Relación entre la ecotextura del parénquima testicular y la producción y calidad seminal en toros de razas carniceras dadores de semen
Fertility is a key variable in bovine production. Although both sexes take part in the\npregnancy results, the quantitative relationship male:female means that the fertility of bulls plays a fundamental role. In the case of semen-donor bulls located in Semen\nCollection and Processing Centers (SCPC)...
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Facultad de Ciencias Veterinarias
2020
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=avemaster&cl=CL1&d=HWA_5640 http://repositoriouba.sisbi.uba.ar/gsdl/collect/avemaster/index/assoc/HWA_5640.dir/5640.PDF |
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I28-R145-HWA_5640 |
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dspace |
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Universidad de Buenos Aires |
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I-28 |
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R-145 |
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Repositorio Digital de la Universidad de Buenos Aires (UBA) |
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Español |
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spa |
| topic |
Ecotextura Calidad seminal Toros razas carniceras Fertilidad Toro Bovinos para carne Semen Calidad seminal Parénquima testicular Ecotextura Medicina veterinaria |
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Ecotextura Calidad seminal Toros razas carniceras Fertilidad Toro Bovinos para carne Semen Calidad seminal Parénquima testicular Ecotextura Medicina veterinaria Marcantonio, Sergio Ariel Relación entre la ecotextura del parénquima testicular y la producción y calidad seminal en toros de razas carniceras dadores de semen |
| topic_facet |
Ecotextura Calidad seminal Toros razas carniceras Fertilidad Toro Bovinos para carne Semen Calidad seminal Parénquima testicular Ecotextura Medicina veterinaria |
| description |
Fertility is a key variable in bovine production. Although both sexes take part in the\npregnancy results, the quantitative relationship male:female means that the fertility of bulls plays a fundamental role. In the case of semen-donor bulls located in Semen\nCollection and Processing Centers (SCPC), the enormous capacity of artificial insemination for multiplying the reproductive potential makes the importance of male\nfertility even greater. A complete breeding soundness examination is a powerful tool for\nestimating the potential fertility of bulls. In the last twenty years, ultrasonography (US)\nhas begun to be considered a non-invasive, non-traumatic tool for evaluating the structure and function of the male reproductive tract. Computerized analysis of the ultrasound\nimage permits revelation of differences in echotexture that are imperceptible to the human eye and makes objective measurements possible. The objective of this study was to\nevaluate the potential of testicular US to predict the quali/quantitative capacity to produce semen in bulls belonging to SCPC. The work was carried out in the facilities of the CRB,\na SCPC located close to the town of San Antonio de Areco, in the province of Buenos Aires. A total of 57 semen-donor bulls, on a regular semen collection regime, were\nincluded in the study. Bulls were between 1.5 and 10 years of age and comprised the following beef-producing breeds: Angus, Brangus, Braford, Limangus and Polled Hereford. Semen collection was carried out using an artificial vagina. The number of\nejaculates processed and the production of frozen straws during the two weeks before and\nthe two weeks after the time ?0? (day of ultrasound), ?30? (30 days post ultrasound) and ?60? (60 days post ultrasound), were registered. Ultrasound images were taken using an\nEXAGO®\n ultrasound (ECM Angulemme, France) with a multifrequency linear\ntransducer configured at 7.5 MHz. Three transversal images were taken per testicle, in 3 positions: dorsal, medium and ventral. The images were analyzed using the ECOTEXT®\nsoftware. A correlation analysis was carried out between all the Ecotext variables (EC1=\nblack pixels, EC2= white pixels, EC3= mean gray level of pixels, Density= density of hypoechogenic areas, Diameter= mean diameter of hypoechogenic areas and Area\npercentage of hypoechogenic areas in the total area of the region of interets) and semen\nquality and production (time 0, time 30 and time 60). Then a bivariant and multivariant analysis (multinomial logistic regression) was carried out to predict the freezability of the ejaculates as related to breed, age or tubule density (density of hypoechogenic areas) measured by ultrasound. The distribution of the testicular echotexture and microstructure\nobtained in the semen-donor population of bulls were the following (mean ± S.D.):\nEC1:14.96 ± 10.76; EC2: 50.24 ± 105.58; EC3: 86.11 ± 11.83; Area: 