Efficiency and precision of microRNA biogenesis modes in plants

Many evolutionarily conserved microRNAs (miRNAs) in plants regulate transcription factors with key functions in development. Hence, mutations in the core components of the miRNA biogenesis machinery cause strong growth defects. An essential aspect of miRNA biogenesis is the precise excision of t...

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Autores principales: Moro, Belén, Chorostecki, Uciel Pablo, Arikit, Siwaret, Suárez, Irina Paula, Höbartner, Claudia, Rasia, Rodolfo M., Meyers, Blake C., Palatnik, Javier F.
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: Oxford University Press 2021
Materias:
Gene Expression Regulation, Plant
Transcription, Genetic
Binding Sites
Transgenes
RNA-Binding Proteins
RNA, Double-Stranded
Acceso en línea:http://hdl.handle.net/2133/20015
http://hdl.handle.net/2133/20015
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-20015
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
topic Gene Expression Regulation, Plant
Transcription, Genetic
Binding Sites
Transgenes
RNA-Binding Proteins
RNA, Double-Stranded
spellingShingle Gene Expression Regulation, Plant
Transcription, Genetic
Binding Sites
Transgenes
RNA-Binding Proteins
RNA, Double-Stranded
Moro, Belén
Chorostecki, Uciel Pablo
Arikit, Siwaret
Suárez, Irina Paula
Höbartner, Claudia
Rasia, Rodolfo M.
Meyers, Blake C.
Palatnik, Javier F.
Efficiency and precision of microRNA biogenesis modes in plants
topic_facet Gene Expression Regulation, Plant
Transcription, Genetic
Binding Sites
Transgenes
RNA-Binding Proteins
RNA, Double-Stranded
description Many evolutionarily conserved microRNAs (miRNAs) in plants regulate transcription factors with key functions in development. Hence, mutations in the core components of the miRNA biogenesis machinery cause strong growth defects. An essential aspect of miRNA biogenesis is the precise excision of the small RNA from its precursor. In plants, miRNA precursors are largely variable in size and shape and can be processed by different modes. Here, we optimized an approach to detect processing intermediates during miRNA biogenesis. We characterized a miRNA whose processing is triggered by a terminal branched loop. Plant miRNA processing can be initiated by internal bubbles, small terminal loops or branched loops followed by dsRNA segments of 15–17 bp. Interestingly, precision and efficiency vary with the processing modes. Despite the various potential structural determinants present in a single a miRNA precursor, DCL1 is mostly guided by a predominant structural region in each precursor in wildtype plants. However, our studies in fiery1, hyl1 and se mutants revealed the existence of cleavage signatures consistent with the recognition of alternative processing determinants. The results provide a general view of the mechanisms underlying the specificity of miRNA biogenesis in plants.
format article
artículo
publishedVersion
author Moro, Belén
Chorostecki, Uciel Pablo
Arikit, Siwaret
Suárez, Irina Paula
Höbartner, Claudia
Rasia, Rodolfo M.
Meyers, Blake C.
Palatnik, Javier F.
author_facet Moro, Belén
Chorostecki, Uciel Pablo
Arikit, Siwaret
Suárez, Irina Paula
Höbartner, Claudia
Rasia, Rodolfo M.
Meyers, Blake C.
Palatnik, Javier F.
author_sort Moro, Belén
title Efficiency and precision of microRNA biogenesis modes in plants
title_short Efficiency and precision of microRNA biogenesis modes in plants
title_full Efficiency and precision of microRNA biogenesis modes in plants
title_fullStr Efficiency and precision of microRNA biogenesis modes in plants
title_full_unstemmed Efficiency and precision of microRNA biogenesis modes in plants
title_sort efficiency and precision of microrna biogenesis modes in plants
publisher Oxford University Press
publishDate 2021
url http://hdl.handle.net/2133/20015
http://hdl.handle.net/2133/20015
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