Rol de las Heat Shock Proteins en tejido renal de ratas Zucker Diabetic Fatty

The aim of this study was to evaluate whether the "Heat Shock Proteins" (HSP) can control functional and morphological renal damage in a recognized animal model of type 2 diabetes (DMT2). Male 12-week old "Zucker Diabetic Fatty" (ZDF) rats and control "Lean Zucker Rat¨ (LZR)...

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Autor principal:	Maselli, María del Carmen
Otros Autores:	Angerosa, Margarita
Formato:	Tesis doctoral acceptedVersion
Lenguaje:	Español
Publicado:	Facultad de Farmacia y Bioquímica 2018
Materias:	Nefropatía diabética Ratas Diabetes Diabetes millitus tipo 2 Zucker Diabetic Fatty Heat Shock Proteins Rat Ciencias de la vida
Acceso en línea:	http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_2825 http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_2825.dir/2825.PDF
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

id	I28-R145-HWA_2825
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spelling	I28-R145-HWA_28252020-08-07 The aim of this study was to evaluate whether the "Heat Shock Proteins" (HSP) can control functional and morphological renal damage in a recognized animal model of type 2 diabetes (DMT2). Male 12-week old "Zucker Diabetic Fatty" (ZDF) rats and control "Lean Zucker Rat¨ (LZR) were used. All animals received water and standard rat chow, "ad libitum". The immunoexpression of HSP70 and HSP27 was assessed in kidney tissue. Markers of glomerular and tubulointerstitial damage (oxidative stress, inflammation, nitrosative stress, fat deposition, apoptosis, interstitial fibrosis), and podocyte injury were analyzed. Ultrastructural glomerular alterations were evaluated by electron microscopy. We correlated biochemical variables and histological markers of podocyte damage and expression of HSPs in renal tissue. The conclusion of this study is that the increased expression of HSP70 and HSP27 found in kidney of ZDF rats, although it represents an activated repair mechanism in response to an adverse metabolic scenario, its participation does not succeed in modulating the injury caused in such tissue, showing insufficient or at least incomplete behavior due to the magnitude of renal cell damage induced in this experimental model of T2DM. Fil: Maselli, María del Carmen. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Buenos Aires, Argentina Angerosa, Margarita Facultad de Farmacia y Bioquímica Toblli, Jorge E. Maselli, María del Carmen 2018-12-12 El objetivo de este trabajo fue determinar si las ¨Heat Shock Proteins¨ (HSP), controlan el daño renal funcional y morfológico en un reconocido modelo animal de diabetes tipo 2 (DMT2). Se utilizaron ratas ¨Zucker Diabetic Fatty¨ (ZDF) macho de 12-semanas y ratas control ?Zucker Lean? (LZR). Los animales recibieron agua y alimento estándar para rata, (ad libitum). Se evaluó en el tejido renal la inmunoexpresión de HSP70 y HSP27. Se analizaron marcadores de daño glomerular y túbulointersticial (estrés oxidativo, inflamación, estrés nitrosativo, depósito de grasa, apoptosis, fibrosis intersticial), daño podocítico, y las alteraciones ultraestructurales glomerulares mediante microscopia electrónica. Se correlacionó variables bioquímicas y marcadores histológicos de daño podocítico y expresión de HSPs en tejido renal.\nDe los resultados obtenidos surge la conclusión que la expresión incrementada de HSP70 y HSP27 en riñón de ratas ZDF, si bien representa un mecanismo de reparación activado localmente en respuesta a un escenario metabólico adverso, su participación no logro modular la injuria ocasionada en el tejido renal de estos animales, mostrando un comportamiento insuficiente o al menos incompleto ante la magnitud del daño celular renal generado en este modelo experimental de DMT2. application/pdf Choi, Marcelo Puchulu, Félix Aiello, Alejandro Nefropatía diabética Ratas Diabetes Diabetes millitus tipo 2 Zucker Diabetic Fatty Heat Shock Proteins Rat spa Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ Ciencias de la vida Rol de las Heat Shock Proteins en tejido renal de ratas Zucker Diabetic Fatty info:eu-repo/semantics/doctoralThesis info:ar-repo/semantics/tesis doctoral info:eu-repo/semantics/acceptedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_2825 http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_2825.dir/2825.PDF
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
language	Español
orig_language_str_mv	spa
topic	Nefropatía diabética Ratas Diabetes Diabetes millitus tipo 2 Zucker Diabetic Fatty Heat Shock Proteins Rat Ciencias de la vida
spellingShingle	Nefropatía diabética Ratas Diabetes Diabetes millitus tipo 2 Zucker Diabetic Fatty Heat Shock Proteins Rat Ciencias de la vida Maselli, María del Carmen Rol de las Heat Shock Proteins en tejido renal de ratas Zucker Diabetic Fatty
topic_facet	Nefropatía diabética Ratas Diabetes Diabetes millitus tipo 2 Zucker Diabetic Fatty Heat Shock Proteins Rat Ciencias de la vida
description	The aim of this study was to evaluate whether the "Heat Shock Proteins" (HSP) can control functional and morphological renal damage in a recognized animal model of type 2 diabetes (DMT2). Male 12-week old "Zucker Diabetic Fatty" (ZDF) rats and control "Lean Zucker Rat¨ (LZR) were used. All animals received water and standard rat chow, "ad libitum". The immunoexpression of HSP70 and HSP27 was assessed in kidney tissue. Markers of glomerular and tubulointerstitial damage (oxidative stress, inflammation, nitrosative stress, fat deposition, apoptosis, interstitial fibrosis), and podocyte injury were analyzed. Ultrastructural glomerular alterations were evaluated by electron microscopy. We correlated biochemical variables and histological markers of podocyte damage and expression of HSPs in renal tissue. The conclusion of this study is that the increased expression of HSP70 and HSP27 found in kidney of ZDF rats, although it represents an activated repair mechanism in response to an adverse metabolic scenario, its participation does not succeed in modulating the injury caused in such tissue, showing insufficient or at least incomplete behavior due to the magnitude of renal cell damage induced in this experimental model of T2DM.
author2	Angerosa, Margarita
author_facet	Angerosa, Margarita Maselli, María del Carmen
format	Tesis doctoral Tesis doctoral acceptedVersion
author	Maselli, María del Carmen
author_sort	Maselli, María del Carmen
title	Rol de las Heat Shock Proteins en tejido renal de ratas Zucker Diabetic Fatty
title_short	Rol de las Heat Shock Proteins en tejido renal de ratas Zucker Diabetic Fatty
title_full	Rol de las Heat Shock Proteins en tejido renal de ratas Zucker Diabetic Fatty
title_fullStr	Rol de las Heat Shock Proteins en tejido renal de ratas Zucker Diabetic Fatty
title_full_unstemmed	Rol de las Heat Shock Proteins en tejido renal de ratas Zucker Diabetic Fatty
title_sort	rol de las heat shock proteins en tejido renal de ratas zucker diabetic fatty
publisher	Facultad de Farmacia y Bioquímica
publishDate	2018
url	http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_2825 http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_2825.dir/2825.PDF
work_keys_str_mv	AT masellimariadelcarmen roldelasheatshockproteinsentejidorenalderataszuckerdiabeticfatty
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Rol de las Heat Shock Proteins en tejido renal de ratas Zucker Diabetic Fatty

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