Genes de resistencia a quinolonas de localización plasmídica en enterobacteriaceae

Since 1998 a number of plasmid-mediated quinolone resistance (PMQR)\ndeterminants have been reported, including the enzyme aminoglycoside\nacetyltransferase (Aac (6')-Ib-cr) variant, the efflux pumps (QepA and OqxAB),\nand the Qnr-type proteins. However, and despite numerous reports of quinolon...

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Autor principal: Rincón Cruz, Giovanna
Otros Autores: Di Conza, José
Formato: Tesis doctoral acceptedVersion
Lenguaje:Español
Publicado: Facultad de Farmacia y Bioquímica 2015
Materias:
qnrB
aac(6´)-Ib-cr
qepA
oqxAB
PMQR
Resistencia plasmídica a quinolonas
Plasmid-mediated quinolone resistance
Ciencia de la vida
Acceso en línea:http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_1132
http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_1132.dir/1132.PDF
Aporte de:
Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires
id I28-R145-HWA_1132
record_format dspace
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-145
collection Repositorio Digital de la Universidad de Buenos Aires (UBA)
language Español
orig_language_str_mv spa
topic qnrB
aac(6´)-Ib-cr
qepA
oqxAB
PMQR
Resistencia plasmídica a quinolonas
qnrB
aac(6´)-Ib-cr
qepA
oqxAB
PMQR
Plasmid-mediated quinolone resistance
Ciencia de la vida
spellingShingle qnrB
aac(6´)-Ib-cr
qepA
oqxAB
PMQR
Resistencia plasmídica a quinolonas
qnrB
aac(6´)-Ib-cr
qepA
oqxAB
PMQR
Plasmid-mediated quinolone resistance
Ciencia de la vida
Rincón Cruz, Giovanna
Genes de resistencia a quinolonas de localización plasmídica en enterobacteriaceae
topic_facet qnrB
aac(6´)-Ib-cr
qepA
oqxAB
PMQR
Resistencia plasmídica a quinolonas
qnrB
aac(6´)-Ib-cr
qepA
oqxAB
PMQR
Plasmid-mediated quinolone resistance
Ciencia de la vida
description Since 1998 a number of plasmid-mediated quinolone resistance (PMQR)\ndeterminants have been reported, including the enzyme aminoglycoside\nacetyltransferase (Aac (6')-Ib-cr) variant, the efflux pumps (QepA and OqxAB),\nand the Qnr-type proteins. However, and despite numerous reports of quinolone\nresistance, there are few studies related to PMQR.\nOur main objective was to investigate PMQR prevalence in three collections of\nEnterobacteriaceae resistant to third-generation cephalosporins (RCTG)\nisolated from clinical specimens in Argentina (A), Peru (P) and Colombia (C)\nand one Colombian collection susceptible to these antibiotics.\nThe most prevalent PMQR were aac(6')-Ib-cr (A= 38.6%, P= 55.5%, Cresistant=\n71.4% and C-susceptible= 18.7%) and qnrB (A= 36.8 %; P= 25.9 %,\nC-resistant= 52.4% and C-susceptible= 3.1 %). The qepA1 allele was found in\ntwo out of three qepA positive isolates (one from each country). The third was a\nnew allele with 99% identity to qepA1 and qepA2.\nThe oqxAB genes were detected in E. coli from Perú and they were associated\nto IS6 family insertion sequences harboring in plasmids from 90 to 100 kb.\nSignificant resistance to quinolones and fluoroquinolones was shown in RCTG\nisolates, along with a high prevalence of PMQR genes. Differences in frequency\nof PMQR were observed between RCTG and sensitive isolates. This work is the\nfirst description of prevalence of PMQR genes in RCTG isolates from Argentina,\nPeru and Colombia; moreover, it is the first description and characterization of\nthe qepA and oqxAB in clinical isolates from each origin country.
author2 Di Conza, José
author_facet Di Conza, José
Rincón Cruz, Giovanna
format Tesis doctoral
Tesis doctoral
acceptedVersion
author Rincón Cruz, Giovanna
author_sort Rincón Cruz, Giovanna
title Genes de resistencia a quinolonas de localización plasmídica en enterobacteriaceae
title_short Genes de resistencia a quinolonas de localización plasmídica en enterobacteriaceae
title_full Genes de resistencia a quinolonas de localización plasmídica en enterobacteriaceae
title_fullStr Genes de resistencia a quinolonas de localización plasmídica en enterobacteriaceae
title_full_unstemmed Genes de resistencia a quinolonas de localización plasmídica en enterobacteriaceae
title_sort genes de resistencia a quinolonas de localización plasmídica en enterobacteriaceae
publisher Facultad de Farmacia y Bioquímica
publishDate 2015
url http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_1132
http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_1132.dir/1132.PDF