11.86 ± 3.40;\nDiameter: 126.08 ± 23.73µm and Density: 137.54 ± 17.67. No significant differences\n(p>0.05) were observed between breeds or between ages for any of the ultrasound parameters considered. When analyzing the relationship between seminal quality and the\nparameters of testicular echotexture and microstructure at time 0, a positive relationship\nwas observed between the density of AH and seminal volume (r=0.32). In regard to sperm morphology, a highly significant negative relationship was observed between the\npercentage of abnormal heads and EC2, EC3 and tubule diameter (r= -0.37; -0.52; -0.37 respectively). Diameter was also negatively correlated with the percentage of other sperm morphological abnormalities (r= -0.30). The quantity of black pixels (EC1) was positively\ncorrelated with the area, density and diameter of the seminiferous tubules (r=0.69; 0.46\nand 0.53, respectively). No correlation was found between the Ecotext variables and\nseminal quality parameters at 30 days after the ultrasound measurements. A positive\ncorrelation was observed between area and diameter and sperm concentration in the\nejaculate 60 days after the ultrasound measurements (0.33 and 0.35, respectively). At day\n0, no correlation was observed between the Ecotext parameters and post-thaw motility.\nSignificant differences in area and diameter were observed between bulls that produced\ngood quality post-thaw ejaculates and those that produced ejaculates that were rejected at\n30 and 60 days post ultrasound. No differences were observed in the Ecotext parameters\nbetween bulls that produced 100 straws per ejaculate, 101 to 150 straws per ejaculate and\nmore than 150 straws per ejaculate. The AH density was the only Ecotext parameter that\npermitted a prediction of the freezing results on day 0. All bulls with up to 115 AH/cm2\nproduced semen that was not apt for freezing. In conclusion, despite having shown\ndifferent levels of association between the Ecotext parameters and semen quality, it\nremains necessary to continue the studies to be able to confirm the use of this tool to predict potential fertility of semen-donor bulls from CCPS. |
| author2 |
Miragaya, Marcelo |
| author_facet |
Miragaya, Marcelo Marcantonio, Sergio Ariel |
| format |
Tesis de maestría Tesis de maestría acceptedVersion |
| author |
Marcantonio, Sergio Ariel |
| author_sort |
Marcantonio, Sergio Ariel |
| title |
Relación entre la ecotextura del parénquima testicular y la producción y calidad seminal en toros de razas carniceras dadores de semen |
| title_short |
Relación entre la ecotextura del parénquima testicular y la producción y calidad seminal en toros de razas carniceras dadores de semen |
| title_full |
Relación entre la ecotextura del parénquima testicular y la producción y calidad seminal en toros de razas carniceras dadores de semen |
| title_fullStr |
Relación entre la ecotextura del parénquima testicular y la producción y calidad seminal en toros de razas carniceras dadores de semen |
| title_full_unstemmed |
Relación entre la ecotextura del parénquima testicular y la producción y calidad seminal en toros de razas carniceras dadores de semen |
| title_sort |
relación entre la ecotextura del parénquima testicular y la producción y calidad seminal en toros de razas carniceras dadores de semen |
| publisher |
Facultad de Ciencias Veterinarias |
| publishDate |
2020 |
| url |
http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=avemaster&cl=CL1&d=HWA_5640 http://repositoriouba.sisbi.uba.ar/gsdl/collect/avemaster/index/assoc/HWA_5640.dir/5640.PDF |
| work_keys_str_mv |
AT marcantoniosergioariel relacionentrelaecotexturadelparenquimatesticularylaproduccionycalidadseminalentorosderazascarnicerasdadoresdesemen |
| _version_ |
1766017546118496256 |
| spelling |
I28-R145-HWA_56402021-11-23 Fertility is a key variable in bovine production. Although both sexes take part in the\npregnancy results, the quantitative relationship male:female means that the fertility of bulls plays a fundamental role. In the case of semen-donor bulls located in Semen\nCollection and Processing Centers (SCPC), the enormous capacity of artificial insemination for multiplying the reproductive potential makes the importance of male\nfertility even greater. A complete breeding soundness examination is a powerful tool for\nestimating the potential fertility of bulls. In the last twenty years, ultrasonography (US)\nhas begun to be considered a non-invasive, non-traumatic tool for evaluating the structure and function of the male reproductive tract. Computerized analysis of the ultrasound\nimage permits revelation of differences in echotexture that are imperceptible to the human eye and makes objective measurements possible. The objective of this study was to\nevaluate the potential of testicular US to predict the quali/quantitative capacity to produce semen in bulls belonging to SCPC. The work was carried