Adiposidad visceral y resistencia insulínica: rol de la AMPK
Introduction: the adipose tissue dysfunction constitutes a central point in the development of complications associated with the Metabolic Syndrome. AMP-activated protein kinase (AMPK) is considered a cellular metabolic sensor, with an important role in the regulation of the metabolic activity of wh...
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Facultad de Bioquímica y Ciencias Biológicas (UNL)
2020
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| Acceso en línea: | https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FABICIB/article/view/8367 |
| Aporte de: |
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I26-R133-article-8367 |
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| record_format |
ojs |
| institution |
Universidad Nacional del Litoral |
| institution_str |
I-26 |
| repository_str |
R-133 |
| container_title_str |
Biblioteca Virtual - Publicaciones (UNL) |
| language |
Español |
| format |
Artículo revista |
| topic |
sucrose-rich diet visceral adiposity insulin resistance AMPK dieta rica en sacarosa adiposidad visceral resistencia insulínica AMPK |
| spellingShingle |
sucrose-rich diet visceral adiposity insulin resistance AMPK dieta rica en sacarosa adiposidad visceral resistencia insulínica AMPK Vega Joubert, Michelle Oliva, María Eugenia Ferreira, Maria del Rosario D'Alessandro, Maria Eugenia Adiposidad visceral y resistencia insulínica: rol de la AMPK |
| topic_facet |
sucrose-rich diet visceral adiposity insulin resistance AMPK dieta rica en sacarosa adiposidad visceral resistencia insulínica AMPK |
| author |
Vega Joubert, Michelle Oliva, María Eugenia Ferreira, Maria del Rosario D'Alessandro, Maria Eugenia |
| author_facet |
Vega Joubert, Michelle Oliva, María Eugenia Ferreira, Maria del Rosario D'Alessandro, Maria Eugenia |
| author_sort |
Vega Joubert, Michelle |
| title |
Adiposidad visceral y resistencia insulínica: rol de la AMPK |
| title_short |
Adiposidad visceral y resistencia insulínica: rol de la AMPK |
| title_full |
Adiposidad visceral y resistencia insulínica: rol de la AMPK |
| title_fullStr |
Adiposidad visceral y resistencia insulínica: rol de la AMPK |
| title_full_unstemmed |
Adiposidad visceral y resistencia insulínica: rol de la AMPK |
| title_sort |
adiposidad visceral y resistencia insulínica: rol de la ampk |
| description |
Introduction: the adipose tissue dysfunction constitutes a central point in the development of complications associated with the Metabolic Syndrome. AMP-activated protein kinase (AMPK) is considered a cellular metabolic sensor, with an important role in the regulation of the metabolic activity of white adipose tissue.
Objectives: to evaluate the effects of the chronic administration of a sucrose rich diet on some mechanisms involved in adipose tissue dysfunction and insulin resistance.
Materials and methods: male Wistar rats received a reference diet (DR) or a sucrose-rich diet (SRD) for six months. At the end of the experimental period, the anthropometric parameters and visceral adiposity index were analyzed. In epididymal adipose tissue: triglyceride content, carnitine palmitoyl transferase enzymes activities (CPT1, CPT2 and totalCPT) and protein mass levels of AMPK and AMPKp. Serum levels of triglycerides, free fatty acid, glucose, insulin and TNFα were determined. Insulin sensitivity was measured by the euglycemic-hyperinsulinemic clamp technique.
Results: in insulin resistant dyslipidemic rats fed with SRD presented a significant increase in anthropometric parameters (body weight, CT, CA1 and 2), BMI and visceral adiposity index. In the epididymal adipose tissue, an increase in the content of triglycerides was observed, without changes in the transport of fatty acids (estimated by the measurement of the CPT activity). In addition, a decrease in the protein mass of the phosphorylated AMPK was observed that correlated with the high serum levels of TNFα.
