Identification of Novel Thermosensors in Gram-Positive Pathogens

Temperature is a crucial variable that every living organism, from bacteria to humans, need to sense and respond to in order to adapt and survive. In particular, pathogenic bacteria exploit host-temperature sensing as a cue for triggering virulence gene expression. Here, we have identified and charac...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Fernández, Pilar, Díaz, Alejandra Raquel, Ré, María Florencia, Porrini, Lucía, De Mendoza, Diego, Albanesi, Daniela, Mansilla, María Cecilia
Lenguaje:Inglés
Publicado: Frontiers Media 2022
Materias:
Thermosensor
Gram Positive Pathogen
ABC Transporter
Two Component System
Signalling
Acceso en línea:http://hdl.handle.net/2133/23310
http://hdl.handle.net/2133/23310
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-23310
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
topic Thermosensor
Gram Positive Pathogen
ABC Transporter
Two Component System
Signalling
spellingShingle Thermosensor
Gram Positive Pathogen
ABC Transporter
Two Component System
Signalling
Fernández, Pilar
Díaz, Alejandra Raquel
Ré, María Florencia
Porrini, Lucía
De Mendoza, Diego
Albanesi, Daniela
Mansilla, María Cecilia
Identification of Novel Thermosensors in Gram-Positive Pathogens
topic_facet Thermosensor
Gram Positive Pathogen
ABC Transporter
Two Component System
Signalling
description Temperature is a crucial variable that every living organism, from bacteria to humans, need to sense and respond to in order to adapt and survive. In particular, pathogenic bacteria exploit host-temperature sensing as a cue for triggering virulence gene expression. Here, we have identified and characterized two integral membrane thermosensor histidine kinases (HKs) from Gram-positive pathogens that exhibit high similarity to DesK, the extensively characterized cold sensor histidine kinase from Bacillus subtilis. Through in vivo experiments, we demonstrate that SA1313 from Staphylococcus aureus and BA5598 from Bacillus anthracis, which likely control the expression of putative ATP binding cassette (ABC) transporters, are regulated by environmental temperature. We show here that these HKs can phosphorylate the noncognate response regulator DesR, partner of DesK, both in vitro and in vivo, inducing in B. subtilis the expression of the des gene upon a cold shock. In addition, we report the characterization of another DesK homolog from B. subtilis, YvfT, also closely associated to an ABC transporter. Although YvfT phosphorylates DesR in vitro, this sensor kinase can only induce des expression in B. subtilis when overexpressed together with its cognate response regulator YvfU. This finding evidences a physiological mechanism to avoid cross talk with DesK after a temperature downshift. Finally, we present data suggesting that the HKs studied in this work appear to monitor different ranges of membrane lipid properties variations to mount adaptive responses upon cooling. Overall, our findings point out that bacteria have evolved sophisticated mechanisms to assure specificity in the response to environmental stimuli. These findings pave the way to understand thermosensing mediated by membrane proteins that could have important roles upon host invasion by bacterial pathogens.
author Fernández, Pilar
Díaz, Alejandra Raquel
Ré, María Florencia
Porrini, Lucía
De Mendoza, Diego
Albanesi, Daniela
Mansilla, María Cecilia
author_facet Fernández, Pilar
Díaz, Alejandra Raquel
Ré, María Florencia
Porrini, Lucía
De Mendoza, Diego
Albanesi, Daniela
Mansilla, María Cecilia
author_sort Fernández, Pilar
title Identification of Novel Thermosensors in Gram-Positive Pathogens
title_short Identification of Novel Thermosensors in Gram-Positive Pathogens
title_full Identification of Novel Thermosensors in Gram-Positive Pathogens
title_fullStr Identification of Novel Thermosensors in Gram-Positive Pathogens
title_full_unstemmed Identification of Novel Thermosensors in Gram-Positive Pathogens
title_sort identification of novel thermosensors in gram-positive pathogens
publisher Frontiers Media
publishDate 2022
url http://hdl.handle.net/2133/23310
http://hdl.handle.net/2133/23310
work_keys_str_mv AT fernandezpilar identificationofnovelthermosensorsingrampositivepathogens
AT diazalejandraraquel identificationofnovelthermosensorsingrampositivepathogens
AT remariaflorencia identificationofnovelthermosensorsingrampositivepathogens
AT porrinilucia identificationofnovelthermosensorsingrampositivepathogens
AT demendozadiego identificationofnovelthermosensorsingrampositivepathogens
AT albanesidaniela identificationofnovelthermosensorsingrampositivepathogens
AT mansillamariacecilia identificationofnovelthermosensorsingrampositivepathogens
bdutipo_str Repositorios
_version_ 1764820411951874049

Ejemplares similares