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Ticks are the main vectors involved in pathogen transmission to domestic and wild animals worldwide. In Argentina, the tick species Rhipicephalus microplus is the vector for\ntwo important livestock diseases, the Babesiosis and the Anaplasmosis. The pathogens responsible for these diseases are the p...

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Detalles Bibliográficos
Autor principal:	Guillemi, Eliana Carolina
Otros Autores:	Ruybal, Paula
Formato:	Tesis doctoral acceptedVersion
Lenguaje:	Español
Publicado:	Universidad de Buenos Aires. Facultad de Ciencias Veterinarias 2013
Materias:	Babesia bovis Babesia bigemina Anaplasma marginale MLST Epidemiología molecular Molecular epidemiology Genotipo Hemoparásitos Bovinos Epidemiología
Acceso en línea:	http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=avaposgra&cl=CL1&d=HWA_3636 https://repositoriouba.sisbi.uba.ar/gsdl/collect/avaposgra/index/assoc/HWA_3636.dir/3636.PDF
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

id	I28-R145-HWA_3636
record_format	dspace
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
language	Español
orig_language_str_mv	spa
topic	Babesia bovis Babesia bigemina Anaplasma marginale MLST Epidemiología molecular Babesia bovis Babesia bigemina Anaplasma marginale MLST Molecular epidemiology Genotipo Hemoparásitos Bovinos Babesia bovis Babesia bigemina Anaplasma marginale Epidemiología
spellingShingle	Babesia bovis Babesia bigemina Anaplasma marginale MLST Epidemiología molecular Babesia bovis Babesia bigemina Anaplasma marginale MLST Molecular epidemiology Genotipo Hemoparásitos Bovinos Babesia bovis Babesia bigemina Anaplasma marginale Epidemiología Guillemi, Eliana Carolina 3636
topic_facet	Babesia bovis Babesia bigemina Anaplasma marginale MLST Epidemiología molecular Babesia bovis Babesia bigemina Anaplasma marginale MLST Molecular epidemiology Genotipo Hemoparásitos Bovinos Babesia bovis Babesia bigemina Anaplasma marginale Epidemiología
description	Ticks are the main vectors involved in pathogen transmission to domestic and wild animals worldwide. In Argentina, the tick species Rhipicephalus microplus is the vector for\ntwo important livestock diseases, the Babesiosis and the Anaplasmosis. The pathogens responsible for these diseases are the protozoan Babesia bovis and Babesia bigemina and the bacteria Anaplasma marginale; all of them are obligate intracellular agents of the bovine erythrocyte. The study of population diversity of these pathogens at a genetic level\nrepresents a fundamental approach aimed to elucidate the impact of factors such as transmissibility and the underlying population structure. In this work, we have developed a tool for the epidemiological study at the molecular level based on sequencing of multiple loci (MLST) for each of the three pathogens. The\nimplementation of this tool allowed genotypic characterization and discrimination of\ndifferent isolates. The implementation of phylogenetic studies and statistical programs\nallowed studying aspects related to previous evolutionary processes.\nAs a result of the analysis of field isolates, we obtained findings suggestive of a wide diverse population and presence of co-infections with different genotypic variants in a\nsingle bovine host. We experimentally reproduced co-infection with two distinct genotypes of B. bovis and A. marginale in order to understand the dynamics of the interaction between\nthe two strains in the same animal as a highly likely event in enzootic areas and where high levels of ticks are found. This thesis contributes to the study of the diversity and population structure of hemoparasites B. bovis, B. bigemina and A. marginale through the development of a\nmolecular typing tool.\n
author2	Ruybal, Paula
author_facet	Ruybal, Paula Guillemi, Eliana Carolina
format	Tesis doctoral Tesis doctoral acceptedVersion
author	Guillemi, Eliana Carolina
author_sort	Guillemi, Eliana Carolina
title	3636
title_short	3636
title_full	3636
title_fullStr	3636
title_full_unstemmed	3636
title_sort	3636
publisher	Universidad de Buenos Aires. Facultad de Ciencias Veterinarias
publishDate	2013
url	http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=avaposgra&cl=CL1&d=HWA_3636 https://repositoriouba.sisbi.uba.ar/gsdl/collect/avaposgra/index/assoc/HWA_3636.dir/3636.PDF
work_keys_str_mv	AT guillemielianacarolina 3636 AT guillemielianacarolina caracterizaciondeladiversidadgenotipicadeloshemoparasitosbovinosbabesiabovisbabesiabigeminayanaplasmamarginale
_version_	1824356449188315136
