Genotypic discrimination of serovars belonging to the serogroup Icterohaemorrhagiae of Leptospira interrogans (Spirochaetales: Leptospiraceae) and the analysis of genetic distances using principal coordinates

Leptospirosis is the world`s most widely distributed zoonosis and of great importance in animal health and in public health.  The relevance of the serogroup Icterohaemorrhagiae relies on the frequent isolation from rodents and from clinical cases of humans, canines, bovines and swine; and t...

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Detalles Bibliográficos
Autores principales: GRUNE LOFFLER, Sylvia, MARTINEZ, Mara L, ROMERO, Graciela, BRIHUEGA, Bibiana F.
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional del Litoral 2017
Materias:
Leptospira interrogans
Leptospirosis
Ichterohaemorrhagiae
molecular characterization
Icterohaemorrhagiae
caracterización molecular
Acceso en línea:https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/6243
Aporte de:
Biblioteca Virtual - Publicaciones (UNL) de Universidad Nacional del Litoral
id I26-R133-article-6243
record_format ojs
institution Universidad Nacional del Litoral
institution_str I-26
repository_str R-133
container_title_str Biblioteca Virtual - Publicaciones (UNL)
language Español
format Artículo revista
topic Leptospira interrogans
Leptospirosis
Ichterohaemorrhagiae
molecular characterization
Leptospira interrogans
Leptospirosis
Icterohaemorrhagiae
caracterización molecular
spellingShingle Leptospira interrogans
Leptospirosis
Ichterohaemorrhagiae
molecular characterization
Leptospira interrogans
Leptospirosis
Icterohaemorrhagiae
caracterización molecular
GRUNE LOFFLER, Sylvia
MARTINEZ, Mara L
ROMERO, Graciela
BRIHUEGA, Bibiana F.
Genotypic discrimination of serovars belonging to the serogroup Icterohaemorrhagiae of Leptospira interrogans (Spirochaetales: Leptospiraceae) and the analysis of genetic distances using principal coordinates
topic_facet Leptospira interrogans
Leptospirosis
Ichterohaemorrhagiae
molecular characterization
Leptospira interrogans
Leptospirosis
Icterohaemorrhagiae
caracterización molecular
author GRUNE LOFFLER, Sylvia
MARTINEZ, Mara L
ROMERO, Graciela
BRIHUEGA, Bibiana F.
author_facet GRUNE LOFFLER, Sylvia
MARTINEZ, Mara L
ROMERO, Graciela
BRIHUEGA, Bibiana F.
author_sort GRUNE LOFFLER, Sylvia
title Genotypic discrimination of serovars belonging to the serogroup Icterohaemorrhagiae of Leptospira interrogans (Spirochaetales: Leptospiraceae) and the analysis of genetic distances using principal coordinates
title_short Genotypic discrimination of serovars belonging to the serogroup Icterohaemorrhagiae of Leptospira interrogans (Spirochaetales: Leptospiraceae) and the analysis of genetic distances using principal coordinates
title_full Genotypic discrimination of serovars belonging to the serogroup Icterohaemorrhagiae of Leptospira interrogans (Spirochaetales: Leptospiraceae) and the analysis of genetic distances using principal coordinates
title_fullStr Genotypic discrimination of serovars belonging to the serogroup Icterohaemorrhagiae of Leptospira interrogans (Spirochaetales: Leptospiraceae) and the analysis of genetic distances using principal coordinates
title_full_unstemmed Genotypic discrimination of serovars belonging to the serogroup Icterohaemorrhagiae of Leptospira interrogans (Spirochaetales: Leptospiraceae) and the analysis of genetic distances using principal coordinates
title_sort genotypic discrimination of serovars belonging to the serogroup icterohaemorrhagiae of leptospira interrogans (spirochaetales: leptospiraceae) and the analysis of genetic distances using principal coordinates
description Leptospirosis is the world`s most widely distributed zoonosis and of great importance in animal health and in public health.  The relevance of the serogroup Icterohaemorrhagiae relies on the frequent isolation from rodents and from clinical cases of humans, canines, bovines and swine; and this serogroup was also isolated from water samples of the Reconquista river, Buenos Aires province, Argentina. The objective of this study was to discriminate molecularly between serovars Copenhageni and Icterohaemorrhagiae being of importance in Argentina, using Multiple Locus Variable number tandem repeats analysis (MLVA) and analyzing the genetic distances among genotypes obtained from animal isolates in this study. We genotyped the four referential strains of the serogroup Icterohaemorrhagiae belonging to the species Leptospira interrogans serovar Copenhageni strain M20 and strain Ictero I  and of the serovar Copenhageni strain RGA and strain Fiocruz L1-130. In this study we included 24 strains isolated from animals domestic and wildlife. Using this technique we could determine numeric codes for each serovar and in this way, discriminate between serovars of this serogroup. The principal coordinates analysis (PCoA) showed the genetic variability of the four referential strains from the serogroup Icterohaemorrhagiae, the genotype with better representation was serovar Copenhageni strain Fiocruz L1-130 with 13 isolated strains with this genetic profile.  Discrimination of the serovars from this important serogroup allows to establish differences between clinical signs and/or response to treatment in the clinical cases produced by different serovars of the serogroup Icterohaemorrhagiae successfully distinguished in this study.
