Aqueous Extract of Prosopis strombulifera (LAM) BENTH Induces Cytotoxic Effects against Tumor Cell Lines without Systemic Alterations in BALB/c Mice

Prosopis strombulifera (Lam.) Benth. is a rhizomatous shrub native to the northern and central zones of Argentina. The analgesic and antibiotic properties of this plant had been demonstrated but there are no previous reports of its cytotoxic activity. The goal of this work was to analyze the cytot...

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Autores principales: Hapon, María Belén, Hapon, María Vanda, Persia, Andres, Pochettino, Arístides A., Lucero, Gabriela Susana, Gamarra-Lúques, C.
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: OMICS Publishing Group 2021
Materias:
Prosopis strombulifera
Natural products
Cytotoxicity
Antiproliferative
Lethality
Mutagenicity
Acceso en línea:http://hdl.handle.net/2133/20911
http://hdl.handle.net/2133/20911
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-20911
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Prosopis strombulifera
Natural products
Cytotoxicity
Antiproliferative
Lethality
Mutagenicity
spellingShingle Prosopis strombulifera
Natural products
Cytotoxicity
Antiproliferative
Lethality
Mutagenicity
Hapon, María Belén
Hapon, María Vanda
Persia, Andres
Pochettino, Arístides A.
Lucero, Gabriela Susana
Gamarra-Lúques, C.
Aqueous Extract of Prosopis strombulifera (LAM) BENTH Induces Cytotoxic Effects against Tumor Cell Lines without Systemic Alterations in BALB/c Mice
topic_facet Prosopis strombulifera
Natural products
Cytotoxicity
Antiproliferative
Lethality
Mutagenicity
description Prosopis strombulifera (Lam.) Benth. is a rhizomatous shrub native to the northern and central zones of Argentina. The analgesic and antibiotic properties of this plant had been demonstrated but there are no previous reports of its cytotoxic activity. The goal of this work was to analyze the cytotoxic activity of P. strombulifera against HCT-116 and MCF-7 cell lines, and to evaluate toxic systemic effects in BALB/c mice. Changes induced by the aqueous extract from leaves on tumoral cell lines were studied by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide) tetrazolium reduction assay, Trypan blue dye exclusion assay, optical microscopy, Western Blot analysis of PCNA and cPARP, LDH activity, Ames´ test and clonogenic survival. Oral sub-chronic toxicity was assessed on BALB/c mice at concentrations up to 150 mg/animal/day. Analyses included animal/organs weight; erythrocytes, leukocytes and platelets number; and serological determinations of glucose, ASAT, ALAT, urea and creatinine. Extract has induced cytotoxicity, affecting proliferation and viability in both cell lines in a dose and time-response manner. IC50 was 2.25 μg/ml and LC50 5.05 μg/ml in HCT-116, while values were 3.01 μg/ml and 7.52 μg/ml, respectively, in MCF-7. In both cell lines, the antiproliferative effect was confirmed by reduction of PCNA protein expression. When LC50 concentrations were used, extract-induced necrosis (evidenced by the increase in extracellular LDH activity), apoptosis (increased cPARP protein expression) and clonogenic survival diminution. Mutagenic activity of extract was caused at concentrations of 500 μg/ml (99 and 64-fold higher than HCT-116 and MCF-7 LC50 concentrations). Animal studies demonstrated that no significant toxicity was induced. In conclusion, this is the first report of P. strombulifera cytotoxic activity against tumoral cell lines. Sub-chronic extract administration did not cause deleterious effects in vivo. Altogether, the presented results make P. strombulifera a promising natural product for cancer research and treatment.
format article
artículo
publishedVersion
author Hapon, María Belén
Hapon, María Vanda
Persia, Andres
Pochettino, Arístides A.
Lucero, Gabriela Susana
Gamarra-Lúques, C.
author_facet Hapon, María Belén
Hapon, María Vanda
Persia, Andres
Pochettino, Arístides A.
Lucero, Gabriela Susana
Gamarra-Lúques, C.
author_sort Hapon, María Belén
title Aqueous Extract of Prosopis strombulifera (LAM) BENTH Induces Cytotoxic Effects against Tumor Cell Lines without Systemic Alterations in BALB/c Mice
title_short Aqueous Extract of Prosopis strombulifera (LAM) BENTH Induces Cytotoxic Effects against Tumor Cell Lines without Systemic Alterations in BALB/c Mice
title_full Aqueous Extract of Prosopis strombulifera (LAM) BENTH Induces Cytotoxic Effects against Tumor Cell Lines without Systemic Alterations in BALB/c Mice
title_fullStr Aqueous Extract of Prosopis strombulifera (LAM) BENTH Induces Cytotoxic Effects against Tumor Cell Lines without Systemic Alterations in BALB/c Mice
title_full_unstemmed Aqueous Extract of Prosopis strombulifera (LAM) BENTH Induces Cytotoxic Effects against Tumor Cell Lines without Systemic Alterations in BALB/c Mice
title_sort aqueous extract of prosopis strombulifera (lam) benth induces cytotoxic effects against tumor cell lines without systemic alterations in balb/c mice
publisher OMICS Publishing Group
publishDate 2021
url http://hdl.handle.net/2133/20911
http://hdl.handle.net/2133/20911
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