work_keys_str_mv AT rinconcruzgiovanna genesderesistenciaaquinolonasdelocalizacionplasmidicaenenterobacteriaceae
_version_ 1766017452973490176
spelling I28-R145-HWA_11322019-09-25 Since 1998 a number of plasmid-mediated quinolone resistance (PMQR)\ndeterminants have been reported, including the enzyme aminoglycoside\nacetyltransferase (Aac (6')-Ib-cr) variant, the efflux pumps (QepA and OqxAB),\nand the Qnr-type proteins. However, and despite numerous reports of quinolone\nresistance, there are few studies related to PMQR.\nOur main objective was to investigate PMQR prevalence in three collections of\nEnterobacteriaceae resistant to third-generation cephalosporins (RCTG)\nisolated from clinical specimens in Argentina (A), Peru (P) and Colombia (C)\nand one Colombian collection susceptible to these antibiotics.\nThe most prevalent PMQR were aac(6')-Ib-cr (A= 38.6%, P= 55.5%, Cresistant=\n71.4% and C-susceptible= 18.7%) and qnrB (A= 36.8 %; P= 25.9 %,\nC-resistant= 52.4% and C-susceptible= 3.1 %). The qepA1 allele was found in\ntwo out of three qepA positive isolates (one from each country). The third was a\nnew allele with 99% identity to qepA1 and qepA2.\nThe oqxAB genes were detected in E. coli from Perú and they were associated\nto IS6 family insertion sequences harboring in plasmids from 90 to 100 kb.\nSignificant resistance to quinolones and fluoroquinolones was shown in RCTG\nisolates, along with a high prevalence of PMQR genes. Differences in frequency\nof PMQR were observed between RCTG and sensitive isolates. This work is the\nfirst description of prevalence of PMQR genes in RCTG isolates from Argentina,\nPeru and Colombia; moreover, it is the first description and characterization of\nthe qepA and oqxAB in clinical isolates from each origin country. Fil: Rincón Cruz, Giovanna. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Di Conza, José Facultad de Farmacia y Bioquímica Gutkind, Gabriel Rincón Cruz, Giovanna 2015-03-26 Si bien desde 1998 se conocieron los primeros marcadores de resistencia a quinolonas de codificación plasmídica (PMQR), y sumado a la elevada resistencia a quinolonas reportada, son escasos los estudios epidemiológicos acerca de los PMQR. Dentro de estos determinantes se describieron las proteínas tipo Qnr, la variante de la enzima acetiltransferasa (Aac(6´)-Ib-cr), y las bombas de eflujo (QepA y OqxAB).\nEl objetivo de este estudio fue investigar la frecuencia de los determinantes de resistencia a quinolonas mediados por plásmidos (PMQR) en tres colecciones de Enterobacteriaceae resistentes a cefalosporinas de tercera generación (RCTG) aisladas de especímenes clínicos de Argentina (A), Perú (P) y Colombia (C). Se incluyó, además, otra colección de aislamientos de Colombia sensible a estos antibióticos.\nLos mecanismos más frecuentemente encontrados fueron aac(6´)-Ib-cr (A= 38,6 %, P= 55,5 %, C-resistente= 71,4 % y C-sensible= 18,7 %) y qnrB (A= 36,8 %, P= 25,9 %,C- resistente 52,4 % y C-sensible 3,1 %). Un aislamiento por país mostró el gen qepA, de los cuales dos fueron qepA1 y el tercero un nuevo alelo con 99 % de identidad con qepA1 y qepA2.\nLos genes oqxAB se encontraron en tres aislamientos de E. coli de Perú asociados a secuencias de inserción de la familia IS6 en plásmidos de 90 a 100 Kb.\nEl trabajo muestra la importante asociación de la resistencia a fluoroquinolonas (y de las PMQR) en aislamientos RCTG, y su mayor prevalencia que en la colección sensible a oxiiminocefalosporinas. Este trabajo es la primera descripción de la prevalencia de genes de tipo PMQR en aislamiento RCTG en poblaciones de Argentina, Perú y Colombia, así como la primera descripción y caracterización de qepA y oqxAB en aislamientos clínicos de sus países de origen. application/pdf Rodríguez, Hernán Morbidoni, Ricardo Quiroga, Cecilia qnrB aac(6´)-Ib-cr qepA oqxAB PMQR Resistencia plasmídica a quinolonas qnrB aac(6´)-Ib-cr qepA oqxAB PMQR Plasmid-mediated quinolone resistance spa Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ Ciencia de la vida Genes de resistencia a quinolonas de localización plasmídica en enterobacteriaceae info:eu-repo/semantics/doctoralThesis info:ar-repo/semantics/tesis doctoral info:eu-repo/semantics/acceptedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_1132 http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_1132.dir/1132.PDF

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