KDM2B regulates choline kinase expression and neuronal differentiation of neuroblastoma cells

The process of neuronal differentiation is associated with neurite elongation and membrane biogenesis, and phosphatidylcholine (PtdCho) is the major membrane phospholipid in mammalian cells. During neuroblast differentiation, the transcription of two genes involved in PtdCho biosynthesis are stimu...

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Autores principales: Domizi, Pablo, Malizia, Florencia, Chazarreta-Cifre, Lorena, Diacovich, Lautaro, Banchio, Claudia
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: Public Library of Science (PLOS) 2021
Materias:
KDM2B Gene
Choline Kinase Expression
Neuronal Differentiation
Neuroblastoma Cells
Acceso en línea:http://hdl.handle.net/2133/19669
http://hdl.handle.net/2133/19669
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-19669
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic KDM2B Gene
Choline Kinase Expression
Neuronal Differentiation
Neuroblastoma Cells
spellingShingle KDM2B Gene
Choline Kinase Expression
Neuronal Differentiation
Neuroblastoma Cells
Domizi, Pablo
Malizia, Florencia
Chazarreta-Cifre, Lorena
Diacovich, Lautaro
Banchio, Claudia
KDM2B regulates choline kinase expression and neuronal differentiation of neuroblastoma cells
topic_facet KDM2B Gene
Choline Kinase Expression
Neuronal Differentiation
Neuroblastoma Cells
description The process of neuronal differentiation is associated with neurite elongation and membrane biogenesis, and phosphatidylcholine (PtdCho) is the major membrane phospholipid in mammalian cells. During neuroblast differentiation, the transcription of two genes involved in PtdCho biosynthesis are stimulated: Chka gene for choline kinase (CK) alpha isoform and Pcyt1a gene for CTP:phosphocholine cytidylyltransferase (CCT) alpha isoform. Here we show that CKα is essential for neuronal differentiation. In addition, we demonstrated that KDM2B regulates CKα expression and, as a consequence, neuronal differentiation. This factor is up-regulated in the course of the neuroblasts proliferative and undifferentiated state and down-regulated during differentiation induced by retinoic acid (RA). During proliferation, KDM2B binds to the Box2 located in the Chka promoter repressing its transcription. Interestingly, KDM2B knockdown enhances the levels of CKα expression in neuroblast cells and induces neuronal differentiation even in the absence of RA. These results suggest that KDM2B is required for the appropriate regulation of CKα during neuronal differentiation and to the maintaining of the undifferentiated stage of neuroblast cells.
format article
artículo
publishedVersion
author Domizi, Pablo
Malizia, Florencia
Chazarreta-Cifre, Lorena
Diacovich, Lautaro
Banchio, Claudia
author_facet Domizi, Pablo
Malizia, Florencia
Chazarreta-Cifre, Lorena
Diacovich, Lautaro
Banchio, Claudia
author_sort Domizi, Pablo
title KDM2B regulates choline kinase expression and neuronal differentiation of neuroblastoma cells
title_short KDM2B regulates choline kinase expression and neuronal differentiation of neuroblastoma cells
title_full KDM2B regulates choline kinase expression and neuronal differentiation of neuroblastoma cells
title_fullStr KDM2B regulates choline kinase expression and neuronal differentiation of neuroblastoma cells
title_full_unstemmed KDM2B regulates choline kinase expression and neuronal differentiation of neuroblastoma cells
title_sort kdm2b regulates choline kinase expression and neuronal differentiation of neuroblastoma cells
publisher Public Library of Science (PLOS)
publishDate 2021
url http://hdl.handle.net/2133/19669
http://hdl.handle.net/2133/19669
work_keys_str_mv AT domizipablo kdm2bregulatescholinekinaseexpressionandneuronaldifferentiationofneuroblastomacells
AT maliziaflorencia kdm2bregulatescholinekinaseexpressionandneuronaldifferentiationofneuroblastomacells
AT chazarretacifrelorena kdm2bregulatescholinekinaseexpressionandneuronaldifferentiationofneuroblastomacells
AT diacovichlautaro kdm2bregulatescholinekinaseexpressionandneuronaldifferentiationofneuroblastomacells
AT banchioclaudia kdm2bregulatescholinekinaseexpressionandneuronaldifferentiationofneuroblastomacells
bdutipo_str Repositorios
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