Protein kinase C activity in boar sperm

Male germ cells undergo different processes within the female reproductive tract to successfully fertilize the oocyte. These processes are triggered by different extracellular stimuli leading to activation of protein phosphorylation. Protein kinase C (PKC) is a key regulatory enzyme in signal tran...

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Detalles Bibliográficos
Autores principales: Teijeiro, Juan Manuel, Marini, Patricia Estela, Bragado, María Julia, García-Marin, Luis
Formato: article artículo acceptedVersion
Lenguaje:Inglés
Publicado: Wiley 2017
Materias:
Espermatozoides porcinos
Fluidez de membrana
Protein quinasa C
Boar sperm
Membrane fluidity
Protein kinase C
Acceso en línea:http://hdl.handle.net/2133/9294
http://hdl.handle.net/2133/9294
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-9294
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Espermatozoides porcinos
Fluidez de membrana
Protein quinasa C
Boar sperm
Membrane fluidity
Protein kinase C
spellingShingle Espermatozoides porcinos
Fluidez de membrana
Protein quinasa C
Boar sperm
Membrane fluidity
Protein kinase C
Teijeiro, Juan Manuel
Marini, Patricia Estela
Bragado, María Julia
García-Marin, Luis
Protein kinase C activity in boar sperm
topic_facet Espermatozoides porcinos
Fluidez de membrana
Protein quinasa C
Boar sperm
Membrane fluidity
Protein kinase C
description Male germ cells undergo different processes within the female reproductive tract to successfully fertilize the oocyte. These processes are triggered by different extracellular stimuli leading to activation of protein phosphorylation. Protein kinase C (PKC) is a key regulatory enzyme in signal transduction mechanisms involved in many cellular processes. Studies in boar sperm demonstrated a role for PKC in the intracellular signaling involved in motility and cellular volume regulation. Experiments using phorbol 12-myristate 13-acetate (PMA) showed increases in the Serine/Threonine phosphorylation of substrates downstream of PKC in boar sperm. In order to gain knowledge about those cellular processes regulated by PKC, we evaluate the effects of PMA on boar sperm motility, lipid organization of plasma membrane, integrity of acrosome membrane and sperm agglutination. Also, we investigate the crosstalk between PKA and PKC intracellular pathways in spermatozoa from this species. The results presented here reveal a participation of PKC in sperm motility regulation and membrane fluidity changes, which is probably associated to acrosome reaction and to agglutination. Also, we show the existence of a hierarchy in the kinases pathway. Previous works on boar sperm suggest a pathway in which PKA is positioned upstream to PKC and this new results support such model.
format article
artículo
acceptedVersion
author Teijeiro, Juan Manuel
Marini, Patricia Estela
Bragado, María Julia
García-Marin, Luis
author_facet Teijeiro, Juan Manuel
Marini, Patricia Estela
Bragado, María Julia
García-Marin, Luis
author_sort Teijeiro, Juan Manuel
title Protein kinase C activity in boar sperm
title_short Protein kinase C activity in boar sperm
title_full Protein kinase C activity in boar sperm
title_fullStr Protein kinase C activity in boar sperm
title_full_unstemmed Protein kinase C activity in boar sperm
title_sort protein kinase c activity in boar sperm
publisher Wiley
publishDate 2017
url http://hdl.handle.net/2133/9294
http://hdl.handle.net/2133/9294
work_keys_str_mv AT teijeirojuanmanuel proteinkinasecactivityinboarsperm
AT marinipatriciaestela proteinkinasecactivityinboarsperm
AT bragadomariajulia proteinkinasecactivityinboarsperm
AT garciamarinluis proteinkinasecactivityinboarsperm
bdutipo_str Repositorios
_version_ 1764820413845602305

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