Trypanosoma cruzi Infection through the Oral Route Promotes a Severe Infection in Mice: New Disease Form from an Old Infection?

Oral transmission of Chagas disease has been documented in Latin American countries. Nevertheless, significant studies on the pathophysiology of this form of infection are largely lacking. The few studies investigating oral route infection disregard that inoculation in the oral cavity (Oral infectio...

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Autores principales: Barreto-de-Albuquerque, Juliana, Silva-dos-Santos, Danielle, Pérez, Ana Rosa, Berbert, Luiz Ricardo, de Santana-van-Vliet, Eliane, Farias-de-Oliveira, Désio Aurélio, Moreira, Otacilio C., Roggero, Eduardo, de Carvalho-Pinto, Carla Eponina, Jurberg, José, Cotta-de-Almeida, Vinícius, Bottasso, Oscar, Savino, Wilson, de Meis, Juliana
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: PLOS (Public Library of Science) 2015
Materias:
Genitourinary infections
Parasitic diseases
Trypanosoma cruzi
Gastrointestinal infections
Heart
Trypomastigotes
Parasitemia
Acceso en línea:http://hdl.handle.net/2133/4879
http://hdl.handle.net/2133/4879
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-4879
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Genitourinary infections
Parasitic diseases
Trypanosoma cruzi
Gastrointestinal infections
Heart
Trypomastigotes
Parasitemia
spellingShingle Genitourinary infections
Parasitic diseases
Trypanosoma cruzi
Gastrointestinal infections
Heart
Trypomastigotes
Parasitemia
Barreto-de-Albuquerque, Juliana
Silva-dos-Santos, Danielle
Pérez, Ana Rosa
Berbert, Luiz Ricardo
de Santana-van-Vliet, Eliane
Farias-de-Oliveira, Désio Aurélio
Moreira, Otacilio C.
Roggero, Eduardo
de Carvalho-Pinto, Carla Eponina
Jurberg, José
Cotta-de-Almeida, Vinícius
Bottasso, Oscar
Savino, Wilson
de Meis, Juliana
Trypanosoma cruzi Infection through the Oral Route Promotes a Severe Infection in Mice: New Disease Form from an Old Infection?
topic_facet Genitourinary infections
Parasitic diseases
Trypanosoma cruzi
Gastrointestinal infections
Heart
Trypomastigotes
Parasitemia
description Oral transmission of Chagas disease has been documented in Latin American countries. Nevertheless, significant studies on the pathophysiology of this form of infection are largely lacking. The few studies investigating oral route infection disregard that inoculation in the oral cavity (Oral infection, OI) or by gavage (Gastrointestinal infection, GI) represent different infection routes, yet both show clear-cut parasitemia and heart parasitism during the acute infection. Herein, BALB/c mice were subjected to acute OI or GI infection using 5x104 culture-derived Trypanosoma cruzi trypomastigotes. OI mice displayed higher parasitemia and mortality rates than their GI counterparts. Heart histopathology showed larger areas of infiltration in the GI mice, whereas liver lesions were more severe in the OI animals, accompanied by higher Alanine Transaminase and Aspartate Transaminase serum contents. A differential cytokine pattern was also observed because OI mice presented higher pro-inflammatory cytokine (IFN-γ, TNF) serum levels than GI animals. Real-time PCR confirmed a higher TNF, IFN-γ, as well as IL-10 expression in the cardiac tissue from the OI group compared with GI. Conversely, TGF-β and IL-17 serum levels were greater in the GI animals. Immunolabeling revealed macrophages as the main tissue source of TNF in infected mice. The high mortality rate observed in the OI mice paralleled the TNF serum rise, with its inhibition by an anti-TNF treatment. Moreover, differences in susceptibility between GI versus OI mice were more clearly related to the host response than to the effect of gastric pH on parasites, since infection in magnesium hydroxide-treated mice showed similar results. Overall, the present study provides conclusive evidence that the initial site of parasite entrance critically affects host immune response and disease outcome. In light of the occurrence of oral Chagas disease outbreaks, our results raise important implications in terms of the current view of the natural disease course and host-parasite relationship.
format article
artículo
publishedVersion
author Barreto-de-Albuquerque, Juliana
Silva-dos-Santos, Danielle
Pérez, Ana Rosa
Berbert, Luiz Ricardo
de Santana-van-Vliet, Eliane
Farias-de-Oliveira, Désio Aurélio
Moreira, Otacilio C.
Roggero, Eduardo
de Carvalho-Pinto, Carla Eponina
Jurberg, José
Cotta-de-Almeida, Vinícius
Bottasso, Oscar
Savino, Wilson
de Meis, Juliana
author_facet Barreto-de-Albuquerque, Juliana
Silva-dos-Santos, Danielle
Pérez, Ana Rosa
Berbert, Luiz Ricardo
de Santana-van-Vliet, Eliane
Farias-de-Oliveira, Désio Aurélio
Moreira, Otacilio C.
Roggero, Eduardo
de Carvalho-Pinto, Carla Eponina
Jurberg, José
Cotta-de-Almeida, Vinícius
Bottasso, Oscar
Savino, Wilson
de Meis, Juliana
author_sort Barreto-de-Albuquerque, Juliana
title Trypanosoma cruzi Infection through the Oral Route Promotes a Severe Infection in Mice: New Disease Form from an Old Infection?
title_short Trypanosoma cruzi Infection through the Oral Route Promotes a Severe Infection in Mice: New Disease Form from an Old Infection?
title_full Trypanosoma cruzi Infection through the Oral Route Promotes a Severe Infection in Mice: New Disease Form from an Old Infection?
title_fullStr Trypanosoma cruzi Infection through the Oral Route Promotes a Severe Infection in Mice: New Disease Form from an Old Infection?
title_full_unstemmed Trypanosoma cruzi Infection through the Oral Route Promotes a Severe Infection in Mice: New Disease Form from an Old Infection?
title_sort trypanosoma cruzi infection through the oral route promotes a severe infection in mice: new disease form from an old infection?
publisher PLOS (Public Library of Science)
publishDate 2015
url http://hdl.handle.net/2133/4879
http://hdl.handle.net/2133/4879
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