KatG, the bifunctional catalase of xanthomonas citri subsp. citri, responds to hydrogen peroxide and contributes to epiphytic survival on citrus leaves

Xanthomonas citri subsp. citri (Xcc) is the bacterium responsible for citrus canker. This bacterium is exposed to reactive oxygen species (ROS) at different points during its life cycle, including those normally produced by aerobic respiration or upon exposition to ultraviolet (UV) radiation. More...

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Autores principales: Tondo, María Laura, Delprato, María Laura, Kraiselburd, Ivana, Fernández Zenoff, María Verónica, Farías, María Eugenia, Orellano, Elena G.
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: Public Library of Science (PLOS) 2021
Materias:
Xanthomonas citri subsp. citri
Hydrogen Peroxide
KatG
Catalase
Plant Leaves
Epiphytic Survival
Citrus Canker
Acceso en línea:http://hdl.handle.net/2133/19678
http://hdl.handle.net/2133/19678
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-19678
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Xanthomonas citri subsp. citri
Hydrogen Peroxide
KatG
Catalase
Plant Leaves
Epiphytic Survival
Citrus Canker
spellingShingle Xanthomonas citri subsp. citri
Hydrogen Peroxide
KatG
Catalase
Plant Leaves
Epiphytic Survival
Citrus Canker
Tondo, María Laura
Delprato, María Laura
Kraiselburd, Ivana
Fernández Zenoff, María Verónica
Farías, María Eugenia
Orellano, Elena G.
KatG, the bifunctional catalase of xanthomonas citri subsp. citri, responds to hydrogen peroxide and contributes to epiphytic survival on citrus leaves
topic_facet Xanthomonas citri subsp. citri
Hydrogen Peroxide
KatG
Catalase
Plant Leaves
Epiphytic Survival
Citrus Canker
description Xanthomonas citri subsp. citri (Xcc) is the bacterium responsible for citrus canker. This bacterium is exposed to reactive oxygen species (ROS) at different points during its life cycle, including those normally produced by aerobic respiration or upon exposition to ultraviolet (UV) radiation. Moreover, ROS are key components of the host immune response. Among enzymatic ROS-detoxifying mechanisms, catalases eliminate H2O2, avoiding the potential damage caused by this specie. Xcc genome includes four catalase genes. In this work, we studied the physiological role of KatG, the only bifunctional catalase of Xcc, through the construction and characterization of a modified strain (XcckatG), carrying an insertional mutation in the katG gene. First, we evaluated the involvement of KatG in the bacterial adaptive response to H2O2. XcckatG cultures exhibited lower catalase activity than those of the wild-type strain, and this activity was not induced upon treatment with sub-lethal doses of H2O2. Moreover, the KatG-deficient mutant exhibited decreased tolerance to H2O2 toxicity compared to wild-type cells and accumulated high intracellular levels of peroxides upon exposure to sub-lethal concentrations of H2O2. To further study the role of KatG in Xcc physiology, we evaluated bacterial survival upon exposure to UV-A or UV-B radiation. In both conditions, XcckatG showed a high mortality in comparison to Xcc wild-type. Finally, we studied the development of bacterial biofilms. While structured biofilms were observed for the Xcc wild-type, the development of these structures was impaired for XcckatG. Based on these results, we demonstrated that KatG is responsible for Xcc adaptive response to H2O2 and a key component of the bacterial response to oxidative stress. Moreover, this enzyme plays an important role during Xcc epiphytic survival, being essential for biofilm formation and UV resistance.
format article
artículo
publishedVersion
author Tondo, María Laura
Delprato, María Laura
Kraiselburd, Ivana
Fernández Zenoff, María Verónica
Farías, María Eugenia
Orellano, Elena G.
author_facet Tondo, María Laura
Delprato, María Laura
Kraiselburd, Ivana
Fernández Zenoff, María Verónica
Farías, María Eugenia
Orellano, Elena G.
author_sort Tondo, María Laura
title KatG, the bifunctional catalase of xanthomonas citri subsp. citri, responds to hydrogen peroxide and contributes to epiphytic survival on citrus leaves
title_short KatG, the bifunctional catalase of xanthomonas citri subsp. citri, responds to hydrogen peroxide and contributes to epiphytic survival on citrus leaves
title_full KatG, the bifunctional catalase of xanthomonas citri subsp. citri, responds to hydrogen peroxide and contributes to epiphytic survival on citrus leaves
title_fullStr KatG, the bifunctional catalase of xanthomonas citri subsp. citri, responds to hydrogen peroxide and contributes to epiphytic survival on citrus leaves
title_full_unstemmed KatG, the bifunctional catalase of xanthomonas citri subsp. citri, responds to hydrogen peroxide and contributes to epiphytic survival on citrus leaves
title_sort katg, the bifunctional catalase of xanthomonas citri subsp. citri, responds to hydrogen peroxide and contributes to epiphytic survival on citrus leaves
publisher Public Library of Science (PLOS)
publishDate 2021
url http://hdl.handle.net/2133/19678
http://hdl.handle.net/2133/19678
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