Ribosomal protein RPL10A contributes to early plant development and abscisic acid-dependent responses in Arabidopsis

Plant ribosomal proteins play universal roles in translation, although they are also involved in developmental processes and hormone signaling pathways. Among Arabidopsis RPL10 family members, RPL10A exhibits the highest expression during germination and early development, suggesting that RPL1...

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Autores principales: Ramos, Rocío Soledad, Casati, Paula, Spampinato, Claudia P., Falcone Ferreyra, María Lorena
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: Frontiers Media 2021
Materias:
Seed Germination
Abscisic Acid (ABA)
Early Seedling Development
rpl10A Mutant
Acceso en línea:http://hdl.handle.net/2133/20766
http://hdl.handle.net/2133/20766
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-20766
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Seed Germination
Abscisic Acid (ABA)
Early Seedling Development
rpl10A Mutant
spellingShingle Seed Germination
Abscisic Acid (ABA)
Early Seedling Development
rpl10A Mutant
Ramos, Rocío Soledad
Casati, Paula
Spampinato, Claudia P.
Falcone Ferreyra, María Lorena
Ribosomal protein RPL10A contributes to early plant development and abscisic acid-dependent responses in Arabidopsis
topic_facet Seed Germination
Abscisic Acid (ABA)
Early Seedling Development
rpl10A Mutant
description Plant ribosomal proteins play universal roles in translation, although they are also involved in developmental processes and hormone signaling pathways. Among Arabidopsis RPL10 family members, RPL10A exhibits the highest expression during germination and early development, suggesting that RPL10A is the main contributor to these processes. In this work, we first analyzed RPL10A expression pattern in Arabidopsis thaliana using transgenic RPL10Apro:GUS plants. The gene exhibits a ubiquitous expression pattern throughout the plant, but it is most strongly expressed in undifferentiated tissues. Interestingly, gene expression was also detected in stomatal cells. We then examined protein function during seedling establishment and abscisic acid (ABA) response. Heterozygous rpl10A mutant plants show decreased ABA-sensitivity during seed germination, are impaired in early seedling and root development, and exhibit reduced ABA-inhibition of stomatal aperture under light conditions. Overexpression of RPL10A does not affect the germination and seedling growth, but RPL10A-overexpressing lines are more sensitive to ABA during early plant development and exhibit higher stomatal closure under light condition both with and without ABA treatment than wild type plants. Interestingly, RPL10A expression is induced by ABA. Together, we conclude that RPL10A could act as a positive regulator for ABA-dependent responses in Arabidopsis plants.
format article
artículo
publishedVersion
author Ramos, Rocío Soledad
Casati, Paula
Spampinato, Claudia P.
Falcone Ferreyra, María Lorena
author_facet Ramos, Rocío Soledad
Casati, Paula
Spampinato, Claudia P.
Falcone Ferreyra, María Lorena
author_sort Ramos, Rocío Soledad
title Ribosomal protein RPL10A contributes to early plant development and abscisic acid-dependent responses in Arabidopsis
title_short Ribosomal protein RPL10A contributes to early plant development and abscisic acid-dependent responses in Arabidopsis
title_full Ribosomal protein RPL10A contributes to early plant development and abscisic acid-dependent responses in Arabidopsis
title_fullStr Ribosomal protein RPL10A contributes to early plant development and abscisic acid-dependent responses in Arabidopsis
title_full_unstemmed Ribosomal protein RPL10A contributes to early plant development and abscisic acid-dependent responses in Arabidopsis
title_sort ribosomal protein rpl10a contributes to early plant development and abscisic acid-dependent responses in arabidopsis
publisher Frontiers Media
publishDate 2021
url http://hdl.handle.net/2133/20766
http://hdl.handle.net/2133/20766
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