InVet. 2015, 17 (2): 203-210 ISSN 1514-6634 (impreso) SEROLOGÍA DE...
Goat production is an important item in the agricultural system of Argentina and brucellosis caused by Brucella melitensis is considered the main cause reproductive problems in this species. Although definitive diagnosis for this disease is made by isolation of the etiologic agent, but in practice t...
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| Autores principales: | , , , , |
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Español |
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Universidad de Buenos Aires. Facultad de Ciencias Veterinarias
2015
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| Materias: | |
| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_1305 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_1305.dir/1305.PDF |
| Aporte de: |
| id |
I28-R145-HWA_1305 |
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| record_format |
dspace |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| language |
Español |
| orig_language_str_mv |
spa |
| topic |
Brucelosis Diagnóstico Caprinos Brucellosis Diagnosis Goats |
| spellingShingle |
Brucelosis Diagnóstico Caprinos Brucellosis Diagnosis Goats Cisterna, C. Conde, S. Hollender, D. Martino, P.E. Samartino L. InVet. 2015, 17 (2): 203-210 ISSN 1514-6634 (impreso) SEROLOGÍA DE... |
| topic_facet |
Brucelosis Diagnóstico Caprinos Brucellosis Diagnosis Goats |
| description |
Goat production is an important item in the agricultural system of Argentina and brucellosis caused by Brucella melitensis is considered the main cause reproductive problems in this species. Although definitive diagnosis for this disease is made by isolation of the etiologic agent, but in practice the health status of a herd was evaluated by serological techniques indirect testing. To evaluate and compare the performance of different serological tests for diagnosis of brucellosis in goats, serum samples were analyzed. The following tests were performed: buffered plate antigen (BPA), rose bengal (RB RB 3% and 8%), fluorescence polarization (FPA) and the test tube and 2 mercaptoethanol (SAT/2ME). The validity of each test was determined in a relative manner by combination of indirect ELISA (IELISA) and complement fixation (CF). BPA was the most sensitive test, followed by SAT/2ME, 3% RB, FPA and, finally, RB 8%. FPA test, when used in series with BPA, significantly improved overall specificity. Based on these results it was proposed for brucellosis diagnosis in goats, BPA as a screening test, FPA as complementary test, and FC and IELISA as confirmatory tests. SAT/2ME is recommended only when it is not possible to perform other tests, due to the toxic effects of SAT/2ME reagents. |
| format |
Artículo Artículo publishedVersion |
| author |
Cisterna, C. Conde, S. Hollender, D. Martino, P.E. Samartino L. |
| author_facet |
Cisterna, C. Conde, S. Hollender, D. Martino, P.E. Samartino L. |
| author_sort |
Cisterna, C. |
| title |
InVet. 2015, 17 (2): 203-210 ISSN 1514-6634 (impreso) SEROLOGÍA DE... |
| title_short |
InVet. 2015, 17 (2): 203-210 ISSN 1514-6634 (impreso) SEROLOGÍA DE... |
| title_full |
InVet. 2015, 17 (2): 203-210 ISSN 1514-6634 (impreso) SEROLOGÍA DE... |
| title_fullStr |
InVet. 2015, 17 (2): 203-210 ISSN 1514-6634 (impreso) SEROLOGÍA DE... |
| title_full_unstemmed |
InVet. 2015, 17 (2): 203-210 ISSN 1514-6634 (impreso) SEROLOGÍA DE... |
| title_sort |
invet. 2015, 17 (2): 203-210 issn 1514-6634 (impreso) serología de... |
| publisher |
Universidad de Buenos Aires. Facultad de Ciencias Veterinarias |
| publishDate |
2015 |
| url |
http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_1305 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_1305.dir/1305.PDF |
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| _version_ |
1824952393649881088 |
| spelling |
I28-R145-HWA_13052024-12-20 InVet. 2015, 17 (2): 203-210 ISSN 1514-6634 (impreso) SEROLOGÍA DE... Goat production is an important item in the agricultural system of Argentina and brucellosis caused by Brucella melitensis is considered the main cause reproductive problems in this species. Although definitive diagnosis for this disease is made by isolation of the etiologic agent, but in practice the health status of a herd was evaluated by serological techniques indirect testing. To evaluate and compare the performance of different serological tests for diagnosis of brucellosis in goats, serum samples were analyzed. The following tests were performed: buffered plate antigen (BPA), rose bengal (RB RB 3% and 8%), fluorescence polarization (FPA) and the test tube and 2 mercaptoethanol (SAT/2ME). The validity of each test was determined in a relative manner by combination of indirect ELISA (IELISA) and complement fixation (CF). BPA was the most sensitive test, followed by SAT/2ME, 3% RB, FPA and, finally, RB 8%. FPA test, when used in series with BPA, significantly improved overall specificity. Based on these results it was proposed for brucellosis diagnosis in goats, BPA as a screening test, FPA as complementary test, and FC and IELISA as confirmatory tests. SAT/2ME is recommended only when it is not possible to perform other tests, due to the toxic effects of SAT/2ME reagents. Fil: Cisterna, C. Comisión de Investigaciones Científicas (CIC). Buenos Aires, Argentina Fil: Conde, S. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología. Buenos Aires, Argentina Fil: Hollender, D. CONICET. Argentina Fil: Martino, P.E. Comisión de Investigaciones Científicas (CIC). Buenos Aires, Argentina Fil: Samartino L. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología. Buenos Aires, Argentina Cisterna, C. Conde, S. Hollender, D. Martino, P.E. Samartino L. 2015 La producción caprina representa un rubro importante dentro del sistema agropecuario de la República Argentina y la brucelosis causada por Brucella melitensis es considerada el motivo principal de los problemas reproductivos en esta especie. El diagnóstico de certeza se realiza aislando el agente etiológico, aunque en la práctica se evalúa el estado sanitario de un hato mediante pruebas serológicas. El objetivo de este trabajo fue evaluar y comparar el desempeño de las distintas pruebas serológicas tamíz y complementarias para el diagnóstico de la brucelosis caprina. Para ello, se analizaron muestras de suero de animales sin vacunación, realizando antígeno buferado en placa (BPA), rosa de bengala (RB 3 % y RB 8 %), polarización fluorescente (FPA) y pruebas de tubo y 2 mercaptoetanol (SAT/2ME). La validez de cada prueba se determinó por combinación de ELISA Indirecto (IELISA) y Fijación de Complemento (FC). BPA resultó ser más sensible, seguido de SAT/2ME, RB 3%, FPA y por último RB 8%. Al realizar FPA en serie con BPA mejoró significativamente la especificidad global. Se propone BPA como prueba tamíz, FPA como complementaria y FC e IELISA como confirmatorias. Se recomienda SAT/2ME sólo en los casos en que no sea posible la realización de las otras pruebas debido a los efectos tóxicos de los reactivos empleados para su realización. application/pdf 1514-6634 (impreso) 1668-3498 (en línea) Brucelosis Diagnóstico Caprinos Brucellosis Diagnosis Goats spa Universidad de Buenos Aires. Facultad de Ciencias Veterinarias info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ InVet, vol. 17, nº 2 Diagnóstico serológico de brucelosis en caprinos: comparación de técnicas Serological diagnosis of brucellosis in goats: comparison of techniques info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_1305 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_1305.dir/1305.PDF |