Induced folding in RNA recognition by Arabidopsis thaliana DCL1

DCL1 is the ribonuclease that carries out miRNA biogenesis in plants. The enzyme has two tandem double stranded RNA binding domains (dsRBDs) in its C-terminus. Here we show that the first of these domains binds precursor RNA fragments when isolated and cooperates with the second domain in the reco...

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Detalles Bibliográficos
Autores principales:	Suárez, Irina Paula, Burdisso, Paula, Benoit, Matthieu P. M. H., Boisbouvier, Jérôme, Rasia, Rodolfo M.
Formato:	article artículo publishedVersion
Lenguaje:	Inglés
Publicado:	Oxford University Press 2021
Materias:	Dcl1 MicroRNAs Double-stranded RNA-Binding Domain (dsRBD) Intrinsically Disordered Proteins
Acceso en línea:	http://hdl.handle.net/2133/20017 http://hdl.handle.net/2133/20017
Aporte de:	Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario

id	I15-R121-2133-20017
record_format	dspace
institution	Universidad Nacional de Rosario
institution_str	I-15
repository_str	R-121
collection	Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language	Inglés
topic	Dcl1 MicroRNAs Double-stranded RNA-Binding Domain (dsRBD) Intrinsically Disordered Proteins
spellingShingle	Dcl1 MicroRNAs Double-stranded RNA-Binding Domain (dsRBD) Intrinsically Disordered Proteins Suárez, Irina Paula Burdisso, Paula Benoit, Matthieu P. M. H. Boisbouvier, Jérôme Rasia, Rodolfo M. Induced folding in RNA recognition by Arabidopsis thaliana DCL1
topic_facet	Dcl1 MicroRNAs Double-stranded RNA-Binding Domain (dsRBD) Intrinsically Disordered Proteins
description	DCL1 is the ribonuclease that carries out miRNA biogenesis in plants. The enzyme has two tandem double stranded RNA binding domains (dsRBDs) in its C-terminus. Here we show that the first of these domains binds precursor RNA fragments when isolated and cooperates with the second domain in the recognition of substrate RNA. Remarkably, despite showing RNA binding activity, this domain is intrinsically disordered. We found that it acquires a folded conformation when bound to its substrate, being the first report of a complete dsRBD folding upon binding. The free unfolded form shows tendency to adopt folded conformations, and goes through an unfolded bound state prior to the folding event. The significance of these results is discussed by comparison with the behavior of other dsRBDs.
format	article artículo publishedVersion
author	Suárez, Irina Paula Burdisso, Paula Benoit, Matthieu P. M. H. Boisbouvier, Jérôme Rasia, Rodolfo M.
author_facet	Suárez, Irina Paula Burdisso, Paula Benoit, Matthieu P. M. H. Boisbouvier, Jérôme Rasia, Rodolfo M.
author_sort	Suárez, Irina Paula
title	Induced folding in RNA recognition by Arabidopsis thaliana DCL1
title_short	Induced folding in RNA recognition by Arabidopsis thaliana DCL1
title_full	Induced folding in RNA recognition by Arabidopsis thaliana DCL1
title_fullStr	Induced folding in RNA recognition by Arabidopsis thaliana DCL1
title_full_unstemmed	Induced folding in RNA recognition by Arabidopsis thaliana DCL1
title_sort	induced folding in rna recognition by arabidopsis thaliana dcl1
publisher	Oxford University Press
publishDate	2021
url	http://hdl.handle.net/2133/20017 http://hdl.handle.net/2133/20017
work_keys_str_mv	AT suarezirinapaula inducedfoldinginrnarecognitionbyarabidopsisthalianadcl1 AT burdissopaula inducedfoldinginrnarecognitionbyarabidopsisthalianadcl1 AT benoitmatthieupmh inducedfoldinginrnarecognitionbyarabidopsisthalianadcl1 AT boisbouvierjerome inducedfoldinginrnarecognitionbyarabidopsisthalianadcl1 AT rasiarodolfom inducedfoldinginrnarecognitionbyarabidopsisthalianadcl1
bdutipo_str	Repositorios
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Induced folding in RNA recognition by Arabidopsis thaliana DCL1

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