Síndrome diarreico en potrillos, diagnóstico y caracterización de los agentes virales involucrados

Neonatal diarrhea is one of the most relevant and frequent syndromes in foals younger than six month of age. Group A Rotavirus (RVA) is the main viral agent associated to diarrhea in foals, worldwide. However, around 50% of the diarrhea episodes remain without an etiological diagnosis. The aims of t...

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Autor principal: Miño, Orlando Samuel
Otros Autores: Parreño, Viviana
Formato: Tesis doctoral acceptedVersion
Lenguaje:Español
Publicado: Facultad de Ciencias Veterinarias 2017
Materias:
Acceso en línea:http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=avaposgra&cl=CL1&d=HWA_1923
http://repositoriouba.sisbi.uba.ar/gsdl/collect/avaposgra/index/assoc/HWA_1923.dir/1923.PDF
Aporte de:
id I28-R145-HWA_1923
record_format dspace
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-145
collection Repositorio Digital de la Universidad de Buenos Aires (UBA)
language Español
orig_language_str_mv spa
topic Rotavirus
Coronavirus
Norovirus
Equinos
Reoviridae
Potrillo
Síndrome diarreico
Diagnóstico
Virus
Virología
spellingShingle Rotavirus
Coronavirus
Norovirus
Equinos
Reoviridae
Potrillo
Síndrome diarreico
Diagnóstico
Virus
Virología
Miño, Orlando Samuel
Síndrome diarreico en potrillos, diagnóstico y caracterización de los agentes virales involucrados
topic_facet Rotavirus
Coronavirus
Norovirus
Equinos
Reoviridae
Potrillo
Síndrome diarreico
Diagnóstico
Virus
Virología
description Neonatal diarrhea is one of the most relevant and frequent syndromes in foals younger than six month of age. Group A Rotavirus (RVA) is the main viral agent associated to diarrhea in foals, worldwide. However, around 50% of the diarrhea episodes remain without an etiological diagnosis. The aims of the present work were, to go in detail into the molecular epidemiology, the evolutive and structural characteristics of equine RVA and to investigate the presence and role of other viral agents as potential causes of diarrhea in Argentinean foals. The study in equine RVA started with the evaluation of the sensitivity and specificity of tree diagnosis tests. Secondly, we studied the molecular epidemiology of the RVA circulating in Argentinean foals from 2009 to 2014. The G3P[12] and G14P[12] genotypes were detected with a cyclic prevalence among the years under study. Considering all the genome sequences of the equine RVA strains available an evolutive study allowed us to determine the monophyletic clustering of the equine RVA detected in Argentina. The E12 genotype -encoding the viral enterotoxin NSP4- was defined as a geographical marker which allows describing the evolution of the equine RVA present in South America since the first foundation of Buenos Aires in 1536. The obtained results led to the hypothesis that an inter-species transmission gave rise to new RVA genome constellations as a result of gene reassortment between the RVA circulating in American autochthonous species (Lama guanicoe) and the RVA of the introduced species (equine and bovine). The E12 gene from the autochthonous RVA to the equine and bovine RVA backbones were transferred and fixed. The study of equine RVA also included a structural analysis that showed the genetic linkage between the two genotypes of the VP7 and VP6 structural proteins. Finally, the virome of diarrheic samples from foals were studied by ?next generation sequencing? technology. In conclusion the present work contributed new information regarding equine RVA diagnosis and addressed aspects related to evolutive, phylodynamic and structural processes of RVA that might be extrapolated to other viruses carrying segmented genomes. In addition, the analysis of the virome from samples from diarrheic foals allowed us to detect other potential viral agents associated to diarrhea in equines. Our results remark the important of conducting the continuous surveillance of diarrhea in foals, the identification and characterization of the associated viral agents with the final goal of the rationale design of new control and preventive tools.
author2 Parreño, Viviana
author_facet Parreño, Viviana
Miño, Orlando Samuel
format Tesis doctoral
Tesis doctoral
acceptedVersion
author Miño, Orlando Samuel
author_sort Miño, Orlando Samuel
title Síndrome diarreico en potrillos, diagnóstico y caracterización de los agentes virales involucrados
title_short Síndrome diarreico en potrillos, diagnóstico y caracterización de los agentes virales involucrados
title_full Síndrome diarreico en potrillos, diagnóstico y caracterización de los agentes virales involucrados
title_fullStr Síndrome diarreico en potrillos, diagnóstico y caracterización de los agentes virales involucrados
title_full_unstemmed Síndrome diarreico en potrillos, diagnóstico y caracterización de los agentes virales involucrados
title_sort síndrome diarreico en potrillos, diagnóstico y caracterización de los agentes virales involucrados
publisher Facultad de Ciencias Veterinarias
publishDate 2017
url http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=avaposgra&cl=CL1&d=HWA_1923
http://repositoriouba.sisbi.uba.ar/gsdl/collect/avaposgra/index/assoc/HWA_1923.dir/1923.PDF
work_keys_str_mv AT minoorlandosamuel sindromediarreicoenpotrillosdiagnosticoycaracterizaciondelosagentesviralesinvolucrados
