Influence of Physicochemical Factors on Adsorption of Ten Shigella flexneri Phages

Bacterial viruses known as bacteriophages have been demonstrated to be effective in killing foodborne pathogens such as Shigella flexneri. Adsorption is the first step in the phage–host interaction. In the present work, 10 Shigella phages were used to characterize the adsorption process on Shigella...

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Detalles Bibliográficos
Autores principales: Tomat, David Damián, Aquili, Virginia, Casabonne, Cecilia, Quiberoni, Andrea
Lenguaje:Inglés
Publicado: MDPI 2023
Materias:
Bacteriophage
Adsorption
Shigella flexneri
Food safety
Acceso en línea:http://hdl.handle.net/2133/25160
http://hdl.handle.net/2133/25160
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-25160
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
topic Bacteriophage
Adsorption
Shigella flexneri
Food safety
spellingShingle Bacteriophage
Adsorption
Shigella flexneri
Food safety
Tomat, David Damián
Aquili, Virginia
Casabonne, Cecilia
Quiberoni, Andrea
Influence of Physicochemical Factors on Adsorption of Ten Shigella flexneri Phages
topic_facet Bacteriophage
Adsorption
Shigella flexneri
Food safety
description Bacterial viruses known as bacteriophages have been demonstrated to be effective in killing foodborne pathogens such as Shigella flexneri. Adsorption is the first step in the phage–host interaction. In the present work, 10 Shigella phages were used to characterize the adsorption process on Shigella flexneri ATCC12022 in several physicochemical conditions related to food and in a food matrix. One-step growth curves were drawn for all the Shigella-phages evaluated. Furthermore, the adsorption rate for each of the 10 phages was determined. In addition, the influence of temperature, Na+, Mg2+, pH, sucrose and glycerol on phage adsorption was investigated. Two phages (Shi22 and Shi30) showed higher burst sizes values (67 and 64 PFU cell−1, respectively) and burst times of 25 min to 30 min, while the other eight phages exhibited burst sizes ranging from 14 to 17 PFU cell−1 with slower burst times. Furthermore, most phages achieved a high adsorption rate, and the adsorption constants (k) ranged from ~10−9 to 10−10 mL min−1. Regarding the influence of temperature, cations and pH, a high or moderate percentage of adsorption was observed for most of the phages evaluated. The adsorption decreased at increasing concentrations of Na+, sucrose and glycerol, although at different levels, since adsorption was more affected by sucrose than by glycerol and Na+ for most phages. The adsorption obtained in Triptein soy broth (TSB) for most of the phages/strain systems evaluated was moderate or high, as well as those observed in a food matrix. Thus, our phages could potentially be used to improve food safety under a wide range of environmental conditions against foodborne pathogens.
author Tomat, David Damián
Aquili, Virginia
Casabonne, Cecilia
Quiberoni, Andrea
author_facet Tomat, David Damián
Aquili, Virginia
Casabonne, Cecilia
Quiberoni, Andrea
author_sort Tomat, David Damián
title Influence of Physicochemical Factors on Adsorption of Ten Shigella flexneri Phages
title_short Influence of Physicochemical Factors on Adsorption of Ten Shigella flexneri Phages
title_full Influence of Physicochemical Factors on Adsorption of Ten Shigella flexneri Phages
title_fullStr Influence of Physicochemical Factors on Adsorption of Ten Shigella flexneri Phages
title_full_unstemmed Influence of Physicochemical Factors on Adsorption of Ten Shigella flexneri Phages
title_sort influence of physicochemical factors on adsorption of ten shigella flexneri phages
publisher MDPI
publishDate 2023
url http://hdl.handle.net/2133/25160
http://hdl.handle.net/2133/25160
work_keys_str_mv AT tomatdaviddamian influenceofphysicochemicalfactorsonadsorptionoftenshigellaflexneriphages
AT aquilivirginia influenceofphysicochemicalfactorsonadsorptionoftenshigellaflexneriphages
AT casabonnececilia influenceofphysicochemicalfactorsonadsorptionoftenshigellaflexneriphages
AT quiberoniandrea influenceofphysicochemicalfactorsonadsorptionoftenshigellaflexneriphages
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