Tomato wall-associated kinase SlWak1 acts in an Fls2- and Fls3-dependent manner to promote apoplastic immune responses to <i>Pseudomonas syringae</i>
Wall-associated kinases (Waks) are known to be important components of plant immunity against various pathogens including <i>Pseudomonas syringae</i> pv. tomato (Pst) although their molecular mechanisms are largely unknown. In tomato, <i>SlWak1</i> has been implicated in immu...
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| Autores principales: | , , , |
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| Formato: | Articulo Preprint |
| Lenguaje: | Inglés |
| Publicado: |
2020
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/125269 |
| Aporte de: |
| Sumario: | Wall-associated kinases (Waks) are known to be important components of plant immunity against various pathogens including <i>Pseudomonas syringae</i> pv. tomato (Pst) although their molecular mechanisms are largely unknown. In tomato, <i>SlWak1</i> has been implicated in immunity because its transcript abundance increases significantly in leaves after treatment with the flagellin-derived peptides flg22 and flgII-28, which activate the receptors Fls2 and Fls3, respectively. We generated two <i>SlWak1</i> tomato mutants (Δwak1) using CRISPR/Cas9 and investigated the role of <i>SlWak1</i> in tomato-Pst interactions. PTI activated in the apoplast by flg22 or flgII-28 was compromised in Δwak1 plants but PTI at the leaf surface was unaffected. The Δwak1 plants developed fewer callose deposits than wild-type plants but retained the ability to generate reactive oxygen species and activate MAPKs in response to flg22 and flgII-28. The induction of <i>Wak1</i> gene expression by flg22 and flgII-28 was greatly reduced in a tomato mutant lacking Fls2 and Fls3 but induction of <i>Fls3</i> gene expression by flgII-28 was unaffected in Δwak1 plants. After <i>Pst</i> inoculation, Δwak1 plants developed disease symptoms more slowly than Δfls2.1/fls2.2/fls3 mutant plants, although both plants ultimately were similarly susceptible. SlWak1 co-immunoprecipitated with both Fls2 and Fls3 independently of flg22/flgII-28 or Bak1. These observations suggest that SlWak1 acts in a complex with Fls2/Fls3 and plays an important role at later stages of the PTI in the apoplast. |
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