Trypanosoma cruzi Experimental Infection Impacts on the Thymic Regulatory T Cell Compartment

The dynamics of regulatory T cells in the course of Trypanosoma cruzi infection is still debated. We previously demonstrated that acute murine T. cruzi infection results in an impaired peripheral CD4+Foxp3+ T cell differentiation due to the acquisition of an abnormal Th1-like phenotype and altered f...

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Detalles Bibliográficos
Autores principales: González, Florencia Belén, Calmon-Hamaty, Flavia, Cordeiro, Synara Nô Seara, Fernández Bussy, Rodrigo, Spinelli, Silvana Virginia, D'Attilio, Luciano, Bottasso, Oscar, Savino, Wilson, Cotta-de-Almeida, Vinícius, Raquel Villar, Silvina, Pérez, Ana Rosa
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: PLoS 2017
Materias:
Trypanosoma cruzi
Thymocytes
Thymus
Acceso en línea:http://hdl.handle.net/2133/7385
http://hdl.handle.net/2133/7385
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-7385
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Trypanosoma cruzi
Thymocytes
Thymus
spellingShingle Trypanosoma cruzi
Thymocytes
Thymus
González, Florencia Belén
Calmon-Hamaty, Flavia
Cordeiro, Synara Nô Seara
Fernández Bussy, Rodrigo
Spinelli, Silvana Virginia
D'Attilio, Luciano
Bottasso, Oscar
Savino, Wilson
Cotta-de-Almeida, Vinícius
Raquel Villar, Silvina
Pérez, Ana Rosa
Trypanosoma cruzi Experimental Infection Impacts on the Thymic Regulatory T Cell Compartment
topic_facet Trypanosoma cruzi
Thymocytes
Thymus
description The dynamics of regulatory T cells in the course of Trypanosoma cruzi infection is still debated. We previously demonstrated that acute murine T. cruzi infection results in an impaired peripheral CD4+Foxp3+ T cell differentiation due to the acquisition of an abnormal Th1-like phenotype and altered functional features, negatively impacting on the course of infection. Moreover, T. cruzi infection induces an intense thymic atrophy. As known, the thymus is the primary lymphoid organ in which thymic-derived regulatory T cells, known as tTregs, differentiate. Considering the lack of available data about the effect of T. cruzi infection upon tTregs, we examined tTreg dynamics during the course of disease. We confirmed that T. cruzi infection induces a marked loss of tTreg cell number associated to cell precursor exhaustion, partially avoided by glucocorticoid ablation- and IL-2 survival factor depletion. At the same time, tTregs accumulate within the CD4 single-positive compartment, exhibiting an increased Ki-67/Annexin V ratio compared to controls. Moreover, tTregs enhance after the infection the expression of signature markers (CD25, CD62L and GITR) and they also display alterations in the expression of migration-associated molecules (α chains of VLAs and chemokine receptors) such as functional fibronectin-driven migratory disturbance. Taken together, we provide data demonstrating profound alterations in tTreg compartment during acute murine T. cruzi infection, denoting that their homeostasis is significantly affected. The evident loss of tTreg cell number may compromise the composition of tTreg peripheral pool, and such sustained alteration over time may be partially related to the immune dysregulation observed in the chronic phase of the disease.
format article
artículo
publishedVersion
author González, Florencia Belén
Calmon-Hamaty, Flavia
Cordeiro, Synara Nô Seara
Fernández Bussy, Rodrigo
Spinelli, Silvana Virginia
D'Attilio, Luciano
Bottasso, Oscar
Savino, Wilson
Cotta-de-Almeida, Vinícius
Raquel Villar, Silvina
Pérez, Ana Rosa
author_facet González, Florencia Belén
Calmon-Hamaty, Flavia
Cordeiro, Synara Nô Seara
Fernández Bussy, Rodrigo
Spinelli, Silvana Virginia
D'Attilio, Luciano
Bottasso, Oscar
Savino, Wilson
Cotta-de-Almeida, Vinícius
Raquel Villar, Silvina
Pérez, Ana Rosa
author_sort González, Florencia Belén
title Trypanosoma cruzi Experimental Infection Impacts on the Thymic Regulatory T Cell Compartment
title_short Trypanosoma cruzi Experimental Infection Impacts on the Thymic Regulatory T Cell Compartment
title_full Trypanosoma cruzi Experimental Infection Impacts on the Thymic Regulatory T Cell Compartment
title_fullStr Trypanosoma cruzi Experimental Infection Impacts on the Thymic Regulatory T Cell Compartment
title_full_unstemmed Trypanosoma cruzi Experimental Infection Impacts on the Thymic Regulatory T Cell Compartment
title_sort trypanosoma cruzi experimental infection impacts on the thymic regulatory t cell compartment
publisher PLoS
publishDate 2017
url http://hdl.handle.net/2133/7385
http://hdl.handle.net/2133/7385
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