Estudio del efecto de eritropoyetina y kit ligando en la maduración y competencia in vitro de ovocitos bovinos

The overall aim of this thesis was to evaluate the effect of erythropoietin (EPO) and kit ligand (KITL) during in vitro maturation (IVM) of oocytes.\nTo study the role of both factors during IVM of oocytes we used bovine and murine models. In cattle the effect of adding EPO and KITL to maturation me...

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Autor principal: Conde, Pamela Andrea
Otros Autores: Cetica, Pablo
Formato: Tesis doctoral acceptedVersion
Lenguaje:Español
Publicado: Facultad de Farmacia y Bioquímica 2015
Materias:
Bovino
Maduración in vitro
Ovocitos
Crecimiento Hematopoyético
Ciencia de la vida
Acceso en línea:http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_1141
http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_1141.dir/1141.PDF
Aporte de:
Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires
id I28-R145-HWA_1141
record_format dspace
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-145
collection Repositorio Digital de la Universidad de Buenos Aires (UBA)
language Español
orig_language_str_mv spa
topic Bovino
Maduración in vitro
Ovocitos
Crecimiento Hematopoyético
Ciencia de la vida
spellingShingle Bovino
Maduración in vitro
Ovocitos
Crecimiento Hematopoyético
Ciencia de la vida
Conde, Pamela Andrea
Estudio del efecto de eritropoyetina y kit ligando en la maduración y competencia in vitro de ovocitos bovinos
topic_facet Bovino
Maduración in vitro
Ovocitos
Crecimiento Hematopoyético
Ciencia de la vida
description The overall aim of this thesis was to evaluate the effect of erythropoietin (EPO) and kit ligand (KITL) during in vitro maturation (IVM) of oocytes.\nTo study the role of both factors during IVM of oocytes we used bovine and murine models. In cattle the effect of adding EPO and KITL to maturation media in the presence of fetal calf serum (FCS) or bovine serumalbumin (BSA) in nuclear and cytoplasmic maturation, the production of reactive oxygen species (ROS) in oocytes and the presence of apoptosis in cumulus cells was studied. In murine the action of both factors during IVM of oocytes obtained from follicles developed in vitro was evaluated.\nAlthough no significant difference were observed in the percentage of nuclear maturation, the number of cleaved bovine embryos and blastocyst obtained increased or tended to increase respect to the control with BSA with the addition of EPO or KITL, respectively. With both factors the number of blastomeres per embryo on day 2 of development was higher, the percentage of cumulus cells undergoing apoptosis decreased and ROS production per embryo was increased compared with the control with BSA.\nIn mouse, the presence of KITL during in vitro maturation of oocytes arising from preantral follicle culture increased the percentage of blastocysts obtained. EPO showed no significant differences.\nIn conclusion, hematopoietic growth factors may have no impact on nuclear maturation of bovine or murine oocytes, however they could have a positive effect on cytoplasmic maturation and developmental competence at least in the conditions studied here.
author2 Cetica, Pablo
author_facet Cetica, Pablo
Conde, Pamela Andrea
format Tesis doctoral
Tesis doctoral
acceptedVersion
author Conde, Pamela Andrea
author_sort Conde, Pamela Andrea
title Estudio del efecto de eritropoyetina y kit ligando en la maduración y competencia in vitro de ovocitos bovinos
title_short Estudio del efecto de eritropoyetina y kit ligando en la maduración y competencia in vitro de ovocitos bovinos
title_full Estudio del efecto de eritropoyetina y kit ligando en la maduración y competencia in vitro de ovocitos bovinos
title_fullStr Estudio del efecto de eritropoyetina y kit ligando en la maduración y competencia in vitro de ovocitos bovinos
title_full_unstemmed Estudio del efecto de eritropoyetina y kit ligando en la maduración y competencia in vitro de ovocitos bovinos
title_sort estudio del efecto de eritropoyetina y kit ligando en la maduración y competencia in vitro de ovocitos bovinos
publisher Facultad de Farmacia y Bioquímica
publishDate 2015
url http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_1141
