Caracterización molecular de los integrones de clase1 en Protus mirabilis
According to the data published by the CDC, there are 90.000 deaths per year in the US due to nosocomial infections. In recent years people talks about modern evolution, where not only come into play mutations as a major genetic mechanism to evolve and to make bacteria antimicrobial resistant, but a...
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Facultad de Farmacia y Bioquímica
2015
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=afamaster&cl=CL1&d=HWA_1127 http://repositoriouba.sisbi.uba.ar/gsdl/collect/afamaster/index/assoc/HWA_1127.dir/1127.PDF |
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I28-R145-HWA_1127 |
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dspace |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
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Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| language |
Español |
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spa |
| topic |
Integrones Género proteus Resistencia Antibiótica Proteus mirabilis Ciencia de la vida |
| spellingShingle |
Integrones Género proteus Resistencia Antibiótica Proteus mirabilis Ciencia de la vida Turina, Maria Adelaida Caracterización molecular de los integrones de clase1 en Protus mirabilis |
| topic_facet |
Integrones Género proteus Resistencia Antibiótica Proteus mirabilis Ciencia de la vida |
| description |
According to the data published by the CDC, there are 90.000 deaths per year in the US due to nosocomial infections. In recent years people talks about modern evolution, where not only come into play mutations as a major genetic mechanism to evolve and to make bacteria antimicrobial resistant, but also have revealed other mechanisms such as Lateral Genetic Transfer (LGT), which is a complex, multi-factorial and important mechanism in the evolution of various agents not only in bacteria. Antimicrobial resistance is the best example of the pressure-selection and genomic and phenotypic adaptation process based in LGT as approximately 80% of the resistances is due to LGT. By 1980, genetic elements associated to the acquisition and loss of genes were observed, which were identified as integrons. The current definition of integron defines it as an element that contains the genetic determinants to mediate site-specific recombination, being able to recognize and capture mobile genes cassettes, many of which encode determinants of antibiotic resistance.\nOur goal is to know the frequency of class 1 integrons and the gene cassette arrays in P. mirabilis as well as the evolution over 15 years into a collection of epidemiologically unrelated strains that are resistant to at least two antimicrobial families.\nThe study was conducted in 35 epidemiologically non related isolates of P. mirabilis from five hospitals of the Autonomous City of Buenos Aires and Buenos Aires province, isolated between 1993 and 2008. The selection criterion was that the isolates should be resistant to at least two antimicrobial families. It was determined the susceptibility of the studied isolates to eleven antimicrobials of clinical relevance by the agar disk diffusion method according to the guidelines of the National Committee for\nCaracterización Molecular del los Integrones de clase 1 en Proteus mirabilis\nBqca. M. A. TURINA|\nClinical Laboratory Standards (CLSI, 2013). To carry out the characterization of class 1 integrons, PCR mapping was performed by using different combinations of primers to characterize the structure and content of gene cassettes in the variable region.\nThe 91.43% of the isolates were resistant to ampicillin. Resistance to ceftazidime, cefotaxime and cefepime was evidenced in 48.57% of total isolates that had phenotype of CTX-M-2 extended spectrum ?-lactamase (ESBL). All the isolates were susceptibility to carbapenems. The 31.43% of the isolates were resistant to gentamicin, while 17.14% had intermediate susceptibility. Amikacin resistance was manifested in 91.43% of the isolates, 2.86% had intermediate susceptibility. Ciprofloxacin and nalidixic acid resistance in the studied isolates were 54.29% and 62.86% respectively. The percentage of resistance to trimethoprim / sulfamethoxazole was 91.43%.\nClass 1 integrons were found in 35/35 (100%) of the isolates, and ISCR1 was detected in 17/35 (48.57%) of them. 64 class 1 integrons were found in total in the 35 isolates, including 12 arrangements of different gene cassettes in their variable region, which include the combination of 9 genes cassettes, 8 of which correspond to antimicrobial resistant genes. The integrons found contain in their variable region an unfixed number of genes cassette. The arrangement of the most frequent genes cassette in the 35 isolates tested was aac (6 ') - Ib- blaOXA-2-orfD both in class 1complex and no complex integrons. Relating genes cassette arrangements of class 1 integrons with the results of antimicrobial susceptibility tested, it was observed that all isolates resistant to ceftazidime, cefotaxime and cefepime 17/35 (48.57%) have class 1 complex integrons that harbour ISCR1 with the blaCTX-M-2 gene in the VR-2.