out in the facilities of the CRB,\na SCPC located close to the town of San Antonio de Areco, in the province of Buenos Aires. A total of 57 semen-donor bulls, on a regular semen collection regime, were\nincluded in the study. Bulls were between 1.5 and 10 years of age and comprised the following beef-producing breeds: Angus, Brangus, Braford, Limangus and Polled Hereford. Semen collection was carried out using an artificial vagina. The number of\nejaculates processed and the production of frozen straws during the two weeks before and\nthe two weeks after the time ?0? (day of ultrasound), ?30? (30 days post ultrasound) and ?60? (60 days post ultrasound), were registered. Ultrasound images were taken using an\nEXAGO®\n ultrasound (ECM Angulemme, France) with a multifrequency linear\ntransducer configured at 7.5 MHz. Three transversal images were taken per testicle, in 3 positions: dorsal, medium and ventral. The images were analyzed using the ECOTEXT®\nsoftware. A correlation analysis was carried out between all the Ecotext variables (EC1=\nblack pixels, EC2= white pixels, EC3= mean gray level of pixels, Density= density of hypoechogenic areas, Diameter= mean diameter of hypoechogenic areas and Area\npercentage of hypoechogenic areas in the total area of the region of interets) and semen\nquality and production (time 0, time 30 and time 60). Then a bivariant and multivariant analysis (multinomial logistic regression) was carried out to predict the freezability of the ejaculates as related to breed, age or tubule density (density of hypoechogenic areas) measured by ultrasound. The distribution of the testicular echotexture and microstructure\nobtained in the semen-donor population of bulls were the following (mean ± S.D.):\nEC1:14.96 ± 10.76; EC2: 50.24 ± 105.58; EC3: 86.11 ± 11.83; Area: 11.86 ± 3.40;\nDiameter: 126.08 ± 23.73µm and Density: 137.54 ± 17.67. No significant differences\n(p>0.05) were observed between breeds or between ages for any of the ultrasound parameters considered. When analyzing the relationship between seminal quality and the\nparameters of testicular echotexture and microstructure at time 0, a positive relationship\nwas observed between the density of AH and seminal volume (r=0.32). In regard to sperm morphology, a highly significant negative relationship was observed between the\npercentage of abnormal heads and EC2, EC3 and tubule diameter (r= -0.37; -0.52; -0.37 respectively). Diameter was also negatively correlated with the percentage of other sperm morphological abnormalities (r= -0.30). The quantity of black pixels (EC1) was positively\ncorrelated with the area, density and diameter of the seminiferous tubules (r=0.69; 0.46\nand 0.53, respectively). No correlation was found between the Ecotext variables and\nseminal quality parameters at 30 days after the ultrasound measurements. A positive\ncorrelation was observed between area and diameter and sperm concentration in the\nejaculate 60 days after the ultrasound measurements (0.33 and 0.35, respectively). At day\n0, no correlation was observed between the Ecotext parameters and post-thaw motility.\nSignificant differences in area and diameter were observed between bulls that produced\ngood quality post-thaw ejaculates and those that produced ejaculates that were rejected at\n30 and 60 days post ultrasound. No differences were observed in the Ecotext parameters\nbetween bulls that produced 100 straws per ejaculate, 101 to 150 straws per ejaculate and\nmore than 150 straws per ejaculate. The AH density was the only Ecotext parameter that\npermitted a prediction of the freezing results on day 0. All bulls with up to 115 AH/cm2\nproduced semen that was not apt for freezing. In conclusion, despite having shown\ndifferent levels of association between the Ecotext parameters and semen quality, it\nremains necessary to continue the studies to be able to confirm the use of this tool to predict potential fertility of semen-donor bulls from CCPS. Fil: Marcantonio, Sergio Ariel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Buenos Aires. Argentina Miragaya, Marcelo Facultad de Ciencias Veterinarias Gnemmi, Giovanni Marcantonio, Sergio Ariel 2020-08-07 La fertilidad es una de las variables clave en la producción bovina. Si bien ambos sexos\nintervienen en el resultado de preñez, la relación cuantitativa macho:hembra hace que la fertilidad de los toros cobre un papel fundamental. En el caso de los toros dadores de\nsemen alojados en Centros de Colecta y Procesamiento de Semen (CCPS), la enorme capacidad de multiplicar el potencial reproductivo que brinda la inseminación artificial\nlleva a que la importancia de la fertilidad del macho sea aún mayor. El examen\nandrológico completo constituye una poderosa herramienta para estimar la fertilidad potencial de los toros. Desde hace veinte años se ha comenzado a considerar a la\nultrasonografía (US) como una técnica no invasiva y atraumática