TAT-mediated transduction of bacterial redox proteins generates a cytoprotective effect on neuronal cells

Cell penetrating peptides, also known as protein transduction domains, have the capacity to ubiquitously cross cellular membranes carrying many different cargos with negligible cytotoxicity. As a result, they have emerged as a powerful tool for macromolecular deliverybased therapies. In this study,...

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Detalles Bibliográficos
Autores principales: Balaban, Cecilia Lucía, Banchio, Claudia, Ceccarelli, Eduardo Augusto
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: Public Library of Science (PLOS) 2021
Materias:
TAT
Regulatory Protein
Bacteria
Redox Proteins
Cytoprotection
Neurons
Oxidative Stress
Acceso en línea:http://hdl.handle.net/2133/19675
http://hdl.handle.net/2133/19675
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-19675
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
topic TAT
Regulatory Protein
Bacteria
Redox Proteins
Cytoprotection
Neurons
Oxidative Stress
spellingShingle TAT
Regulatory Protein
Bacteria
Redox Proteins
Cytoprotection
Neurons
Oxidative Stress
Balaban, Cecilia Lucía
Banchio, Claudia
Ceccarelli, Eduardo Augusto
TAT-mediated transduction of bacterial redox proteins generates a cytoprotective effect on neuronal cells
topic_facet TAT
Regulatory Protein
Bacteria
Redox Proteins
Cytoprotection
Neurons
Oxidative Stress
description Cell penetrating peptides, also known as protein transduction domains, have the capacity to ubiquitously cross cellular membranes carrying many different cargos with negligible cytotoxicity. As a result, they have emerged as a powerful tool for macromolecular deliverybased therapies. In this study, catalytically active bacterial Ferredoxin-NADP+ reductase (LepFNR) and Heme oxygenase (LepHO) fused to the HIV TAT-derived protein transduction peptide (TAT) were efficiently transduced to neuroblastoma SHSY-5Y cells. Proteins entered the cells through an endocytic pathway showing a time/concentration dependent mechanism that was clearly modulated by the nature of the cargo protein. Since ferredoxinNADP+ reductases and heme oxygenases have been implicated in mechanisms of oxidative stress defense, neuroblastoma cells simultaneously transduced with TAT-LepFNR and TAT-LepHO were challenged by H2O2 incubations to judge the cytoprotective power of these bacterial enzymes. Accumulation of reactive oxygen species was significantly reduced in these transduced neuronal cells. Moreover, measurements of metabolic viability, membrane integrity, and cell survival indicated that these cells showed a better tolerance to oxidative stress. Our results open the possibility for the application of transducible active redox proteins to overcome the damage elicited by oxidative stress in cells and tissues.
format article
artículo
publishedVersion
author Balaban, Cecilia Lucía
Banchio, Claudia
Ceccarelli, Eduardo Augusto
author_facet Balaban, Cecilia Lucía
Banchio, Claudia
Ceccarelli, Eduardo Augusto
author_sort Balaban, Cecilia Lucía
title TAT-mediated transduction of bacterial redox proteins generates a cytoprotective effect on neuronal cells
title_short TAT-mediated transduction of bacterial redox proteins generates a cytoprotective effect on neuronal cells
title_full TAT-mediated transduction of bacterial redox proteins generates a cytoprotective effect on neuronal cells
title_fullStr TAT-mediated transduction of bacterial redox proteins generates a cytoprotective effect on neuronal cells
title_full_unstemmed TAT-mediated transduction of bacterial redox proteins generates a cytoprotective effect on neuronal cells
title_sort tat-mediated transduction of bacterial redox proteins generates a cytoprotective effect on neuronal cells
publisher Public Library of Science (PLOS)
publishDate 2021
url http://hdl.handle.net/2133/19675
http://hdl.handle.net/2133/19675
work_keys_str_mv AT balabancecilialucia tatmediatedtransductionofbacterialredoxproteinsgeneratesacytoprotectiveeffectonneuronalcells
AT banchioclaudia tatmediatedtransductionofbacterialredoxproteinsgeneratesacytoprotectiveeffectonneuronalcells
AT ceccarellieduardoaugusto tatmediatedtransductionofbacterialredoxproteinsgeneratesacytoprotectiveeffectonneuronalcells
bdutipo_str Repositorios
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