\nCaracterización Molecular del los Integrones de clase 1 en Proteus mirabilis\nBqca. M. A. TURINA|\nFrom a bioinformatic study, we observed that over a period of 20 years (1993-2013) 30 isolates of P. mirabilis with class 1 integrons were published in the Integrall database, in which 3 have class 1 complex integrons and that they are alone as such or as part of other structures such as genomic islands, plasmids and transposons. 34 class 1 integrons were found in them, with 27 arrangements of different genes cassette in their variable region, including the combination of 29 genes cassette, of which 25 correspond to antimicrobial resistant genes.\nIt was found in this collection of P. mirabilis from clinical isolates a high frequency of class 1 integrons 35/35 (100%) and ISCR1 was detected in 17/35 (48.57%) of them.\nWithin the complex class 1 integrons, In35::ISCR1::blaCTX-M-2 was the most frequent.\nIn the studied isolates for the period 1993-2008 although the amount of integrons by strain does not increase over the time with respect to the first isolates, it is observed a tendency to increase the number of families of antimicrobials that these isolates are resistant. Furthermore, the number of families of antimicrobial to which each strain is resistant depends on the gene cassette harbored in the integron structure more than the integrons number contained in the strain.\nOur results revealed once again that the LGT and with it the resistance to multiple antimicrobial families, is no longer exclusive patrimony of certain strains in particular. |
| author2 |
Quiroga, María Paula |
| author_facet |
Quiroga, María Paula Turina, Maria Adelaida |
| format |
Tesis de maestría Tesis de maestría acceptedVersion |
| author |
Turina, Maria Adelaida |
| author_sort |
Turina, Maria Adelaida |
| title |
Caracterización molecular de los integrones de clase1 en Protus mirabilis |
| title_short |
Caracterización molecular de los integrones de clase1 en Protus mirabilis |
| title_full |
Caracterización molecular de los integrones de clase1 en Protus mirabilis |
| title_fullStr |
Caracterización molecular de los integrones de clase1 en Protus mirabilis |
| title_full_unstemmed |
Caracterización molecular de los integrones de clase1 en Protus mirabilis |
| title_sort |
caracterización molecular de los integrones de clase1 en protus mirabilis |
| publisher |
Facultad de Farmacia y Bioquímica |
| publishDate |
2015 |
| url |
http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=afamaster&cl=CL1&d=HWA_1127 http://repositoriouba.sisbi.uba.ar/gsdl/collect/afamaster/index/assoc/HWA_1127.dir/1127.PDF |
| work_keys_str_mv |
AT turinamariaadelaida caracterizacionmoleculardelosintegronesdeclase1enprotusmirabilis |
| _version_ |
1766017452062277632 |
| spelling |
I28-R145-HWA_11272019-09-27 According to the data published by the CDC, there are 90.000 deaths per year in the US due to nosocomial infections. In recent years people talks about modern evolution, where not only come into play mutations as a major genetic mechanism to evolve and to make bacteria antimicrobial resistant, but also have revealed other mechanisms such as Lateral Genetic Transfer (LGT), which is a complex, multi-factorial and important mechanism in the evolution of various agents not only in bacteria. Antimicrobial resistance is the best example of the pressure-selection and genomic and phenotypic adaptation process based in LGT as approximately 80% of the resistances is due to LGT. By 1980, genetic elements associated to the acquisition and loss of genes were observed, which were identified as integrons. The current definition of integron defines it as an element that contains the genetic determinants to mediate site-specific recombination, being able to recognize and capture mobile genes cassettes, many of which encode determinants of antibiotic resistance.\nOur goal is to know the frequency of class 1 integrons and the gene cassette arrays in P. mirabilis as well as the evolution over 15 years into a collection of epidemiologically unrelated strains that are resistant to at least two antimicrobial families.\nThe study was conducted in 35 epidemiologically non related isolates of P. mirabilis from five hospitals of the Autonomous City of Buenos Aires and Buenos Aires province, isolated between 1993 and 2008. The selection criterion was that the isolates should be resistant to at least two antimicrobial families. It was determined the susceptibility of the studied isolates to eleven antimicrobials of clinical relevance by the agar disk diffusion method according to the guidelines of the National Committee for\nCaracterización Molecular del los Integrones de clase 1 en Proteus mirabilis\nBqca. M. A. TURINA|\nClinical Laboratory Standards (CLSI, 2013). To carry out the characterization of class 1 integrons, PCR mapping was performed by using different combinations of primers to characterize the structure and content of gene cassettes in the variable region.