para evaluar la\nestructura y funcionalidad del aparato reproductor del macho. El análisis computarizado de la imagen ultrasonográfica permite revelar diferencias en la ecotextura imperceptibles\nal ojo humano, posibilitando mediciones objetivas. El objetivo del trabajo fue evaluar el potencial de la US testicular para predecir la capacidad cuali/cuantitiva de producción de semen en los toros de CCPS. El trabajo fue llevado a cabo en las instalaciones del CRB,\nubicado en cercanías de la localidad de San Antonio de Areco, provincia de Buenos Aires. Fueron incorporados al estudio 57 toros dadores en régimen de extracción, de entre 1,5 y 10 años de edad, de las razas carniceras Angus, Brangus, Braford, Limangus y Polled\nHereford. La extracción de semen fue realizada por el método de la vagina artificial. Se registró la cantidad de eyaculados procesados y la producción de pajuelas durante las dos\nsemanas anteriores y dos semanas posteriores a los tiempos ?0? (día de la\nultrasonografía), ?30? (30 días después) y ?60? (60 días después). Las imágenes ultrasonográficas fueron tomadas con un ecógrafo (modelo EXAGO®, ECM Angulemme, Francia) con un transductor lineal multifrecuencia, configurado en 7,5 MHz. Se tomaron 3 imágenes por testículo, en forma transversal en 3 posiciones: dorsal, media\ny ventral. Las imágenes fueron analizadas con el software ECOTEXT®. Se realizó un análisis de correlación entre todas las variables de Ecotext (EC1= cantidad de pixeles\nnegros, EC2= cantidad de pixeles blancos, EC3=nivel medio de pixeles grises, %AH= % de áreas hipoecogénicas, Diámetro= diámetro tubular y Densidad= densidad tubular) y la calidad y producción seminal (tiempo 0, tiempo 30 y tiempo 60). Luego se realizó un II análisis bivariante y multivariante (regresión logística multinomial) para predecir la\ncongelabilidad de los eyaculados en función de la raza, la edad o la densidad de túbulos (densidad de áreas hipoecogénicas) a la ecografía. La distribución de datos de ecotextura\ny microestructura testicular (media ± D.E.) obtenidos en la población de toros dadores fue la siguiente: EC1:14,96±10,76; EC2: 50,24±105,58; EC3: 86,11±11,83; Área:\n11,86±3,40; Diámetro: 126,08 ± 23,73µm y Densidad: 137,54 ± 17,67. No hubo diferencias significativas entre razas ni edades para los parámetros ultrasonográficos\nconsiderados (p>0,05). Al analizar la relación entre los parámetros de ecotextura y\nmicroestructura testicular con la calidad seminal al tiempo 0, pudo observarse una relación positiva entre la densidad de AH y el volumen seminal (r=0,32). En cuanto a la\nmorfología espermática, se pudo comprobar una relación negativa y altamente significativa entre EC2, EC3 y Diámetro tubular y el porcentaje de cabezas anormales (r=\n-0,37; -0,52; -0,37). El Diámetro se relacionó negativamente con el porcentaje de otras\nanomalías (r= -0,30). No se encontró correlación entre las variables de Ecotext y los parámetros de calidad seminal a los 30 días posteriores a la ecografía. A los 60 días\nposteriores al análisis ecográfico se observó una correlación positiva entre el Área y el Diámetro y la concentración espermática del eyaculado (0,33 y 035, respectivamente).\nNo se encontraron correlaciones entre los parámetros de Ecotext y la movilidad posdescongelación al tiempo 0. Existieron diferencias significativas de Área y Diámetro\nentre toros productores de eyaculados de buena calidad a la descongelación y de eyaculados rechazados al tiempo 30 y 60 posecografía. No se observaron diferencias entre\nlos parámetros de Ecotext de los toros que produjeron hasta 100 pajuelas por eyaculado, 101 a 150 pajuelas por eyaculado y más de 150 pajuelas por eyaculado. El valor de\ndensidad de AH fue el único parámetro de Ecotext que permitió predecir el resultado de\nla congelación a tiempo 0. Todos los toros con valores ?115 AH/cm2\n tuvieron semen no\ncongelable. En conclusión, no obstante haberse demostrado distintos niveles de asociación entre los parámetros de Ecotext y la calidad seminal, es necesario continuar\ncon los estudios a los efectos de comprobar la utilidad de esta herramienta en la predicción\nde la fertilidad potencial de los toros dadores de semen de CCPS. \n application/pdf Ecotextura Calidad seminal Toros razas carniceras Fertilidad spa Universidad de Buenos Aires. Facultad de Ciencias Veterinarias info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-ncnd/2.5/ar/ Toro Bovinos para carne Semen Calidad seminal Parénquima testicular Ecotextura Medicina veterinaria Relación entre la ecotextura del parénquima testicular y la producción y calidad seminal en toros de razas carniceras dadores de semen info:eu-repo/semantics/masterThesis info:ar-repo/semantics/tesis de maestría info:eu-repo/semantics/acceptedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=avemaster&cl=CL1&d=HWA_5640 http://repositoriouba.sisbi.uba.ar/gsdl/collect/avemaster/index/assoc/HWA_5640.dir/5640.PDF |