Overexpression of the carbohydrate binding module from <i>Solanum lycopersicum</i> expansin 1 (Sl-EXP1) modifies tomato fruit firmness and <i>Botrytis cinerea</i> susceptibility

Firmness, one of the major determinants of postharvest quality and shelf life of fruits is determined by the mechanical resistance imposed by the plant cell wall. Expansins (EXP) are involved in the nonhydrolytic metabolic disassembly of plant cell walls, particularly in processes where relaxation o...

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Autores principales: Perini, Mauro Alejandro, Sin, Ignacio Nicolás, Villarreal, N. M., Marina, M., Powell, A. L. T., Martínez, Gustavo Adolfo, Civello, Pedro Marcos
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Agrarias
CBM
Fruit softening
Cell wall
Ripening
Postharvest
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/118154
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-118154
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Agrarias
CBM
Fruit softening
Cell wall
Ripening
Postharvest
spellingShingle Ciencias Agrarias
CBM
Fruit softening
Cell wall
Ripening
Postharvest
Perini, Mauro Alejandro
Sin, Ignacio Nicolás
Villarreal, N. M.
Marina, M.
Powell, A. L. T.
Martínez, Gustavo Adolfo
Civello, Pedro Marcos
Overexpression of the carbohydrate binding module from <i>Solanum lycopersicum</i> expansin 1 (Sl-EXP1) modifies tomato fruit firmness and <i>Botrytis cinerea</i> susceptibility
topic_facet Ciencias Agrarias
CBM
Fruit softening
Cell wall
Ripening
Postharvest
description Firmness, one of the major determinants of postharvest quality and shelf life of fruits is determined by the mechanical resistance imposed by the plant cell wall. Expansins (EXP) are involved in the nonhydrolytic metabolic disassembly of plant cell walls, particularly in processes where relaxation of the wall is necessary, such as fruit development and ripening. As many carbohydrate-associated proteins, expansins have a putative catalytic domain and a carbohydrate-binding module (CBM). Several strategies have been pursued to control the loss of fruit firmness during storage. Most of the approaches have been to suppress the expression of key enzymes involved in the cell wall metabolism, but this is the first time that a CBM was overexpressed in a fruit aimed to control cell wall degradation and fruit softening. We report the constitutive overexpression of the CBM of Solanum lycopersicum expansin 1 (CBMSlExp1) in the cell wall of tomato plants, and its effects on plant and fruit phenotype. Overexpression of CBM-SlExp1 increased the mechanical resistance of leaves, whereas it did not modify plant growth and general phenotype. However, transgenic plants showed delayed softening and firmer fruits. In addition, fruits were less susceptible to Botrytis cinerea infection, and the “in vitro” growth of the fungus on media containing AIR from the pericarp of transgenic fruits was lower than controls. The possibility of overexpressing a CBM of a fruit-specific expansin to control cell wall degradation and fruit softening is discussed.
format Articulo
Articulo
author Perini, Mauro Alejandro
Sin, Ignacio Nicolás
Villarreal, N. M.
Marina, M.
Powell, A. L. T.
Martínez, Gustavo Adolfo
Civello, Pedro Marcos
author_facet Perini, Mauro Alejandro
Sin, Ignacio Nicolás
Villarreal, N. M.
Marina, M.
Powell, A. L. T.
Martínez, Gustavo Adolfo
Civello, Pedro Marcos
author_sort Perini, Mauro Alejandro
title Overexpression of the carbohydrate binding module from <i>Solanum lycopersicum</i> expansin 1 (Sl-EXP1) modifies tomato fruit firmness and <i>Botrytis cinerea</i> susceptibility
title_short Overexpression of the carbohydrate binding module from <i>Solanum lycopersicum</i> expansin 1 (Sl-EXP1) modifies tomato fruit firmness and <i>Botrytis cinerea</i> susceptibility
title_full Overexpression of the carbohydrate binding module from <i>Solanum lycopersicum</i> expansin 1 (Sl-EXP1) modifies tomato fruit firmness and <i>Botrytis cinerea</i> susceptibility
title_fullStr Overexpression of the carbohydrate binding module from <i>Solanum lycopersicum</i> expansin 1 (Sl-EXP1) modifies tomato fruit firmness and <i>Botrytis cinerea</i> susceptibility
title_full_unstemmed Overexpression of the carbohydrate binding module from <i>Solanum lycopersicum</i> expansin 1 (Sl-EXP1) modifies tomato fruit firmness and <i>Botrytis cinerea</i> susceptibility
title_sort overexpression of the carbohydrate binding module from <i>solanum lycopersicum</i> expansin 1 (sl-exp1) modifies tomato fruit firmness and <i>botrytis cinerea</i> susceptibility
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/118154
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