Evaluación de la capacidad antagónica de cepas nativas de Trichoderma spp. frente a Verticillium dahliae en la provincia de Catamarca
The antagonistic capacity of native strains of Trichoderma spp. against Verticillium dahliae, under different culture and incubation conditions. The isolation and molecular identification of antagonistic native strains and the phytopathogen were carried out. They were evaluated in two in vitro tests...
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| Lenguaje: | Español |
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Universidad Nacional del Litoral
2021
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| Acceso en línea: | https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEAgrarias/article/view/10623 |
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I26-R133-article-106232024-06-20T01:13:17Z Evaluación de la capacidad antagónica de cepas nativas de Trichoderma spp. frente a Verticillium dahliae en la provincia de Catamarca Evaluation of the antagonic capacity of native strains of Trichoderma spp. in front of Verticillium dahliae in the province of Catamarca. González Basso, M. V. Picón, M. C. Ribero, G. Bellone, C. biocontrol parasitism grape marc photoperiod pH biocontrol parasitismo orujo de vid fotoperiodo pH The antagonistic capacity of native strains of Trichoderma spp. against Verticillium dahliae, under different culture and incubation conditions. The isolation and molecular identification of antagonistic native strains and the phytopathogen were carried out. They were evaluated in two in vitro tests, using ADP culture medium in two pH conditions and incubated under two lighting conditions. By means of dual culture techniques, a trial was carried out with a completely randomized design with a 2 x 2 x 2 factorial arrangement, and microcultures. The antagonistic capacity of two native strains of Trichoderma, TT (T. hamatum) and M5A (T. hamatum) on the native phytopathogen strain (Verticillium dahliae) was evaluated. The results obtained showed that the TT strain at pH 6.5 with 16 h light / 8 h Darkness had the shortest Contact Time and the highest Growth Inhibition Percentage with 8 h of light /16 h. of darkness. Class 1 was obtained in both tests and treatments, both being efficient in the competition for substrate and space. It was observed that the native strain M5A and the standard strain TT, parasitize the phytopathogen. Therefore, it is concluded that the TT strain has greater antagonistic attributes against the phytopathogen V. dahliae, under conditions of 8 h light / 16 h dark. Se evaluó la capacidad antagónica de cepas nativas de Trichoderma spp. frente a Verticillium dahliae en diferentes condiciones de cultivo e incubación. Se realizó el aislamiento e identificación molecular de cepas nativas antagónicas y del fitopatógeno. Los mismos fueron evaluadas en dos ensayos in vitro, empleando medio de cultivo ADP en dos condiciones de pH e incubados bajo dos condiciones de iluminación. Mediante técnicas de cultivo dual se efectuó un ensayo con diseño completamente aleatorizado con arreglo factorial 2 x 2 x 2, y de microcultivos. Se evaluó la capacidad antagonista de dos cepas nativas de Trichoderma, TT (T. hamatum) y M5A (T. hamatum) sobre la cepa del fitopatógeno nativo (Verticillium dahliae). Los resultados obtenidos demostraron que la cepa TT a pH 6.5 con 16 h luz/8 h oscuridad tuvo el menor Tiempo de Contacto y mayor Porcentaje de Inhibición de Crecimiento con 8 h de luz/16 h. de oscuridad. En ambos ensayos y tratamientos se obtuvo Clase 1, siendo ambas eficientes en la competencia por sustrato y espacio. Se observó que la cepa nativa M5A y la cepa patrón TT parasitan al fitopatógeno. Por lo que se concluye que la cepa TT presenta mayores atributos de antagonismo frente al fitopatógeno V. dahliae, en condiciones de 8 h luz/ 16 h oscuridad. Universidad Nacional del Litoral 2021-09-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEAgrarias/article/view/10623 10.14409/fa.v20i2.10623 FAVE Sección Ciencias Agrarias; Vol. 20 Núm. 2 (2021): FAVE Sección Ciencias Agrarias; 33-44 FAVE Sección Ciencias Agrarias; Vol. 20 No. 2 (2021): FAVE Sección Ciencias Agrarias; 33-44 FAVE Sección Ciencias Agrarias; v. 20 n. 2 (2021): FAVE Sección Ciencias Agrarias; 33-44 2346-9129 1666-7719 10.14409/fa.v20i2 spa https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEAgrarias/article/view/10623/14124 https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEAgrarias/article/view/10623/14125 Derechos de autor 2021 FAVE Sección Ciencias Agrarias |