\nThe 91.43% of the isolates were resistant to ampicillin. Resistance to ceftazidime, cefotaxime and cefepime was evidenced in 48.57% of total isolates that had phenotype of CTX-M-2 extended spectrum ?-lactamase (ESBL). All the isolates were susceptibility to carbapenems. The 31.43% of the isolates were resistant to gentamicin, while 17.14% had intermediate susceptibility. Amikacin resistance was manifested in 91.43% of the isolates, 2.86% had intermediate susceptibility. Ciprofloxacin and nalidixic acid resistance in the studied isolates were 54.29% and 62.86% respectively. The percentage of resistance to trimethoprim / sulfamethoxazole was 91.43%.\nClass 1 integrons were found in 35/35 (100%) of the isolates, and ISCR1 was detected in 17/35 (48.57%) of them. 64 class 1 integrons were found in total in the 35 isolates, including 12 arrangements of different gene cassettes in their variable region, which include the combination of 9 genes cassettes, 8 of which correspond to antimicrobial resistant genes. The integrons found contain in their variable region an unfixed number of genes cassette. The arrangement of the most frequent genes cassette in the 35 isolates tested was aac (6 ') - Ib- blaOXA-2-orfD both in class 1complex and no complex integrons. Relating genes cassette arrangements of class 1 integrons with the results of antimicrobial susceptibility tested, it was observed that all isolates resistant to ceftazidime, cefotaxime and cefepime 17/35 (48.57%) have class 1 complex integrons that harbour ISCR1 with the blaCTX-M-2 gene in the VR-2.\nCaracterización Molecular del los Integrones de clase 1 en Proteus mirabilis\nBqca. M. A. TURINA|\nFrom a bioinformatic study, we observed that over a period of 20 years (1993-2013) 30 isolates of P. mirabilis with class 1 integrons were published in the Integrall database, in which 3 have class 1 complex integrons and that they are alone as such or as part of other structures such as genomic islands, plasmids and transposons. 34 class 1 integrons were found in them, with 27 arrangements of different genes cassette in their variable region, including the combination of 29 genes cassette, of which 25 correspond to antimicrobial resistant genes.\nIt was found in this collection of P. mirabilis from clinical isolates a high frequency of class 1 integrons 35/35 (100%) and ISCR1 was detected in 17/35 (48.57%) of them.\nWithin the complex class 1 integrons, In35::ISCR1::blaCTX-M-2 was the most frequent.\nIn the studied isolates for the period 1993-2008 although the amount of integrons by strain does not increase over the time with respect to the first isolates, it is observed a tendency to increase the number of families of antimicrobials that these isolates are resistant. Furthermore, the number of families of antimicrobial to which each strain is resistant depends on the gene cassette harbored in the integron structure more than the integrons number contained in the strain.\nOur results revealed once again that the LGT and with it the resistance to multiple antimicrobial families, is no longer exclusive patrimony of certain strains in particular. Fil: Turina, María Adelaida. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Quiroga, María Paula Facultad de Farmacia y Bioquímica Centrón, Daniela Turina, Maria Adelaida 2015-09-01 Según datos publicados por el C.D.C. ocurren en EEUU 90.0000 muertes por año debidas a infecciones intrahospitalarias. En los últimos años se habla de evolución moderna, donde no sólo entran en juego las mutaciones como importante mecanismo genético para evolucionar y hacer a las bacterias resistentes a los antimicrobianos, sino que además se han puesto en evidencia otros mecanismos como la Transferencia Horizontal Genética (THG), el cual es un mecanismo complejo, multifactorial, e importante en la evolución de distintos organismos. La resistencia a los antimicrobianos es el mejor ejemplo del proceso de presión-selección y adaptación genómica y fenotípica basada en la THG, ya que aproximadamente el 80% de las resistencias son debidas a este mecanismo. Hacia 1980, se observaron elementos genéticos asociados a la adquisición y pérdida de genes, los que fueron identificados como integrones. La definición actual de integrón define al integrón como un elemento que contiene los determinantes genéticos para mediar la recombinación sitio-específica, siendo capaz de reconocer y capturar genes cassettes móviles, muchos de los cuales codifican determinantes de resistencia a antibióticos.\nNuestro objetivo es conocer la frecuencia de integrones de clase 1 y los arreglos de genes cassettes en P. mirabilis así como también la evolución, a lo largo de 15 años en una colección de cepas no relacionadas epidemiológicamente que presentan al menos resistencia a dos familias de antimicrobianos.