Quorum and light signals modulate acetoin/butanediol catabolism in Acinetobacter spp

Acinetobacter spp. are found in all environments on Earth due to their extraordinary capacity to survive in the presence of physical and chemical stressors. In this study, we analyzed global gene expression in airborne Acinetobacter sp. strain 5-2Ac02 isolated from hospital environment in respons...

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Autores principales: Tuttobene, Marisel Romina, Fernández-García, Laura, Blasco, Lucía, Cribb, Pamela, Ambroa, Anton, Müller, Gabriela Leticia, Fernández-Cuenca, Felipe, Bleriot, Inés, Rodríguez, Ramiro Esteban, Barbosa, Beatriz G. V., Lopez-Rojas, Rafael, Trastoy, Rocío, López, María, Bou, Germán, Tomás, María, Mussi, María Alejandra
Formato: publishedVersion
Lenguaje:Inglés
Publicado: Frontiers Media 2021
Materias:
Acetoin
BlsA
AcoN
Light
Acinetobacter
Acceso en línea:http://hdl.handle.net/2133/20088
http://hdl.handle.net/2133/20088
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-20088
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Acetoin
BlsA
AcoN
Light
Acinetobacter
spellingShingle Acetoin
BlsA
AcoN
Light
Acinetobacter
Tuttobene, Marisel Romina
Fernández-García, Laura
Blasco, Lucía
Cribb, Pamela
Ambroa, Anton
Müller, Gabriela Leticia
Fernández-Cuenca, Felipe
Bleriot, Inés
Rodríguez, Ramiro Esteban
Barbosa, Beatriz G. V.
Lopez-Rojas, Rafael
Trastoy, Rocío
López, María
Bou, Germán
Tomás, María
Mussi, María Alejandra
Quorum and light signals modulate acetoin/butanediol catabolism in Acinetobacter spp
topic_facet Acetoin
BlsA
AcoN
Light
Acinetobacter
description Acinetobacter spp. are found in all environments on Earth due to their extraordinary capacity to survive in the presence of physical and chemical stressors. In this study, we analyzed global gene expression in airborne Acinetobacter sp. strain 5-2Ac02 isolated from hospital environment in response to quorum network modulators and found that they induced the expression of genes of the acetoin/butanediol catabolism, volatile compounds shown to mediate interkingdom interactions. Interestingly, the acoN gene, annotated as a putative transcriptional regulator, was truncated in the downstream regulatory region of the induced acetoin/butanediol cluster in Acinetobacter sp. strain 5-2Ac02, and its functioning as a negative regulator of this cluster integrating quorum signals was confirmed in Acinetobacter baumannii ATCC 17978. Moreover, we show that the acetoin catabolism is also induced by light and provide insights into the light transduction mechanism by showing that the photoreceptor BlsA interacts with and antagonizes the functioning of AcoN in A. baumannii, integrating also a temperature signal. The data support a model in which BlsA interacts with and likely sequesters AcoN at this condition, relieving acetoin catabolic genes from repression, and leading to better growth under blue light. This photoregulation depends on temperature, occurring at 23◦C but not at 30◦C. BlsA is thus a dual regulator, modulating different transcriptional regulators in the dark but also under blue light, representing thus a novel concept. The overall data show that quorum modulators as well as light regulate the acetoin catabolic cluster, providing a better understanding of environmental as well as clinical bacteria.
format publishedVersion
author Tuttobene, Marisel Romina
Fernández-García, Laura
Blasco, Lucía
Cribb, Pamela
Ambroa, Anton
Müller, Gabriela Leticia
Fernández-Cuenca, Felipe
Bleriot, Inés
Rodríguez, Ramiro Esteban
Barbosa, Beatriz G. V.
Lopez-Rojas, Rafael
Trastoy, Rocío
López, María
Bou, Germán
Tomás, María
Mussi, María Alejandra
author_facet Tuttobene, Marisel Romina
Fernández-García, Laura
Blasco, Lucía
Cribb, Pamela
Ambroa, Anton
Müller, Gabriela Leticia
Fernández-Cuenca, Felipe
Bleriot, Inés
Rodríguez, Ramiro Esteban
Barbosa, Beatriz G. V.
Lopez-Rojas, Rafael
Trastoy, Rocío
López, María
Bou, Germán
Tomás, María
Mussi, María Alejandra
author_sort Tuttobene, Marisel Romina
title Quorum and light signals modulate acetoin/butanediol catabolism in Acinetobacter spp
title_short Quorum and light signals modulate acetoin/butanediol catabolism in Acinetobacter spp
title_full Quorum and light signals modulate acetoin/butanediol catabolism in Acinetobacter spp
title_fullStr Quorum and light signals modulate acetoin/butanediol catabolism in Acinetobacter spp
title_full_unstemmed Quorum and light signals modulate acetoin/butanediol catabolism in Acinetobacter spp
title_sort quorum and light signals modulate acetoin/butanediol catabolism in acinetobacter spp
publisher Frontiers Media
publishDate 2021
url http://hdl.handle.net/2133/20088
http://hdl.handle.net/2133/20088
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