| institution |
Universidad Nacional del Litoral |
| institution_str |
I-26 |
| repository_str |
R-133 |
| container_title_str |
Biblioteca Virtual - Publicaciones (UNL) |
| language |
Español |
| format |
Artículo revista |
| topic |
biocontrol parasitism grape marc photoperiod pH biocontrol parasitismo orujo de vid fotoperiodo pH |
| spellingShingle |
biocontrol parasitism grape marc photoperiod pH biocontrol parasitismo orujo de vid fotoperiodo pH González Basso, M. V. Picón, M. C. Ribero, G. Bellone, C. Evaluación de la capacidad antagónica de cepas nativas de Trichoderma spp. frente a Verticillium dahliae en la provincia de Catamarca |
| topic_facet |
biocontrol parasitism grape marc photoperiod pH biocontrol parasitismo orujo de vid fotoperiodo pH |
| author |
González Basso, M. V. Picón, M. C. Ribero, G. Bellone, C. |
| author_facet |
González Basso, M. V. Picón, M. C. Ribero, G. Bellone, C. |
| author_sort |
González Basso, M. V. |
| title |
Evaluación de la capacidad antagónica de cepas nativas de Trichoderma spp. frente a Verticillium dahliae en la provincia de Catamarca |
| title_short |
Evaluación de la capacidad antagónica de cepas nativas de Trichoderma spp. frente a Verticillium dahliae en la provincia de Catamarca |
| title_full |
Evaluación de la capacidad antagónica de cepas nativas de Trichoderma spp. frente a Verticillium dahliae en la provincia de Catamarca |
| title_fullStr |
Evaluación de la capacidad antagónica de cepas nativas de Trichoderma spp. frente a Verticillium dahliae en la provincia de Catamarca |
| title_full_unstemmed |
Evaluación de la capacidad antagónica de cepas nativas de Trichoderma spp. frente a Verticillium dahliae en la provincia de Catamarca |
| title_sort |
evaluación de la capacidad antagónica de cepas nativas de trichoderma spp. frente a verticillium dahliae en la provincia de catamarca |
| description |
The antagonistic capacity of native strains of Trichoderma spp. against Verticillium dahliae, under different culture and incubation conditions. The isolation and molecular identification of antagonistic native strains and the phytopathogen were carried out. They were evaluated in two in vitro tests, using ADP culture medium in two pH conditions and incubated under two lighting conditions. By means of dual culture techniques, a trial was carried out with a completely randomized design with a 2 x 2 x 2 factorial arrangement, and microcultures. The antagonistic capacity of two native strains of Trichoderma, TT (T. hamatum) and M5A (T. hamatum) on the native phytopathogen strain (Verticillium dahliae) was evaluated. The results obtained showed that the TT strain at pH 6.5 with 16 h light / 8 h Darkness had the shortest Contact Time and the highest Growth Inhibition Percentage with 8 h of light /16 h. of darkness. Class 1 was obtained in both tests and treatments, both being efficient in the competition for substrate and space. It was observed that the native strain M5A and the standard strain TT, parasitize the phytopathogen. Therefore, it is concluded that the TT strain has greater antagonistic attributes against the phytopathogen V. dahliae, under conditions of 8 h light / 16 h dark. |
| publisher |
Universidad Nacional del Litoral |
| publishDate |
2021 |
| url |
https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEAgrarias/article/view/10623 |
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2023-07-05T22:51:49Z |
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2024-08-13T20:36:22Z |
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