Caracterización de aislamientos clínicos e industriales de Burkholderia contaminans
Burkholderia contaminans is the species of the Burkholderia cepacia complex prevalent in patients with cystic fibrosis (CF) in Argentina. It is also detected in contaminated products and is associated with hospital infections in non-CF patients.\nWe characterize clinical and industrial isolates of B...
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Facultad de Farmacia y Bioquímica
2019
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Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_5909 http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_5909.dir/5909.PDF |
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I28-R145-HWA_59092022-04-06 Burkholderia contaminans is the species of the Burkholderia cepacia complex prevalent in patients with cystic fibrosis (CF) in Argentina. It is also detected in contaminated products and is associated with hospital infections in non-CF patients.\nWe characterize clinical and industrial isolates of B. contaminans (n = 24) both phenotypically and genotypically. Most of the isolates showed a behavior similar to the type strain, although some characteristics such as the production of hemolysis and the formation of biofilms were strain dependent. Resistance to various antibiotics and virulence was demonstrated in alternative models of infection. In six isolates the reaction detected by the BCESM genomic island was positive and the cable pili system was found in none of them.\nBy means of molecular epidemiology tools, the co-localization of clinical and industrial isolates was observed.\nOn the other hand, the genome of an isolation obtained from a CF patient was sequenced; and a large number of genes associated with virulence and resistance to antibiotics were observed.\nFinally we demonstrate that conjugation methods and electroporation can be used successfully in the genetic manipulation of this species.\nThis knowledge could generate an improvement in the treatment of patients; they allow to point out contaminated products as a potential source of infection; and guide possible future studies with B. contaminans. Fil: López De Volder, María Agustina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Buenos Aires, Argentina Facultad de Farmacia y Bioquímica Degrossi, José Julio López De Volder, María Agustina 2019-04-01 Burkholderia contaminans es la especie del complejo Burkholderia cepacia prevalente en pacientes con fibrosis quística (FQ) de Argentina. Además es detectada en productos contaminados y está asociada a infecciones hospitalarias en pacientes no FQ.\n Caracterizamos fenotípica y genotípicamente aislamientos clínicos e industriales de B. contaminans (n=24). La mayoría de los aislamientos presentaron comportamientos similares a la cepa tipo, aunque algunas características como la producción de hemólisis y la formación de biofilms resultaron cepa dependientes. Se demostró la resistencia a diversos antibióticos y virulencia en modelos alternativos de infección. En seis aislamientos la reacción que detecta la isla genómica BCESM fue positiva y en ninguno se encontró el sistema cable pili.\nMediante herramientas de epidemiología molecular observamos la co-localización de aislamientos clínicos e industriales.\nPor otro lado, secuenciamos el genoma de un aislamiento obtenido de un paciente FQ; y observamos un gran número de genes asociados virulencia y resistencia a antibióticos. \nFinalmente demostramos que los métodos de conjugación y electroporación pueden ser empleados exitosamente en la manipulación genética de esta especie. \nEstos conocimientos podrían generar una mejora en el tratamiento de pacientes; permiten señalar a los productos contaminados como potencial fuente de infección; y orientan posibles futuros estudios con B. contaminans. application/pdf Power, Pablo Quiroga, Ceclia Magariños, María del Carmen Burkholderia contaminans Fibrosis quística Productos contaminados Caracterización. spa Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ Ciencias de la vida Caracterización de aislamientos clínicos e industriales de Burkholderia contaminans info:eu-repo/semantics/doctoralThesis info:ar-repo/semantics/tesis doctoral info:eu-repo/semantics/acceptedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_5909 http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_5909.dir/5909.PDF |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-145 |
collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
language |
Español |
orig_language_str_mv |
spa |
topic |
Burkholderia contaminans Fibrosis quística Productos contaminados Caracterización. Ciencias de la vida |
spellingShingle |
Burkholderia contaminans Fibrosis quística Productos contaminados Caracterización. Ciencias de la vida López De Volder, María Agustina Caracterización de aislamientos clínicos e industriales de Burkholderia contaminans |
topic_facet |
Burkholderia contaminans Fibrosis quística Productos contaminados Caracterización. Ciencias de la vida |
description |
Burkholderia contaminans is the species of the Burkholderia cepacia complex prevalent in patients with cystic fibrosis (CF) in Argentina. It is also detected in contaminated products and is associated with hospital infections in non-CF patients.\nWe characterize clinical and industrial isolates of B. contaminans (n = 24) both phenotypically and genotypically. Most of the isolates showed a behavior similar to the type strain, although some characteristics such as the production of hemolysis and the formation of biofilms were strain dependent. Resistance to various antibiotics and virulence was demonstrated in alternative models of infection. In six isolates the reaction detected by the BCESM genomic island was positive and the cable pili system was found in none of them.\nBy means of molecular epidemiology tools, the co-localization of clinical and industrial isolates was observed.\nOn the other hand, the genome of an isolation obtained from a CF patient was sequenced; and a large number of genes associated with virulence and resistance to antibiotics were observed.\nFinally we demonstrate that conjugation methods and electroporation can be used successfully in the genetic manipulation of this species.\nThis knowledge could generate an improvement in the treatment of patients; they allow to point out contaminated products as a potential source of infection; and guide possible future studies with B. contaminans. |
author2 |
Degrossi, José Julio |
author_facet |
Degrossi, José Julio López De Volder, María Agustina |
format |
Tesis doctoral Tesis doctoral acceptedVersion |
author |
López De Volder, María Agustina |
author_sort |
López De Volder, María Agustina |
title |
Caracterización de aislamientos clínicos e industriales de Burkholderia contaminans |
title_short |
Caracterización de aislamientos clínicos e industriales de Burkholderia contaminans |
title_full |
Caracterización de aislamientos clínicos e industriales de Burkholderia contaminans |
title_fullStr |
Caracterización de aislamientos clínicos e industriales de Burkholderia contaminans |
title_full_unstemmed |
Caracterización de aislamientos clínicos e industriales de Burkholderia contaminans |
title_sort |
caracterización de aislamientos clínicos e industriales de burkholderia contaminans |
publisher |
Facultad de Farmacia y Bioquímica |
publishDate |
2019 |
url |
http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_5909 http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_5909.dir/5909.PDF |
work_keys_str_mv |
AT lopezdevoldermariaagustina caracterizaciondeaislamientosclinicoseindustrialesdeburkholderiacontaminans |
_version_ |
1766017552123691008 |