Effect of persister cells on the efficacy of enrofloxacin and ciprofloxacin against a strain of Escherichia coli

This work evaluated the effect of persister cells present in a high inocula size of a wild strain of Escherichia coli on the efficacy of enrofloxacin and ciprofloxacin by in vitro time-kill curve assays and mutant prevention concentration testing. In time-kill curves performed with high inocula size...

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Autores principales: PATRICELLI, Paula, RAMIREZ, Eliana, PRESA ROSSA, Candela, DELL'ELCE, Antonella, FORMENTINI, Enrique
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional del Litoral 2017
Materias:
Escherichia coli
persister cells
mutant prevention
bacterias persistentes
concentración preventiva de mutantes
Acceso en línea:https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/6648
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Biblioteca Virtual - Publicaciones (UNL) de Universidad Nacional del Litoral
id I26-R133-article-6648
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spelling I26-R133-article-66482023-09-15T10:31:44Z Effect of persister cells on the efficacy of enrofloxacin and ciprofloxacin against a strain of Escherichia coli Efecto de la persistencia bacteriana sobre la eficacia de la enrofloxacina y ciprofloxacina frente a una cepa de Escherichia coli PATRICELLI, Paula RAMIREZ, Eliana PRESA ROSSA, Candela DELL'ELCE, Antonella FORMENTINI, Enrique Escherichia coli persister cells mutant prevention Escherichia coli bacterias persistentes concentración preventiva de mutantes This work evaluated the effect of persister cells present in a high inocula size of a wild strain of Escherichia coli on the efficacy of enrofloxacin and ciprofloxacin by in vitro time-kill curve assays and mutant prevention concentration testing. In time-kill curves performed with high inocula size, no antibiotics showed bactericidal activity, but only allowed the survival of persister cells. In the assay to determine the mutant prevention concentration, on the surface of agar plates containing high enrofloxacin and ciprofloxacin concentrations, persister cells remained viable and without bacterial colonies development and adopting filamentous morphology as a form of adaptation and survival. The clinical usefulness of mutant prevention concentrations of enrofloxacin and ciproflocxacin against Escherichia coli is discussed, as these high concentrations would allow the survival of a sub-population of persister cells originating a biological reservoir that could give rise to chronic infections and favor the emergence of resistant mutants. En este trabajo se evaluó el efecto de las bacterias persistentes presentes en un inóculo de alta densidad de una cepa autóctona de Escherichia coli sobre la eficacia de enrofloxacina y ciprofloxacina mediante ensayos in vitro de curvas de muerte bacteriana y de determinación de la concentración preventiva de mutantes. En las curvas de muerte realizadas sobre inóculos de alta densidad, ningún antibiótico presentó actividad bactericida y solo permitieron la sobrevida de bacterias persistentes. En el ensayo para determinar la concentración preventiva de mutantes, sobre la superficie del agar de las placas con elevadas concentraciones de enrofloxacina y ciprofloxacina, las bacterias persistentes permanecieron viables sin desarrollar colonias y adoptando morfología filamentosa como una forma de adaptación y supervivencia. Se discute la utilidad clínica de las concentraciones preventivas de mutantes de enrofloxacina y ciprofloxacina sobre E. coli ya que, estas elevadas concentraciones permitirían la sobrevida de una sub-población de bacterias persistentes originando un reservorio biológico que podría dar origen a infecciones crónicas y a favorecer la emergencia de mutantes resistentes. Universidad Nacional del Litoral 2017-07-05 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artículo original Original article application/pdf https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/6648 10.14409/favecv.v16i1.6648 FAVE Sección Ciencias Veterinarias; Vol. 16 Núm. 1 (2017): FAVE Sección Ciencias Veterinarias; 30-38 FAVE Sección Ciencias Veterinarias; Vol. 16 No. 1 (2017): FAVE Sección Ciencias Veterinarias; 30-38 FAVE Sección Ciencias Veterinarias; v. 16 n. 1 (2017): FAVE Sección Ciencias Veterinarias; 30-38 2362-5589 1666-938X 10.14409/favecv.v16i1 spa https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/6648/9794 Derechos de autor 2017 FAVE Sección Ciencias Veterinarias
institution Universidad Nacional del Litoral
institution_str I-26
repository_str R-133
container_title_str Biblioteca Virtual - Publicaciones (UNL)
language Español
format Artículo revista
topic Escherichia coli
persister cells
mutant prevention
Escherichia coli
bacterias persistentes
concentración preventiva de mutantes
spellingShingle Escherichia coli
persister cells
mutant prevention
Escherichia coli
bacterias persistentes
concentración preventiva de mutantes
PATRICELLI, Paula
RAMIREZ, Eliana
PRESA ROSSA, Candela
DELL'ELCE, Antonella
FORMENTINI, Enrique
Effect of persister cells on the efficacy of enrofloxacin and ciprofloxacin against a strain of Escherichia coli
topic_facet Escherichia coli
persister cells
mutant prevention
Escherichia coli
bacterias persistentes
concentración preventiva de mutantes
author PATRICELLI, Paula
RAMIREZ, Eliana
PRESA ROSSA, Candela
DELL'ELCE, Antonella
FORMENTINI, Enrique
author_facet PATRICELLI, Paula
RAMIREZ, Eliana
PRESA ROSSA, Candela
DELL'ELCE, Antonella
FORMENTINI, Enrique
author_sort PATRICELLI, Paula
title Effect of persister cells on the efficacy of enrofloxacin and ciprofloxacin against a strain of Escherichia coli
title_short Effect of persister cells on the efficacy of enrofloxacin and ciprofloxacin against a strain of Escherichia coli
title_full Effect of persister cells on the efficacy of enrofloxacin and ciprofloxacin against a strain of Escherichia coli
title_fullStr Effect of persister cells on the efficacy of enrofloxacin and ciprofloxacin against a strain of Escherichia coli
title_full_unstemmed Effect of persister cells on the efficacy of enrofloxacin and ciprofloxacin against a strain of Escherichia coli
title_sort effect of persister cells on the efficacy of enrofloxacin and ciprofloxacin against a strain of escherichia coli
description This work evaluated the effect of persister cells present in a high inocula size of a wild strain of Escherichia coli on the efficacy of enrofloxacin and ciprofloxacin by in vitro time-kill curve assays and mutant prevention concentration testing. In time-kill curves performed with high inocula size, no antibiotics showed bactericidal activity, but only allowed the survival of persister cells. In the assay to determine the mutant prevention concentration, on the surface of agar plates containing high enrofloxacin and ciprofloxacin concentrations, persister cells remained viable and without bacterial colonies development and adopting filamentous morphology as a form of adaptation and survival. The clinical usefulness of mutant prevention concentrations of enrofloxacin and ciproflocxacin against Escherichia coli is discussed, as these high concentrations would allow the survival of a sub-population of persister cells originating a biological reservoir that could give rise to chronic infections and favor the emergence of resistant mutants.
publisher Universidad Nacional del Litoral
publishDate 2017
url https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/6648
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first_indexed 2023-07-05T23:06:53Z
last_indexed 2024-08-13T20:38:25Z
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