An Enzymatic–Colorimetric Assay for the Quantification of <i>Bifidobacterium</i>
An enzymatic-colorimetric assay for the quantification of <i>Bifidobacterium</i> was developed. The method, based upon the standard detection of fructose-6-phosphate phosphoketolase activity, was optimized with respect to bacterial cell pretreatment, time of incubation, and substrate con...
Guardado en:
| Autores principales: | , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2000
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/141899 |
| Aporte de: |
| Sumario: | An enzymatic-colorimetric assay for the quantification of <i>Bifidobacterium</i> was developed. The method, based upon the standard detection of fructose-6-phosphate phosphoketolase activity, was optimized with respect to bacterial cell pretreatment, time of incubation, and substrate concentration. The relationship between bacterial biomass and phosphoketolase activity was linear in a wide spectrum of bacterial densities. Higher sensitivity over the standard method was achieved by using 0.25% Triton X-100 in the reaction mixture to pretreat the bacterial cells. Because autoaggregation is a frequent feature among <i>Bifidobacterium</i> strains, this simple and reproducible method offers good advantage over viable plate count and turbidimetric techniques. The methodology can also be applied to the assessment of adherent <i>Bifidobacterium</i> strains to human epithelial cells. |
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