http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_1141.dir/1141.PDF
work_keys_str_mv AT condepamelaandrea estudiodelefectodeeritropoyetinaykitligandoenlamaduracionycompetenciainvitrodeovocitosbovinos
_version_ 1766017454423670784
spelling I28-R145-HWA_11412019-09-25 The overall aim of this thesis was to evaluate the effect of erythropoietin (EPO) and kit ligand (KITL) during in vitro maturation (IVM) of oocytes.\nTo study the role of both factors during IVM of oocytes we used bovine and murine models. In cattle the effect of adding EPO and KITL to maturation media in the presence of fetal calf serum (FCS) or bovine serumalbumin (BSA) in nuclear and cytoplasmic maturation, the production of reactive oxygen species (ROS) in oocytes and the presence of apoptosis in cumulus cells was studied. In murine the action of both factors during IVM of oocytes obtained from follicles developed in vitro was evaluated.\nAlthough no significant difference were observed in the percentage of nuclear maturation, the number of cleaved bovine embryos and blastocyst obtained increased or tended to increase respect to the control with BSA with the addition of EPO or KITL, respectively. With both factors the number of blastomeres per embryo on day 2 of development was higher, the percentage of cumulus cells undergoing apoptosis decreased and ROS production per embryo was increased compared with the control with BSA.\nIn mouse, the presence of KITL during in vitro maturation of oocytes arising from preantral follicle culture increased the percentage of blastocysts obtained. EPO showed no significant differences.\nIn conclusion, hematopoietic growth factors may have no impact on nuclear maturation of bovine or murine oocytes, however they could have a positive effect on cytoplasmic maturation and developmental competence at least in the conditions studied here. Fil: Conde, Pamela Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Cetica, Pablo Facultad de Farmacia y Bioquímica Gentile, Teresa Conde, Pamela Andrea 2015-04-09 El objetivo general de esta Tesis Doctoral consistió en evaluar el efecto de eritropoyetina (EPO) y kit ligando (KITL), durante la maduración in vitro (MIV) de ovocitos.\nSe utilizaron dos modelos, bovino y murino. En bovinos se estudió el efecto de EPO y KITL en el medio de MIV, en presencia de suero fetal bovino (FCS) o seroalbúmina bovina (BSA), sobre la maduración nuclear y citoplasmática del ovocito, la producción de especies reactivas del oxígeno (ROS) en ovocitos y embriones y la presencia de células apoptóticas en el cumulus. En murinos se evaluó la acción de ambos factores durante la MIV de ovocitos obtenidos a partir del cultivo in vitro de folículos.\nEn presencia de EPO o KITL, el porcentaje de embriones bovinos clivados y de blastocistos se incrementó o tendió a incrementarse respecto al control con BSA, respectivamente. No se observaron diferencias significativas en la maduración nuclear. El número de blastómeras por embrión de 48 hs. fue mayor, se observó un aumento en la producción de ROS por embrión y disminuyó significativamente el porcentaje de apoptosis en las células cumulus respecto al control con BSA.\nEn ratón, la presencia de KITL durante la MIV de ovocitos obtenidos a partir del cultivo in vitro de folículos preantrales aumentó el porcentaje de blastocistos. EPO no mostró diferencias significativas.\nEn conclusión, EPO y KITL no tendrían impacto en la maduración nuclear de los ovocitos bovinos o murinos, sin embargo podrían ejercer un efecto positivo sobre la maduración citoplasmática en las condiciones estudiadas. application/pdf Settón, Patricia Crotogini, Alberto Valcarcel, Alberto Bovino Maduración in vitro Ovocitos Crecimiento Hematopoyético spa Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ Ciencia de la vida Estudio del efecto de eritropoyetina y kit ligando en la maduración y competencia in vitro de ovocitos bovinos info:eu-repo/semantics/doctoralThesis info:ar-repo/semantics/tesis doctoral info:eu-repo/semantics/acceptedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_1141 http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_1141.dir/1141.PDF

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