Physical-mathematical model to predict the kinetic coagulation process by clotting activity of bacterial endopeptidases

A physical-mathematical model was used to evaluate the capability of an enzymatic pool of Bacillus sp. P7 (isolated from Piaractus mesopotamicus) to promote the bovine casein micelles coagulation. Experiments were designed to assess the effects of temperature, pH, and enzyme activity/mass of subs...

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Autores principales: Mancilla Canales, Manuel Arturo, Folmer Côrrea, Ana Paula, Riquelme, Bibiana Doris, Brandelli, Adriano, Risso, Patricia Hilda
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: MedCrave 2022
Materias:
Bacillus sp. P7
Bovine casein micelle
Enzymatic coagulation
Experimental design
Antioxidant activity
Acceso en línea:http://hdl.handle.net/2133/24936
http://hdl.handle.net/2133/24936
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-24936
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
topic Bacillus sp. P7
Bovine casein micelle
Enzymatic coagulation
Experimental design
Antioxidant activity
spellingShingle Bacillus sp. P7
Bovine casein micelle
Enzymatic coagulation
Experimental design
Antioxidant activity
Mancilla Canales, Manuel Arturo
Folmer Côrrea, Ana Paula
Riquelme, Bibiana Doris
Brandelli, Adriano
Risso, Patricia Hilda
Physical-mathematical model to predict the kinetic coagulation process by clotting activity of bacterial endopeptidases
topic_facet Bacillus sp. P7
Bovine casein micelle
Enzymatic coagulation
Experimental design
Antioxidant activity
description A physical-mathematical model was used to evaluate the capability of an enzymatic pool of Bacillus sp. P7 (isolated from Piaractus mesopotamicus) to promote the bovine casein micelles coagulation. Experiments were designed to assess the effects of temperature, pH, and enzyme activity/mass of substrate ratio on the kinetic parameters of the coagulation process and the microstructure of the obtained clots. Descriptive and predictive equations indicate that the temperature and the pH modified these parameters significantly. In optimal conditions, the clot’s mean pore size was 3.6 times smaller using chymosin. On the other hand, rheological measurements evidence a moderate elasticity of clot, which indicates the usefulness of P7 protease preparation as a clotting agent in spreadable or soft cheese manufacture. Also, the hydrolysis products, which are in the whey after casein micelles coagulation, demonstrated antioxidant activities. Equations to model and predict the process kinetics were combined with rheological and microstructure analyses of the obtained clots, and whey bioactivities were evaluated. Nevertheless, the use of P7PP requires further investigation concerning the stability of the enzyme preparation during storage, its performance, and how these variables could be related to the proposed models.
format article
artículo
publishedVersion
author Mancilla Canales, Manuel Arturo
Folmer Côrrea, Ana Paula
Riquelme, Bibiana Doris
Brandelli, Adriano
Risso, Patricia Hilda
author_facet Mancilla Canales, Manuel Arturo
Folmer Côrrea, Ana Paula
Riquelme, Bibiana Doris
Brandelli, Adriano
Risso, Patricia Hilda
author_sort Mancilla Canales, Manuel Arturo
title Physical-mathematical model to predict the kinetic coagulation process by clotting activity of bacterial endopeptidases
title_short Physical-mathematical model to predict the kinetic coagulation process by clotting activity of bacterial endopeptidases
title_full Physical-mathematical model to predict the kinetic coagulation process by clotting activity of bacterial endopeptidases
title_fullStr Physical-mathematical model to predict the kinetic coagulation process by clotting activity of bacterial endopeptidases
title_full_unstemmed Physical-mathematical model to predict the kinetic coagulation process by clotting activity of bacterial endopeptidases
title_sort physical-mathematical model to predict the kinetic coagulation process by clotting activity of bacterial endopeptidases
publisher MedCrave
publishDate 2022
url http://hdl.handle.net/2133/24936
http://hdl.handle.net/2133/24936
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bdutipo_str Repositorios
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