Conclusions: the findings provide new mechanisms that involve AMPK in visceral adipose tissue dysfunction in insulin-resistant dyslipidemic rats. |
| publisher |
Facultad de Bioquímica y Ciencias Biológicas (UNL) |
| publishDate |
2020 |
| url |
https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FABICIB/article/view/8367 |
| work_keys_str_mv |
AT vegajoubertmichelle adiposidadvisceralyresistenciainsulinicaroldelaampk AT olivamariaeugenia adiposidadvisceralyresistenciainsulinicaroldelaampk AT ferreiramariadelrosario adiposidadvisceralyresistenciainsulinicaroldelaampk AT dalessandromariaeugenia adiposidadvisceralyresistenciainsulinicaroldelaampk |
| first_indexed |
2023-07-05T23:02:27Z |
| last_indexed |
2023-07-05T23:02:27Z |
| _version_ |
1770623490718695424 |
| spelling |
I26-R133-article-83672020-05-11T16:11:10Z Adiposidad visceral y resistencia insulínica: rol de la AMPK Vega Joubert, Michelle Oliva, María Eugenia Ferreira, Maria del Rosario D'Alessandro, Maria Eugenia sucrose-rich diet visceral adiposity insulin resistance AMPK dieta rica en sacarosa adiposidad visceral resistencia insulínica AMPK Introduction: the adipose tissue dysfunction constitutes a central point in the development of complications associated with the Metabolic Syndrome. AMP-activated protein kinase (AMPK) is considered a cellular metabolic sensor, with an important role in the regulation of the metabolic activity of white adipose tissue. Objectives: to evaluate the effects of the chronic administration of a sucrose rich diet on some mechanisms involved in adipose tissue dysfunction and insulin resistance. Materials and methods: male Wistar rats received a reference diet (DR) or a sucrose-rich diet (SRD) for six months. At the end of the experimental period, the anthropometric parameters and visceral adiposity index were analyzed. In epididymal adipose tissue: triglyceride content, carnitine palmitoyl transferase enzymes activities (CPT1, CPT2 and totalCPT) and protein mass levels of AMPK and AMPKp. Serum levels of triglycerides, free fatty acid, glucose, insulin and TNFα were determined. Insulin sensitivity was measured by the euglycemic-hyperinsulinemic clamp technique. Results: in insulin resistant dyslipidemic rats fed with SRD presented a significant increase in anthropometric parameters (body weight, CT, CA1 and 2), BMI and visceral adiposity index. In the epididymal adipose tissue, an increase in the content of triglycerides was observed, without changes in the transport of fatty acids (estimated by the measurement of the CPT activity). In addition, a decrease in the protein mass of the phosphorylated AMPK was observed that correlated with the high serum levels of TNFα. Conclusions: the findings provide new mechanisms that involve AMPK in visceral adipose tissue dysfunction in insulin-resistant dyslipidemic rats. Introducción: la disfunción del tejido adiposo constituye un punto central en el desarrollo de la resistencia insulínica y otras alteraciones asociadas al Síndrome Metabólico. La proteína quinasa activada por AMP (AMPK) es considerada un sensor metabólico celular, con un importante rol en la regulación de la actividad metabólica del tejido adiposo blanco. Objetivos: evaluar los efectos de la administración crónica de una dieta rica en sacarosa sobre algunos mecanismos involucrados en la disfunción del tejido adiposo y su relación con la resistencia insulínica. Materiales y métodos: ratas macho Wistar recibieron una dieta de referencia (DR) o una dieta rica en sacarosa (DRS) durante seis meses. Al final del período experimental se analizaron parámetros antropométricos e índice de adiposidad visceral. En tejido adiposo epididimal se midió: contenido de triglicéridos, actividad enzimática carnitina palmitoil transferasa (CPT1, CPT2 y CPTtotal), ácidos grasos no esterificados, glucosa, insulina y TNFα. La sensibilidad insulínica se midió por la técnica clamp euglucémica-hiperinsulinémica. Resultados: los animales dislipémicos insulinoresistentes alimentados con DRS presentaron un aumento significativo en los parámetros antropométricos peso corporal, circunferencias toráxica y abdominal e indíce de masa corporal y de adiposidad visceral. En el tejido adiposo epididimal, se observó un aumento en el contenido de triglicéridos, sin modificaciones en el transporte de ácidos grasos hacia la mitocondria (estimado por la medida de la actividad CPT). Además, se observó una disminución en la masa proteica de la AMPK fosforilada que se correlacionó con altos niveles séricos de TNFα. Conclusiones: los hallazgos aportan nuevos mecanismos que involucran a la AMPK en la disfunción del tejido adiposo visceral en ratas dislipémicas insulinorresistentes. Facultad de Bioquímica y Ciencias Biológicas (UNL) 2020-03-10 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FABICIB/article/view/8367 10.14409/fabicib.v23i0.8367 FABICIB; Vol. 23 (2019): FABICIB; 29-44 FABICIB; Vol. 23 (2019): FABICIB; 29-44 2362-5546 0329-5559 10.14409/fabicib.v23i0 spa https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FABICIB/article/view/8367/12469 Derechos de autor 2020 FABICIB |