publisher Universidad Nacional del Litoral
publishDate 2017
url https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/6243
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spelling I26-R133-article-62432023-09-15T10:28:02Z Genotypic discrimination of serovars belonging to the serogroup Icterohaemorrhagiae of Leptospira interrogans (Spirochaetales: Leptospiraceae) and the analysis of genetic distances using principal coordinates Discriminación genotípica de serovariedades del serogrupo Icterohaemorrhagiae pertenecientes a Leptospira interrogans (Spirochaetales: Leptospiraceae) y el análisis de distancias génicas mediante coordenadas principales GRUNE LOFFLER, Sylvia MARTINEZ, Mara L ROMERO, Graciela BRIHUEGA, Bibiana F. Leptospira interrogans Leptospirosis Ichterohaemorrhagiae molecular characterization Leptospira interrogans Leptospirosis Icterohaemorrhagiae caracterización molecular Leptospirosis is the world`s most widely distributed zoonosis and of great importance in animal health and in public health.  The relevance of the serogroup Icterohaemorrhagiae relies on the frequent isolation from rodents and from clinical cases of humans, canines, bovines and swine; and this serogroup was also isolated from water samples of the Reconquista river, Buenos Aires province, Argentina. The objective of this study was to discriminate molecularly between serovars Copenhageni and Icterohaemorrhagiae being of importance in Argentina, using Multiple Locus Variable number tandem repeats analysis (MLVA) and analyzing the genetic distances among genotypes obtained from animal isolates in this study. We genotyped the four referential strains of the serogroup Icterohaemorrhagiae belonging to the species Leptospira interrogans serovar Copenhageni strain M20 and strain Ictero I  and of the serovar Copenhageni strain RGA and strain Fiocruz L1-130. In this study we included 24 strains isolated from animals domestic and wildlife. Using this technique we could determine numeric codes for each serovar and in this way, discriminate between serovars of this serogroup. The principal coordinates analysis (PCoA) showed the genetic variability of the four referential strains from the serogroup Icterohaemorrhagiae, the genotype with better representation was serovar Copenhageni strain Fiocruz L1-130 with 13 isolated strains with this genetic profile.  Discrimination of the serovars from this important serogroup allows to establish differences between clinical signs and/or response to treatment in the clinical cases produced by different serovars of the serogroup Icterohaemorrhagiae successfully distinguished in this study. La leptospirosis es una zoonosis de amplia distribución mundial y de importancia en salud pública y veterinaria. El serogrupo Icterohaemorrhagiae toma importancia, ya que ha sido aislado con frecuencia en roedores y en casos clínicos de humanos, caninos, bovinos y porcinos; y se ha logrado aislar a partir de agua del rio Reconquista, provincia de Buenos Aires, Argentina.  El objetivo de este estudio fue discriminar molecularmente entre las serovariedades Copenhageni e Icterohaemorrhagiae del serogrupo Icterohaemorrhagiae de importancia en Argentina,  utilizando  la técnica del análisis de repeticiones en tándem en múltiples locus (MLVA) y analizar las distancias génicas entre los genotipos determinados a partir de cepas aisladas de animales. Para ello, geno-tipificamos las 4 cepas referenciales del serogrupo Icterohaemorrhagiae de la especie Leptospira interrogans serovariedad Copenhageni cepa M20 y cepa Ictero I y  de la serovariedad Copenhageni cepa RGA y cepa Fiocruz L1-130. Para este estudio se incluyeron 24 cepas aisladas de animales domésticos como silvestres. Logramos mediante esta técnica determinar un código numérico para cada serovariedad y así pudimos discriminar entre las  serovariedades de este serogrupo. El análisis de coordenadas principales (PCoA) demostró la variabilidad génica existente entre las 4 cepas referenciales pertenecientes al serogrupo Icterohaemorrhagiae, el genotipo con mayor representación fue el serovar Copenhageni cepa Fiocruz L1-130 con un total de 13 cepas aisladas con ese perfil genético. Discriminar las serovariedades de este importante serogrupo nos permitirá en un futuro investigar si existen diferencias entre la sintomatología clínica y/o respuesta al tratamiento en los casos clínicos producidos por las distintas serovariedades del serogrupo Icterohaemorrhagiae discriminadas en este trabajo. Universidad Nacional del Litoral 2017-01-18 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artículo original Original article application/pdf https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/6243 10.14409/favecv.v15i1/2.6243 FAVE Sección Ciencias Veterinarias; Vol. 15 Núm. 1/2 (2016): FAVE Sección Ciencias Veterinarias; 31-37 FAVE Sección Ciencias Veterinarias; Vol. 15 No. 1/2 (2016): FAVE Sección Ciencias Veterinarias; 31-37 FAVE Sección Ciencias Veterinarias; v. 15 n. 1/2 (2016): FAVE Sección Ciencias Veterinarias; 31-37 2362-5589 1666-938X 10.14409/favecv.v15i1/2 spa https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/6243/9221 Derechos de autor 2017 FAVE Sección Ciencias Veterinarias

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