Comparative study on the pharmacokinetics of Ginkgo biloba extract between normal and diabetic rats by HPLC-DAD
A new HPLC-DAD method has been developed and validated for the simultaneous analysis of five flavonoids (rutin, quercitrin, quercetin, kaempferol, and isorahamnetin) in rat plasma. Sample pretreatment consisted of a liquid–liquid extraction. The five flavonoids was separated on a kromasil C18 column...
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| Autores principales: | , , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2009
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/7775 http://www.latamjpharm.org/trabajos/28/3/LAJOP_28_3_1_11_N804613NI3.pdf |
| Aporte de: |
| Sumario: | A new HPLC-DAD method has been developed and validated for the simultaneous analysis of five flavonoids (rutin, quercitrin, quercetin, kaempferol, and isorahamnetin) in rat plasma. Sample pretreatment consisted of a liquid–liquid extraction. The five flavonoids was separated on a kromasil C18 column and recorded at 350 nm. The greatest resolution was achieved with methanol-0.1% formic acid gradient at a flow rate of 1.0 mL min<sup>-1</sup> (The correlation coefficients for all the calibration plots (r > 0.999 . showed good linearity over the range tested. The relative standard deviation of the method was less than 7% and 10% for intra and inter-day assays respectively, and average recovery was between 77.2 and 99.2%. The detection limits of this method were between 0.006 and 0.02 μg mL<sup>-1</sup> The method has been . successfully applied to determine drug concentrations in normal and diabetic rat plasma samples and pharmacokinetic of Ginkgo biloba extract |
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