribution of a saturable mechanism for the uptake, but not for the efflux of ALA from the cells. © 2003 Cancer Research UK. Fil:Correa García, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Casas, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Perotti, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bermúdez Moretti, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_00070920_v89_n1_p173_CorreaGarcia info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Br. J. Cancer 2003;89(1):173-177 δ-aminolevulinic acid ALA efflux ALA uptake Photodynamic therapy 4 aminobutyric acid aminolevulinic acid heme derivative porphobilinogen porphyrin succinylacetone animal cell article breast adenocarcinoma cancer cell culture cell transport controlled study diffusion heme synthesis mouse nonhuman photodynamic therapy priority journal protein function protein protein interaction protein transport temperature dependence Adenocarcinoma Aminolevulinic Acid Animals gamma-Aminobutyric Acid Mammary Neoplasms, Animal Mice Photochemotherapy Photosensitizing Agents Porphyrins Tumor Cells, Cultured Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00070920_v89_n1_p173_CorreaGarcia_oai
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic	δ-aminolevulinic acid ALA efflux ALA uptake Photodynamic therapy 4 aminobutyric acid aminolevulinic acid heme derivative porphobilinogen porphyrin succinylacetone animal cell article breast adenocarcinoma cancer cell culture cell transport controlled study diffusion heme synthesis mouse nonhuman photodynamic therapy priority journal protein function protein protein interaction protein transport temperature dependence Adenocarcinoma Aminolevulinic Acid Animals gamma-Aminobutyric Acid Mammary Neoplasms, Animal Mice Photochemotherapy Photosensitizing Agents Porphyrins Tumor Cells, Cultured
spellingShingle	δ-aminolevulinic acid ALA efflux ALA uptake Photodynamic therapy 4 aminobutyric acid aminolevulinic acid heme derivative porphobilinogen porphyrin succinylacetone animal cell article breast adenocarcinoma cancer cell culture cell transport controlled study diffusion heme synthesis mouse nonhuman photodynamic therapy priority journal protein function protein protein interaction protein transport temperature dependence Adenocarcinoma Aminolevulinic Acid Animals gamma-Aminobutyric Acid Mammary Neoplasms, Animal Mice Photochemotherapy Photosensitizing Agents Porphyrins Tumor Cells, Cultured Correa García, S. Casas, A. Perotti, C. Batlle, A. Bermúdez Moretti, M. Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line
topic_facet	δ-aminolevulinic acid ALA efflux ALA uptake Photodynamic therapy 4 aminobutyric acid aminolevulinic acid heme derivative porphobilinogen porphyrin succinylacetone animal cell article breast adenocarcinoma cancer cell culture cell transport controlled study diffusion heme synthesis mouse nonhuman photodynamic therapy priority journal protein function protein protein interaction protein transport temperature dependence Adenocarcinoma Aminolevulinic Acid Animals gamma-Aminobutyric Acid Mammary Neoplasms, Animal Mice Photochemotherapy Photosensitizing Agents Porphyrins Tumor Cells, Cultured
description	δ-aminolevulinic acid (ALA) is the precursor in the biosynthesis of porphyrins. The knowledge of both the regulation of ALA entrance and efflux from the cells and the control of porphyrin biosynthesis is essential to improve ALA-mediated photodynamic therapy. In this work, we studied the regulation of ALA uptake and efflux by endogenously accumulated ALA and/or porphyrins in murine mammary adenocarcinoma cells. Under our set of conditions, the haem synthesis inhibitor succinyl acetone completely prevented porphobilinogen and porphyrin synthesis from ALA, and led to an increase in the intracellular ALA pool. However, neither intracellular ALA nor porphyrin pools regulate ALA uptake or efflux during the first 15 min of the process. Based on temperature dependence data, ALA but not γ-aminobutyric acid (GABA) efflux is mediated by a diffusion mechanism. Moreover, the addition of extracellular GABA not only did not influence the rate of ALA efflux but on the contrary it affected ALA uptake, showing the contribution of a saturable mechanism for the uptake, but not for the efflux of ALA from the cells. © 2003 Cancer Research UK.
format	Artículo Artículo publishedVersion
author	Correa García, S. Casas, A. Perotti, C. Batlle, A. Bermúdez Moretti, M.
author_facet	Correa García, S. Casas, A. Perotti, C. Batlle, A. Bermúdez Moretti, M.
author_sort	Correa García, S.
title	Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line
title_short	Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line
title_full	Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line
title_fullStr	Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line
title_full_unstemmed	Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line
title_sort	mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line
publishDate	2003
url	http://hdl.handle.net/20.500.12110/paper_00070920_v89_n1_p173_CorreaGarcia https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00070920_v89_n1_p173_CorreaGarcia_oai
work_keys_str_mv	AT correagarcias mechanisticstudiesondaminolevulinicaciduptakeandeffluxinamammaryadenocarcinomacellline AT casasa mechanisticstudiesondaminolevulinicaciduptakeandeffluxinamammaryadenocarcinomacellline AT perottic mechanisticstudiesondaminolevulinicaciduptakeandeffluxinamammaryadenocarcinomacellline AT batllea mechanisticstudiesondaminolevulinicaciduptakeandeffluxinamammaryadenocarcinomacellline AT bermudezmorettim mechanisticstudiesondaminolevulinicaciduptakeandeffluxinamammaryadenocarcinomacellline
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Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line

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