\nCaracterización Molecular del los Integrones de clase 1 en Proteus mirabilis\nBioq. M.A TURINA |\n3\nSe realizó el estudio en 35 aislamientos no relacionados epidemiológicamente de P. mirabilis provenientes de cinco nosocomios de la Ciudad Autónoma de Buenos Aires y de la Provincia de Buenos Aires, aislados entre los años 1993-2008. El criterio de selección utilizado fue que los aislamientos debían ser resistentes al menos a dos familias de antimicrobianos. Se determinó la susceptibilidad de los aislamientos en estudio a once antimicrobianos de relevancia clínica por el método de difusión en agar de acuerdo a las guías del National Committee for Clinical Laboratory Standars (CLSI, 2013). Para realizar la caracterización de los integrones de clase 1 se llevó a cabo cartografía por PCR, utilizando distintas combinaciones de cebadores a fin de caracterizar la estructura y el contenido de genes cassettes en la zona variable.\nEl 91,43% de los aislamientos fue resistente a ampicilina. La resistencia a ceftazidima, cefotaxima y cefepime se evidenció en el 48,57% del total de los aislamientos, los cuales todos presentaban fenotipo de ?-lactamasa de espectro extendido (BLEE) tipo CTX-M-2. El 100% de los aislamientos fueron sensibles a carbapenemes. El 31,43% de los aislamientos fue resistente a gentamicina, mientras que el 17,14% presentó sensibilidad intermedia. La resistencia a amikacina se manifestó en el 91,43% de los aislamientos, el 2,86% presentó sensibilidad intermedia. La resistencia a ciprofloxacina y ácido nalidíxico en los aislamientos estudiados fue de 54,29% y 62,86%, respectivamente. El porcentaje de resistencia a trimetoprima/sulfametoxazol de los mismos fue de 91,43%.\nSe encontró integrones de clase 1 en 35/35 (100%) de los aislamientos, y se detectó a ISCR1 en 17/35 (48,57%) de los mismos. Se encontraron en total 64 integrones de clase 1 en los 35 aislamientos, entre los que se distinguieron 12 arreglos de genes cassettes diferentes en su zona variable, que incluyen la combinación de 9 genes cassettes, 8 de los\nCaracterización Molecular del los Integrones de clase 1 en Proteus mirabilis\nBioq. M.A TURINA |\n4\ncuales corresponden a genes de resistencia a antimicrobianos. El arreglo de genes cassettes más frecuente en los 35 aislamientos evaluados fue aac(6?)-Ib- blaOXA-2-orfD, tanto en los integrones clase 1 complejos y no complejos, correspondiente a In35. Al relacionar los arreglos de genes cassettes de los integrones de clase 1 con los resultados de sensibilidad a los antimicrobianos ensayados, se observa que todos los aislamientos resistentes a ceftazidima, cefotaxima y cefepime 17/35 (48,57%), tienen integrones de clase 1 complejos que albergan a ISCR1 con el gen blaCTX?M-2 en la VR-2.\nAl realizar un estudio bioinformático, observamos que en un período de 20 años (1993-2013) se publicaron 30 aislamientos de P. mirabilis con integrones de clase 1 en la base de datos INTEGRALL, dentro de los cuales 3 son integrones de clase 1 complejos. Estos integrones se encuentran formando parte de otras estructuras como islas genómicas, plásmidos y transposones. En ellos se encontraron 34 integrones de clase 1, con 27 arreglos de genes cassettes diferentes en su zona variable, que incluyen la combinación de 29 genes cassettes, de los cuales 25 corresponden a genes de resistencia a antimicrobianos.\nSe encontró en esta colección de P mirabilis provenientes de aislamientos clínicos una elevada frecuencia de integrones de clase 1 35/35 (100%) y se detectó a ISCR1, en 17/35 (48,57%) de los mismos. Dentro de los integrones de clase 1 complejos, In35::ISCR1:: blaCTX?M-2 fue el más frecuente.\nEn los aislamientos estudiados correspondientes al período 1993-2008 aunque la cantidad de integrones por cepa no aumenta a lo largo del tiempo con respecto a los primeros aislamientos, se observa una tendencia a aumentar el número de familias de antimicrobianos a los que estos aislamientos presentan resistencia. Además, el número de\nCaracterización Molecular del los Integrones de clase 1 en Proteus mirabilis\nBioq. M.A TURINA |\n5\nfamilias de antimicrobianos a los que cada cepa es resistente depende de los genes cassettes que conforman la estructura del integrón más que del número de integrones contenidos en la cepa.\nNuestros resultados muestran una vez más que la THG, y junto a ella, la resistencia a múltiples familias de antimicrobianos ya no es patrimonio de ciertas cepas en particular application/pdf Almuzara, Marisa Gutkind, Gabriel Power, Pablo Integrones Género proteus Resistencia Antibiótica Proteus mirabilis spa Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ Ciencia de la vida Caracterización molecular de los integrones de clase1 en Protus mirabilis info:eu-repo/semantics/masterThesis info:ar-repo/semantics/tesis de maestría info:eu-repo/semantics/acceptedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=afamaster&cl=CL1&d=HWA_1127 http://repositoriouba.sisbi.uba.ar/gsdl/collect/afamaster/index/assoc/HWA_1127.dir/1127.PDF |