spelling	I28-R145-HWA_36362024-08-27 3636 Ticks are the main vectors involved in pathogen transmission to domestic and wild animals worldwide. In Argentina, the tick species Rhipicephalus microplus is the vector for\ntwo important livestock diseases, the Babesiosis and the Anaplasmosis. The pathogens responsible for these diseases are the protozoan Babesia bovis and Babesia bigemina and the bacteria Anaplasma marginale; all of them are obligate intracellular agents of the bovine erythrocyte. The study of population diversity of these pathogens at a genetic level\nrepresents a fundamental approach aimed to elucidate the impact of factors such as transmissibility and the underlying population structure. In this work, we have developed a tool for the epidemiological study at the molecular level based on sequencing of multiple loci (MLST) for each of the three pathogens. The\nimplementation of this tool allowed genotypic characterization and discrimination of\ndifferent isolates. The implementation of phylogenetic studies and statistical programs\nallowed studying aspects related to previous evolutionary processes.\nAs a result of the analysis of field isolates, we obtained findings suggestive of a wide diverse population and presence of co-infections with different genotypic variants in a\nsingle bovine host. We experimentally reproduced co-infection with two distinct genotypes of B. bovis and A. marginale in order to understand the dynamics of the interaction between\nthe two strains in the same animal as a highly likely event in enzootic areas and where high levels of ticks are found. This thesis contributes to the study of the diversity and population structure of hemoparasites B. bovis, B. bigemina and A. marginale through the development of a\nmolecular typing tool.\n Fil: Guillemi, Eliana Carolina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Buenos Aires, Argentina Ruybal, Paula Wilkowsky, Silvina E. Guillemi, Eliana Carolina 2013-05-03 Las garrapatas son los principales vectores implicados en la transmisión de patógenos a los animales domésticos y de vida silvestre a nivel mundial. En Argentina, la especie Rhipicephalus microplus actúa como vector transmisor de dos enfermedades de relevancia en la actividad ganadera, la Babesiosis y la Anaplasmosis. Los patógenos responsables de\nestas enfermedades son los protozoarios Babesia bovis, Babesia bigemina y la bacteria Anaplasma marginale, siendo los tres agentes intracelulares obligados del eritrocito bovino. El estudio de la diversidad poblacional de estos patógenos a nivel genético constituye un\nenfoque fundamental tendiente a dilucidar el impacto de factores tales como transmisibilidad y estructura poblacional subyacente. En este trabajo, hemos desarrollado una herramienta destinada el estudio epidemiológico a nivel molecular basada en la secuenciación de múltiples loci (MLST) para\ncada uno de los tres patógenos. La aplicación de esta herramienta permitió la\ncaracterización genotípica y la discriminación de distintos aislamientos. La implementación de estudios filogenéticos y programas estadísticos permitió estudiar aspectos vinculados a procesos evolutivos previos.\nProducto del análisis de aislamientos de campo se obtuvieron resultados sugerentes de\nuna gran diversidad poblacional así como presencia de co-infecciones con variantes genotípicas distintas en un mismo hospedador bovino. En tal sentido se reprodujo\nexperimentalmente la coinfección con dos genotipos distintos de B. bovis y de A. marginale a\nfin de comprender la dinámica de la interacción entre dos cepas en un mismo animal como\nun evento altamente probable en zonas enzoóticas y de alta carga de garrapatas.\nEste trabajo de tesis contribuye al estudio de la diversidad y estructura poblacional de\nlos hemoparásitos B. bovis, B. bigemina y A. marginale a través del desarrollo de una herramienta de tipificación molecular de última generación.\n application/pdf Babesia bovis Babesia bigemina Anaplasma marginale MLST Epidemiología molecular Babesia bovis Babesia bigemina Anaplasma marginale MLST Molecular epidemiology spa Universidad de Buenos Aires. Facultad de Ciencias Veterinarias info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-ncnd/2.5/ar/ Genotipo Hemoparásitos Bovinos Babesia bovis Babesia bigemina Anaplasma marginale Epidemiología Epidemiología molecular Doctora de la Universidad de Buenos Aires en Ciencias Veterinarias Caracterización de la diversidad genotípica de los hemoparásitos bovinos Babesia bovis, Babesia bigemina y Anaplasma marginale info:eu-repo/semantics/doctoralThesis info:ar-repo/semantics/tesis doctoral info:eu-repo/semantics/acceptedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=avaposgra&cl=CL1&d=HWA_3636 https://repositoriouba.sisbi.uba.ar/gsdl/collect/avaposgra/index/assoc/HWA_3636.dir/3636.PDF

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