Prospecting biotechnologically-relevant monooxygenases from cold sediment metagenomes: an In silico approach

The goal of this work was to identify sequences encoding monooxygenase biocatalysts with novel features by in silico mining an assembled metagenomic dataset of polar and subpolar marine sediments. The targeted enzyme sequences were Baeyer–Villiger and bacterial cytochrome P450 monooxygenases (CYP...

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Detalles Bibliográficos
Autores principales:	Musumeci, Matías A., Lozada, Mariana, Rial, Daniela V., Mac Cormack, Walter P., Jansson, Janet K., Sjöling, Sara, Carroll, JoLynn, Dionisi, Hebe
Formato:	article artículo publishedVersion
Lenguaje:	Inglés
Publicado:	MDPI 2021
Materias:	Bacterial Cytochrome P450 Baeyer–Villiger Monooxygenases Bioprospecting Enzymes Phylogeny Molecular Modeling
Acceso en línea:	http://hdl.handle.net/2133/19665 http://hdl.handle.net/2133/19665
Aporte de:	Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario

id	I15-R121-2133-19665
record_format	dspace
institution	Universidad Nacional de Rosario
institution_str	I-15
repository_str	R-121
collection	Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language	Inglés
orig_language_str_mv	eng
topic	Bacterial Cytochrome P450 Baeyer–Villiger Monooxygenases Bioprospecting Enzymes Phylogeny Molecular Modeling
spellingShingle	Bacterial Cytochrome P450 Baeyer–Villiger Monooxygenases Bioprospecting Enzymes Phylogeny Molecular Modeling Musumeci, Matías A. Lozada, Mariana Rial, Daniela V. Mac Cormack, Walter P. Jansson, Janet K. Sjöling, Sara Carroll, JoLynn Dionisi, Hebe Prospecting biotechnologically-relevant monooxygenases from cold sediment metagenomes: an In silico approach
topic_facet	Bacterial Cytochrome P450 Baeyer–Villiger Monooxygenases Bioprospecting Enzymes Phylogeny Molecular Modeling
description	The goal of this work was to identify sequences encoding monooxygenase biocatalysts with novel features by in silico mining an assembled metagenomic dataset of polar and subpolar marine sediments. The targeted enzyme sequences were Baeyer–Villiger and bacterial cytochrome P450 monooxygenases (CYP153). These enzymes have wide-ranging applications, from the synthesis of steroids, antibiotics, mycotoxins and pheromones to the synthesis of monomers for polymerization and anticancer precursors, due to their extraordinary enantio-, regio-, and chemo- selectivity that are valuable features for organic synthesis. Phylogenetic analyses were used to select the most divergent sequences affiliated to these enzyme families among the 264 putative monooxygenases recovered from the ~14 million protein-coding sequences in the assembled metagenome dataset. Three-dimensional structure modeling and docking analysis suggested features useful in biotechnological applications in five metagenomic sequences, such as wide substrate range, novel substrate specificity or regioselectivity. Further analysis revealed structural features associated with psychrophilic enzymes, such as broader substrate accessibility, larger catalytic pockets or low domain interactions, suggesting that they could be applied in biooxidations at room or low temperatures, saving costs inherent to energy consumption. This work allowed the identification of putative enzyme candidates with promising features from metagenomes, providing a suitable starting point for further developments.
format	article artículo publishedVersion
author	Musumeci, Matías A. Lozada, Mariana Rial, Daniela V. Mac Cormack, Walter P. Jansson, Janet K. Sjöling, Sara Carroll, JoLynn Dionisi, Hebe
author_facet	Musumeci, Matías A. Lozada, Mariana Rial, Daniela V. Mac Cormack, Walter P. Jansson, Janet K. Sjöling, Sara Carroll, JoLynn Dionisi, Hebe
author_sort	Musumeci, Matías A.
title	Prospecting biotechnologically-relevant monooxygenases from cold sediment metagenomes: an In silico approach
title_short	Prospecting biotechnologically-relevant monooxygenases from cold sediment metagenomes: an In silico approach
title_full	Prospecting biotechnologically-relevant monooxygenases from cold sediment metagenomes: an In silico approach
title_fullStr	Prospecting biotechnologically-relevant monooxygenases from cold sediment metagenomes: an In silico approach
title_full_unstemmed	Prospecting biotechnologically-relevant monooxygenases from cold sediment metagenomes: an In silico approach
title_sort	prospecting biotechnologically-relevant monooxygenases from cold sediment metagenomes: an in silico approach
publisher	MDPI
publishDate	2021
url	http://hdl.handle.net/2133/19665 http://hdl.handle.net/2133/19665
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bdutipo_str	Repositorios
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Prospecting biotechnologically-relevant monooxygenases from cold sediment metagenomes: an In silico approach

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