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spelling I28-R145-HWA_19232019-09-25 Neonatal diarrhea is one of the most relevant and frequent syndromes in foals younger than six month of age. Group A Rotavirus (RVA) is the main viral agent associated to diarrhea in foals, worldwide. However, around 50% of the diarrhea episodes remain without an etiological diagnosis. The aims of the present work were, to go in detail into the molecular epidemiology, the evolutive and structural characteristics of equine RVA and to investigate the presence and role of other viral agents as potential causes of diarrhea in Argentinean foals. The study in equine RVA started with the evaluation of the sensitivity and specificity of tree diagnosis tests. Secondly, we studied the molecular epidemiology of the RVA circulating in Argentinean foals from 2009 to 2014. The G3P[12] and G14P[12] genotypes were detected with a cyclic prevalence among the years under study. Considering all the genome sequences of the equine RVA strains available an evolutive study allowed us to determine the monophyletic clustering of the equine RVA detected in Argentina. The E12 genotype -encoding the viral enterotoxin NSP4- was defined as a geographical marker which allows describing the evolution of the equine RVA present in South America since the first foundation of Buenos Aires in 1536. The obtained results led to the hypothesis that an inter-species transmission gave rise to new RVA genome constellations as a result of gene reassortment between the RVA circulating in American autochthonous species (Lama guanicoe) and the RVA of the introduced species (equine and bovine). The E12 gene from the autochthonous RVA to the equine and bovine RVA backbones were transferred and fixed. The study of equine RVA also included a structural analysis that showed the genetic linkage between the two genotypes of the VP7 and VP6 structural proteins. Finally, the virome of diarrheic samples from foals were studied by ?next generation sequencing? technology. In conclusion the present work contributed new information regarding equine RVA diagnosis and addressed aspects related to evolutive, phylodynamic and structural processes of RVA that might be extrapolated to other viruses carrying segmented genomes. In addition, the analysis of the virome from samples from diarrheic foals allowed us to detect other potential viral agents associated to diarrhea in equines. Our results remark the important of conducting the continuous surveillance of diarrhea in foals, the identification and characterization of the associated viral agents with the final goal of the rationale design of new control and preventive tools. Fil: Miño, Orlando Samuel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Buenos Aires, Argentina Parreño, Viviana Facultad de Ciencias Veterinarias Barrandeguy, María Miño, Orlando Samuel 2017-08-23 Las diarreas neonatales representan uno de los síndromes clínicos más relevantes y frecuentes en potrillos menores de 6 meses de edad. Rotavirus grupo A (RVA) es el principal agente viral asociado a diarreas en potrillos, a nivel mundial. Sin embargo, en aproximadamente el 50% de los casos de diarrea no se puede establecer su etiología. Por estos motivos, los objetivos de este trabajo fueron, profundizar el conocimiento de la epidemiología molecular y, de las características evolutivas y estructurales de RVA equino, y estudiar la presencia de otros agentes virales como posibles causantes de diarreas en potrillos de nuestro país.\nEl estudio de RVA equino comenzó con la determinación de la sensibilidad y especificidad de tres metodos de diagnóstico. En segundo lugar, se estudió la epidemiología molecular de los RVA circulantes en equinos durante el período 2009 ? 2014. Las variantes G3P[12] y G14P[12] fueron las cepas detectadas con una prevalencia cíclica y alternada entre los años bajo estudio. Con todas las cepas disponibles se realizó un estudio evolutivo, determinándose la monofilia de los RVA equinos de Argentina. Se identificó el genotipo E12 -correspondiente al gen codificante de la enterotoxina viral NSP4- como un indicador geográfico que permitió trazar la evolución de los RVA equino de Sudamérica, desde la primera fundación de Buenos Aires en 1536. El resultado obtenido permite plantear la hipótesis de una infección inter especie que dio origen a una cepa emergente resultado de la reasociación de genes entre las cepas de RVA de animales autóctonos (guanacos) y las cepas de RVA de los animales introducidos (equinos y bovinos). Se detectó el fenómeno de transferencia y fijación del genotipo E12 autóctono en las cepas de RVA circulantes en equinos y bovinos de Sudamérica. El estudio de RVA equino incluyó también un análisis estructural que permitió explicar el ligamiento genético observado entre las variantes de las proteínas estructurales VP7 y VP6. Finalmente, mediante la tecnología de ?next generation sequencing?, se estudió el viroma de la materia fecal de potrillos con diarrea. Así se determinó la presencia de otros agentes virales, potenciales causantes de diarrea.En conclusión, este trabajo profundizó aspectos relacionados con el diagnóstico de RVA equino, aportando datos novedosos respecto a los procesos evolutivos, filodinámicos y estructurales que podrían extrapolarse a otros virus de genoma segmentado. Asimismo, se describió por primera vez el viroma de materia fecal de potrillos con diarrea, sugiriendo otros posibles agentes causales. Nuestros resultados enfatizan la importancia de realizar una vigilancia epidemiológica continua de las diarreas en potrillos, profundizar en la identificación y caracterización de los agentes virales asociados, con el fin último de realizar el diseño racional de nuevas herramientas de prevención y control. application/pdf Rotavirus Coronavirus Norovirus Equinos Reoviridae spa Universidad de Buenos Aires. Facultad de Ciencias Veterinarias info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ Potrillo Síndrome diarreico Diagnóstico Virus Virología Síndrome diarreico en potrillos, diagnóstico y caracterización de los agentes virales involucrados info:eu-repo/semantics/doctoralThesis info:ar-repo/semantics/tesis doctoral info:eu-repo/semantics/acceptedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=avaposgra&cl=CL1&d=HWA_1923 http://repositoriouba.sisbi.uba.ar/gsdl/collect/avaposgra/index/assoc/HWA_1923.dir/1923.PDF